Climatic adaptation of bovine subspecies monitored by seminal characteristics during winter months in tropical region / Adaptação climática das subespécies bovinas monitoradas pelas características seminais durante meses de inverno em região tropical

Ana Beatriz Marques de Almeida, Myrian Megumy Tsunokawa Hidalgo, Fábio Morotti, Maria Isabel Mello Martins


The objective of this study was to evaluate parameters indicative of sperm quality (motility, vigor, and morphology) during the winter months in South-Mato Grosso, from Bos taurus and Bos indicus bulls, to analyze the climate influences in seminal changes. The ejaculate collects were performed on alternate days, using an artificial vagina, at a central for semen collection and bovine reproduction in the Midwest region from Brazil. In total, 122 ejaculates were evaluated, from 10 bulls, six Bos indicus and four Bos taurus. The ejaculates were submitted to evaluation of motility and vigor in phase contrast microscopy, and the sperm morphology was analyzed in a humid chamber. Samples of semen that presented 60% of motility and score 3 in vigor analysis were selected for freezing. After thawing, the motility and vigor were again evaluated. The statistical analysis was performed using a generalized linear mixed model (GLIMMIX) (P ? 0.05). Fresh (P = 0.016) and post-thawing vigor (P = 0.014), as well as the proportion of minor defects were influenced by the climate (P = 0.001) over the months. Among the subspecies (indicus x taurus), differences were observed only for minor morphological defects (3.27% indicus x 2.6% taurus; P = 0.009). It was concluded that, independent of the subspecies, freezing decreases the seminal parameters evaluated. In addition, indicus animals presented a lower proportion of minor defects, and taurine bulls seemed to be better adapted to the winter, although the climate did not generate significant alterations in ejaculate quality.


bulls, cryopreservation, semen, sperm motility.

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Abavisani, A., Arshami, J., Naserian, A. A., Sheikholeslami Kandelousi, M. A., & Azizzadeh, M. (2013). Quality of bovine chilled or frozen-thawed semen after addition of omega-3 fatty acids supplementation to extender. International Journal of Fertility and Sterility, 7(3), 161–168.

Barth, A. D., & Bowman, P. A. (1994). The sequential appearance of sperm abnormalities after scrotal insulation or dexamethasone treatment in bulls. Canadian Veterinary Journal, 35(2), 93–102.

Bergstein, T. G., Weiss, R. R., & Bicudo, S. D. (2014). Técnicas de análise de sêmen. 189–194.

Brito, L. F. C., Silva, A. E. D. F., Barbosa, R. T., Unanian, M. M., & Kastelic, J. P. (2003). Effects of scrotal insulation on sperm production, semen quality, and testicular echotexture in Bos indicus and Bos indicus x Bos taurus bulls. Animal Reproduction Science, 79(1–2), 1–15.

CBRA. (2013). Manual para exame andrológico e avaliação de sêmen animal. (3rd ed.; Colégio Brasileiro de Reprodução Animal, ed.). Belo Horizonte.

Celeghini, E. C. C., Arruda, R. P. De, Rodriguez, S. A. F., Santos, F. B. Dos, Alves, M. B. R., & Oliveira, B. M. M. De. (2017). Impacto da qualidade do sêmen sobre a fertilidade a campo em bovinos. Revista Brasileira de Reprodução Animal, 41(1), 40–45.

CEMTEC, C. de M. do T. e do C. de M. (2014). Banco de Dados - Boletim mensal. Retrieved from

Chacón, J., Pérez, E., & Rodríguez-Martínez, H. (2002). Seasonal variations in testicular consistency, scrotal circumference and spermiogramme parameters of extensively reared Brahman (Bos indicus) bulls in the tropics. Theriogenology, 58(1), 41–50.

Chacur, M. G. M., Mizusaki, K. T., Santos, F. H., Cesare, A. G., Oba, E., & Ramos, A. A. (2012). Influência da estação do ano nas características do sêmen e na concentração de hormônios em touros Nelore e Simental. Arq. Bras. Med. Vet. Zootec, 64(3), 540–546.

D’Andre, H. C., Kugonza, D. R., Amahoro, E., Ingabire, C., Niyiragira, V., Myambi, C., … Gahakwa, D. (2017). Influence of breed, season and age on quality bovine semen used for artificial insemination. International Journal of Livestock Production, 8(6), 72–78.

Fraser, L., Strzezek, J., & Kordan, W. (2014). Post-thaw sperm characteristics following long-term storage of boar semen in liquid nitrogen. Animal Reproduction Science, 147(3–4), 119–127.

Garcia, A. R. (2017). Degeneração testicular: um problema superado ou ainda um dilema? Revista Brasileira de Reprodução Animal, 41(1), 33–39.

Gopinathan, A., Sivaselvam, S. N., Kanniappan Karthickeyan, S., & Venkataramanan, R. (2018). Influence of Non-Genetic Factors on Semen Quality Parameters in Crossbred Jersey (Bos taurus x Bos indicus) Bulls. International Journal of Current Microbiology and Applied Sciences, 7(04), 2994–3004.

Hansen, P. J. (2004). Physiological and cellular adaptations of zebu cattle to thermal stress. Animal Reproduction Science, 82–83, 349–360.

Igna, V., Moje, A., Mircu, C., Roman, M., & Ghiurca, C. (2010). the Influence of Some Environmental Factors and Age on Semen Production of Fleckvieh Bulls. XLIII(2), 56–63.

Kaka, A., Haron, W., Yusoff, R., Yimer, N., Khumran, A. M., Memon, A. A., … Ebrahimi, M. (2017). Frozen-thawed quality of bull semen after combined supplementation of docosahexaenoic acid and alpha linolenic acid into tris based semen extender. Pakistan Journal of Zoology, 49(6), 2051–2055.

Koivisto, M. B., Costa, M. T. A., Perri, S. H. V., & Vicente, W. R. R. (2009). The effect of season on semen characteristics and freezability in bos indicus and bos taurus bulls in the Southeastern Region of Brazil. Reproduction in Domestic Animals, 44(4), 587–592.

Köppen, W. (1918). Classification of climates according to temperature, precipitation and seasonal cycle. Petermanns Geogr. Mitt., 64, 193–203.

Krishnan, G., Bagath, M., Pragna, P., Vidya, M. K., Aleena, J., Archana, P. R., … Bhatta, R. (2017). Mitigation of the Heat Stress Impact in Livestock Reproduction. Theriogenology.

Queiroz, V. L. D., Filho, L. C. C. da C., Rosa, L. D. S., Zúccari, C. E. S. N., & Costa e Silva, E. V. da. (2015). Sazonalidade Na Congelabilidade De Sêmen Bovino. Arquivos de Ciências Veterinárias e Zoologia Da UNIPAR, 18(1), 39–47.

Ram, K. L., Tiwari, R. P., Mishra, G. K., Sahasrabudhe, S. A., & Nair, A. K. (2017). Effect of heat stress on seminal characteristics of murrah buffalo bull semen. Buffalo Bulletin, 36(2), 369–378.

Ringwelski, J. M., Beever, J. E., & Knox, R. V. (2013). Effect of interval between inseminations when using frozen-thawed boar sperm on fertility and fetal paternity in mature gilts. Animal Reproduction Science, 137(3–4), 197–204.

Rodrigues, L. H., Deragon, L. A. G., Silva, A. E. D. F., Vieira, F. V., Kastelic, J. P., & Brito, L. F. C. (2002). Effects of environmental factors, age and genotype on sperm production and semen quality in Bos indicus and Bos taurus AI bulls in Brazil. Animal Reproduction Science, 70(3–4), 181–190.

Romanello, N., de Brito Lourenço Junior, J., Barioni Junior, W., Brandão, F. Z., Marcondes, C. R., Pezzopane, J. R. M., … Garcia, A. R. (2018). Thermoregulatory responses and reproductive traits in composite beef bulls raised in a tropical climate. International Journal of Biometeorology, 62(9), 1575–1586.

Staub, C., & Johnson, L. (2018). Review: Spermatogenesis in the bull. Animal, 12(s1), s27–s35.

Zhang, B. R., Larsson, B., Lundeheim, N., & Rodriguez-Martinez, H. (1998). Sperm characteristics and zona pellucida binding in relation to field fertility of frozen-thawed semen from dairy AI bulls. International Journal of Andrology, 21(4), 207–216.



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