Production of microbial carotenoids by Rhodotorula lactosa isolated in the Brazilian tropical savanna / Produção de carotenoides microbianos por Rhodotorula lactosa isolada na savana tropical brasileira

Whallans Raphael Couto Machado, Vanildo Luiz Del Bianchi


Exploration of new biomes to obtain microorganisms of industrial interest can reveal a number of new strains but they are not all equally efficient in their carotenoids production under different environmental conditions. Screening is needed to select the elite strain and thus commercially produce the pigment. The main objective of this study was to increase the carotenoid production capacity of a recently isolated yeast obtained from the Cerrado (Brazilian tropical savanna) biome. Four hundred and seventy yeast colonies were isolated, of which 25 were pigment-producing yeasts. The promising yeast was identified, by molecular analysis (polymerase chain reaction - PCR) as Rhodotorula lactose, presenting 4.0 g/L of biomass and 776.67 µg/L (194.17 µg/g) of carotenoids (volumetric and specific) in the commercial medium. In the optimization, the carbon source (43 g/L of glucose) was the most significant and positive factor in the carotenoid production process (1318.27 µg/L and 145.58 µg/g), followed by the concentration of nitrogen sources (0.7 g/L yeast extract and 0.7 g/L peptone). The equation generated from the optimized process was validated and showed similar values in the carotenoid production (1233.80 µg/L and 156.60 µg/g) proving it to be promising process model for a scale-up using submerged culture.


Pigmented yeast, Factorial planning, Yeast screening, Orange yeast.


Poliak, P.; Škorňa, P.; Klein, E.; Lukeš, V. Thermodynamics of radical scavenging of symmetric carotenoids and their charged species. Food Chem. 2018; 268, 542– 549.

BCC Research. The global market for carotenoids. BCC Research: The global market for carotenoids. 2018. 141-146, (accessed JAN. 2021).

Pérez-Ibarbia, L.; Majdanski, T.; Schubert, S.; Windhab, N.; Schubert, U.S. Safety and regulatory review of dyes commonly used as excipients in pharmaceutical and nutraceutical applications. Eur J Pharm Sci. 2016, 93, 264–273,

Irías-Mata, A.; Jiménez, V.M.; Steingass, C.B.; Schweiggert, R.M.; Carle, R.; Esquivel, P. Carotenoids and xanthophyll esters of yellow and red nance fruits (Byrsonima crassifolia (L.) Kunth) from Costa Rica. Food Res Int. 2018, 111, 708–714,

Kot, A.M.; Błażejak, S.; Kurcz, A.; Gientka, I.; Kieliszek, M. Rhodotorula glutinis—potential source of lipids, carotenoids, and enzymes for use in industries. Appl Microbiol Biotechnol. 2016, 100, 6103– 6117,

Lopes, N.A.; Remedi, R.D.; Sá, C.S.; Burkert, C.A.V.; Burkert, J.F.M. Different cell disruption methods for obtaining carotenoids by Sporodiobolus pararoseus and Rhodothorula mucilaginosa. Food Sci Biotechnol, 2017, 26, 759–766,

Machado, W.R.C.; Burkert, J.F.M. Optimization of agroindustrial medium for the production of carotenoids by wild yeast Sporidiobolus pararoseus. African J Microbiol Res, 2015, 9, 209–219,

Irazusta, V.; Nieto-Peñalver, C.G.; Cabral, M.E.; Amoroso, M.J.; Figueroa, L.I.C. Relationship among carotenoid production, copper bioremediation and oxidative stress in Rhodotorula mucilaginosa RCL-11. Process Biochem, 2013, 48, 803–809,

Moreira, G.A.M.; Sperandio, E.M.; Vale, H.M.M. Yeasts associated to native plant fruits of Cerrado: Eugenia lutescens Cambess, Campomanesia xanthocarpa (Mart.) O. Berg and Brosimum guadichaudii Tréc. Rev Biol Neotrop, 2016, 12, 104-111.

Villarreal, P.; Carrasco, M.; Barahona, S.; Alcaíno, J.; Cifuentes, V.; Baeza, M. Tolerance to ultraviolet radiation of psychrotolerant yeasts and analysis of their carotenoid, mycosporine, and ergosterol content. Curr Microbiol, 2016, 72, 94–101.

Libkind, D.; Moliné, M.; Trochine, A.; Bellora, N.; Garcia, V. Biotechnologically relevant yeasts from patagonian natural environments. In: Biology and Biotechnology of Patagonian Microorganisms, Cham: Springer International Publishing, 2016, 325–351,

Husseiny, S.M.; Abdelhafez, A.A.; Ali, A.A.-A.; Sand, H.M. Optimization of β-carotene production from Rhodotorula glutinis ATCC 4054 growing on agro-industrial substrate using Plackett–Burman design. Proc Natl Acad Sci India Sect B Biol Sci, 2017, 88, 1637–1646,

Cabral, M.M.S.; Cence, K.; Zeni, J.; Tsai, S.M.; Durrer, A.; Foltran, L.L.; Luccio, M.D.; Oliveira, J.V.; Oliveira, D.; Treichel, H. Carotenoids production from a newly isolated Sporidiobolus pararoseus strain by submerged fermentation. Eur Food Res Technol, 2011, 233, 159–166.

Valduga, E.; Valério, A.; Treichel, H.; Luccio, M.D.; Jacques, R.A.; Fúrigo, J.A. Pretreatment of sugarcane molasses and corn steep liquor for the production of carotenoids. Quim Nova, 2007, 30, 1860–1866,

Pereira, G.V.M.; Soccol, V.T.; Pandey, A.; Medeiros, A.B.P.; Lara, J.M.R.A.; Gollo, A.L.; Soccol, C.R. Isolation, selection and evaluation of yeasts for use in fermentation of coffee beans by the wet process. Int J Food Microbiol, 2014, 188, 60–66,

Fonseca, R.A.S.; Rafael, R.D.S.; Kalil, S.J.; Burkert, C.A.V.; Burkert, J.F.M. Different cell disruption methods for astaxanthin recovery by Phaffia rhodozyma. African J Biotechnol, 2011, 10, 1165–1171,

Machado, W.R.C.; Silva, L.G., Vanzela, E.S.L.; Bianchi, V.L.D. Evaluation of the process conditions for the production of microbial carotenoids by the recently isolated Rhodotorula mucilaginosa URM 7409. Brazilian J Food Technol, 2019, 22, 1–14,

Machado, W.R.M.; Silva, L.G.; Vanzela, E.S.L.; Bianchi, V.L.D. Production of carotenoids by Rhodotorula toruloides isolated from Brazilian tropical savannah. Int Food Res J, 2019, 26, 1259–1267.

AOAC. Official Methods of Analysis of AOAC International (19th ed.). 19th ed. George Latimer J, editor. USA: Association of Official Analysis Chemists International.; 2012.

Miller, G.L. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem, 1959, 31, 426–428,

Chang C.-F.; Lee, C.-F.; Lin, K.-Y.; Liu, S.-M. Diversity of yeasts associated with the sea surface microlayer and underlying water along the northern coast of Taiwan. Res Microbiol, 2016, 167, 35–45.

Aksu, Z,; Eren, A.T. Production of carotenoids by the isolated yeast of Rhodotorula glutinis. Biochem Eng J, 2007, 35, 107–113,

Spier, F.; Buffon, J.G.; Burkert, C.A.V. Bioconversion of raw glycerol generated from the synthesis of biodiesel by different oleaginous yeasts: Lipid content and fatty acid profile of biomass. Indian J Microbiol, 2015, 55, 415–422,

Obruca, S.; Benesova, P.; Kucera, D.; Petrik, S.; Marova, I. Biotechnological conversion of spent coffee grounds into polyhydroxyalkanoates and carotenoids. N Biotechnol, 2015, 32, 569–574,

Schneider, T.; Graeff-Hönninger, S.; French, W.T.; Hernandez, R.; Merkt, N.; Claupein, W.; Hetrick, M.; Pham, P. Lipid and carotenoid production by oleaginous red yeast Rhodotorula glutinis cultivated on brewery effluents. Energy, 2013, 61, 34–43,

Cheng, Y.-T.; Yang, C.-F. Using strain Rhodotorula mucilaginosa to produce carotenoids using food wastes. J Taiwan Inst Chem Eng, 2016, 61, 270–275.

Braunwald, T.; Schwemmlein, L.; Graeff-Hönninger, S.; French, W.T.; Hernandez, R.; Holmes, W.E.; Claupein, W. Effect of different C/N ratios on carotenoid and lipid production by Rhodotorula glutinis. Appl Microbiol Biotechnol, 2013, 97, 6581– 6588.

Tkáčová, J.; Čaplová, J.; Klempová, T.; Čertík, M. Correlation between lipid and carotenoid synthesis in torularhodin-producing Rhodotorula glutinis. Ann Microbiol, 2017, 67, 541–551.

Luo, H.; Niu, Y.; Duan, C.; Su, H.; Yan, G. A pH control strategy for increased β-carotene production during batch fermentation by recombinant industrial wine yeast. Process Biochem, 2013, 48, 195–200.

Maldonade, I.R.; Rodriguez-Amaya, D.B.; Scamparini, A.R.P. Carotenoids of yeasts isolated from the Brazilian ecosystem. Food Chem, 2008, 107, 145–150,

Chauhan, B.; Gupta, R. Application of statistical experimental design for optimization of alkaline protease production from Bacillus sp. RGR-14. Process Biochem, 2004, 39, 2115–2122,

Tkáčová, J.; Klempová, T.; Čertík, M. Kinetic study of growth, lipid and carotenoid formation in β-carotene producing Rhodotorula glutinis. Chem Pap. 2018, 72, 1193–1203.

Otero, D.M.; Bulsing, B.A.; Huerta, K.M.; Rosa, C.A.; Zambiazi, R.C.; Burkert, C.A.V.; Burkert, J.F.M. Carotenoid-producing yeasts in the brazilian biodiversity: isolation, identification and cultivation in agroindustrial waste. Brazilian J Chem Eng, 2019, 36, 117–129,

Naghavi, F.S.; Hanachi, P.; Soudi, M.R.; Saboora, A.; Ghorbani, A. Evaluation of the relationship between the incubation time and carotenoid production in Rhodotorula Slooffiae and R. Mucilaginosa isolated from leather tanning. Iran J Basic Med Sci, 2013, 16, 1114– 1118.

Shichang, L.; Pengpeng, Z.; Shaobin, G.; Hongxia, L.; Ya, L.; Shengnan, L. Screening of lipid high producing mutant from Rhodotorula glutinis by low ion implantation and study on optimization of fermentation medium. Indian J Microbiol, 2013, 53, 343–351,



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