Climatic adaptation of bovine subspecies monitored by seminal characteristics during winter months in tropical region / Adaptação climática das subespécies bovinas monitoradas pelas características seminais durante meses de inverno em região tropical

Ana Beatriz Marques de Almeida, Myrian Megumy Tsunokawa Hidalgo, Fábio Morotti, Maria Isabel Mello Martins

Abstract


The objective of this study was to evaluate parameters indicative of sperm quality (motility, vigor, and morphology) during the winter months in South-Mato Grosso, from Bos taurus and Bos indicus bulls, to analyze the climate influences in seminal changes. The ejaculate collects were performed on alternate days, using an artificial vagina, at a central for semen collection and bovine reproduction in the Midwest region from Brazil. In total, 122 ejaculates were evaluated, from 10 bulls, six Bos indicus and four Bos taurus. The ejaculates were submitted to evaluation of motility and vigor in phase contrast microscopy, and the sperm morphology was analyzed in a humid chamber. Samples of semen that presented 60% of motility and score 3 in vigor analysis were selected for freezing. After thawing, the motility and vigor were again evaluated. The statistical analysis was performed using a generalized linear mixed model (GLIMMIX) (P ? 0.05). Fresh (P = 0.016) and post-thawing vigor (P = 0.014), as well as the proportion of minor defects were influenced by the climate (P = 0.001) over the months. Among the subspecies (indicus x taurus), differences were observed only for minor morphological defects (3.27% indicus x 2.6% taurus; P = 0.009). It was concluded that, independent of the subspecies, freezing decreases the seminal parameters evaluated. In addition, indicus animals presented a lower proportion of minor defects, and taurine bulls seemed to be better adapted to the winter, although the climate did not generate significant alterations in ejaculate quality.


Keywords


bulls, cryopreservation, semen, sperm motility.

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References


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DOI: https://doi.org/10.34117/bjdv6n1-052

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