Adequate conditions of environmental management: lack of pathogens in community vegetables / Condições adequadas de manejo ambiental: ausência de patógenos em hortas comunitárias

Denise Junqueira Matos, Fernanda Pinto Ferreira, Natália Ingrid Pezzoti, Luana de Melo Scardovelli, Italmar Teodorico Navarro, Leticia Santos Balbino, Gabrieli Dutra Gonçalves, Katia Denise Saraiva Bresciani

Abstract


The hygiene and quality of raw food are of concern, as they can transmit bacteria, such as thermotolerant coliforms, protozoa such as Cryptosporidium spp., Giardia spp., Toxoplasma gondii and helminths of public health importance. Thus, in this work, the aim was to investigate environmental management conditions and the occurrence of Escherichia coli in irrigation water, helminths in vegetables, and protozoa in soil, fertilizer, water and vegetables from community gardens in Araçatuba-SP. For this, an epidemiological questionnaire was applied, in the form of an interview to workers and collected from each garden of the six registered with the Agro-industrial Development Secretariat of the City of Araçatuba, 40 liters of treated water or 10 liters of untreated water, a sample of approximately 10 g of soil and 10 g of fertilizer and randomly, a clump of lettuce and one of almond. Microbiological analyzes for the detection of E. coli using the Colilert Kit (IDEXX Laboratories, USA) were performed in irrigation waters. To search for helminths and their eggs in vegetables, it was performed by optical microscopy. The analysis of Cryptosporidium spp., Giardia spp. and T. gondii, was carried out by polymerase chain reaction (PCR) in samples of irrigation water, soil, fertilizer and vegetables. The location in an urban area, use of treated water, absence of pits, limitations of areas by walls, fences or fences, absence of domestic animals in community gardens, were sufficient to prevent E. coli in irrigation water, helminths in vegetables and Cryptosporidium spp., Giardia spp. and T. gondii, in samples of water, soil, fertilizer, and vegetables.


Keywords


Epidemiology, Enteroparasites, Environmental contamination, Fresh foods, Public Health, Vegetables.

References


AGUIRRE, A. A. et al. The One Health Approach to Toxoplasmosis: Epidemiology, Control, and Prevention Strategies. EcoHealth, v. 16, n. 2, p. 378–390, 15 jun. 2019. Disponível em: . Acesso em: 14 jul. 2021.

AHMED, I. et al. Microbiome, Metabolome and Inflammatory Bowel Disease. Microorganisms 2016, Vol. 4, Page 20, v. 4, n. 2, p. 20, 15 jun. 2016. Disponível em: . Acesso em: 27 jul. 2021.

ALLENDE, A.; MONAGHAN, J. Irrigation Water Quality for Leafy Crops: A Perspective of Risks and Potential Solutions. International Journal of Environmental Research and Public Health 2015, Vol. 12, Pages 7457-7477, v. 12, n. 7, p. 7457–7477, 3 jul. 2015. Disponível em: . Acesso em: 27 jul. 2021.

AMAHMID, O.; ASMAMA, S.; BOUHOUM, K. The effect of waste water reuse in irrigation on the contamination level of food crops by Giardia cysts and Ascaris eggs. International Journal of Food Microbiology, v. 49, n. 1–2, p. 19–26, 1 ago. 1999.Disponível em: . Acesso em: 9 jun. 2021.

AMOAH, P.; DRECHSEL, P.; ABAIDOO, R. C. Irrigated urban vegetable production in Ghana: Sources of pathogen contamination and health risk elimination. Irrigation and Drainage, v. 54, n. SUPPL. 1, p. S49–S61, 2005.

ANTUNES, L. et al. Parasitos em hortaliças comercializadas no sul do Rio Grande do Sul, Brasil. Revista de Ciências Médicas e Biológicas, v. 12, n. 1, p. 45, 21 ago. 2013. Disponível em: . Acesso em: 9 jun. 2021.

BARBOSA, F. R.; SILVA, C. S. B. da; CARVALHO, G. K. de L. Uso de inseticidas alternativos no controle de pragas agrícolas. - Portal Embrapa. Petrolina: Embrapa Semi-Árido, 2006.

BRANCO, N.; LEAL, D. A. G.; FRANCO, R. M. B. A Parasitological Survey of Natural Water Springs and Inhabitants of a Tourist City in Southeastern Brazil. https://home.liebertpub.com/vbz, v. 12, n. 5, p. 410–417, 11 maio 2012. Disponível em: . Acesso em: 27 jul. 2021.

BRASIL. Ministério da Saúde, Fundação Nacional de Saúde, Departamento de Saúde Ambiental, Coordenação de Controle da Qualidade da Água. Manual prático de análise de água. 4. ed. Brasília: FUNASA; 2013.

CHAIDEZ, C. et al. Occurrence of Cryptosporidium and Giardia in irrigation water and its impact on the fresh produce industry. International Journal of Environmental Health Research, v. 15, n. 5, p. 339–345, out. 2005. Disponível em: . Acesso em: 9 jun. 2021.

COELHO, L. M. et al. Detection of transmissible forms of enteroparasites in water and vegetables consumed at schools in Sorocaba, São Paulo state, Brazil. Revista da Sociedade Brasileira de Medicina Tropical, v. 34, n. 5, p. 479–482, 2001.

COKLIN, T. et al. Prevalence and molecular characterization of Giardia duodenalis and Cryptosporidium spp. in dairy cattle in Ontario, Canada. Veterinary Parasitology, v. 150, n. 4, p. 297–305, 25 dez. 2007.

COOLEY, M. B. et al. Development of a Robust Method for Isolation of Shiga Toxin-Positive Escherichia Coli (STEC) from Fecal, Plant, Soil and Water Samples from a Leafy Greens Production Region in California. PloS one, v. 8, n. 6, p. e65716, 2013.

DIXON, B. R. Parasitic illnesses associated with the consumption of fresh produce - an emerging issue in developed countriesCurrent Opinion in Food ScienceElsevier Ltd, , 1 abr. 2016. .

EDBERG, S. C. et al. Escherichia Coli: The Best Biological Drinking Water Indicator for Public Health Protection. Symposium series (Society for Applied Microbiology), n. 29, p. 106S-116S, 2000.

EFSTRATIOU, A.; ONGERTH, J. E.; KARANIS, P. Waterborne transmission of protozoan parasites: Review of worldwide outbreaks - An update 2011–2016. Water Research, v. 114, p. 14–22, 2017. Disponível em: .

FAO/WHO (Food and Agriculture Organization of the United Nations and World Health Organization) Multicriteria-Based Ranking for Risk Management of Food-Borne Parasites. 2012.

FERREIRA, F. P. et al. The effect of water source and soil supplementation on parasite contamination in organic vegetable gardens. Revista Brasileira de Parasitologia Veterinaria, v. 27, n. 3, p. 327–337, 1 jul. 2018. Disponível em: . Acesso em: 9 jun. 2021.

GAD, M. A. et al. Prevalence of enteric protozoa on fresh vegetables and their irrigation water in Giza, Egypt. Egyptian Journal of Aquatic Biology and Fisheries, v. 24, n. 2, p. 451–468, 2020.

GAO, X. et al. Land use and soil contamination with Toxoplasma gondii oocysts in urban areas. Science of The Total Environment, v. 568, p. 1086–1091, 2016. Disponível em: .

GUPTA, N.; KHAN, D. K.; SANTRA, S. C. Prevalence of intestinal helminth eggs on vegetables grown in wastewater-irrigated areas of Titagarh, West Bengal, India. Food Control, v. 20, n. 10, p. 942–945, 1 out. 2009.

HENZ, G. P.; DE ALCÂNTARA, F. A. Hortas: o produtor pergunta, a Embrapa responde. - Portal Embrapa. Disponível em: . Acesso em: 17 maio. 2021.

JAY, M. T. et al. Escherichia Coli O157:H7 in Feral Swine near Spinach Fields and Cattle, Central California Coast. Emerging infectious diseases, v. 13, n. 12, p. 1908–1911, dez. 2007.

JEFFERS, V. et al. A latent ability to persist: differentiation in Toxoplasma gondii. Cellular and Molecular Life Sciences 2018 75:13, v. 75, n. 13, p. 2355–2373, 30 mar. 2018. Disponível em: . Acesso em: 14 jul. 2021.

LASS, A. et al. The first detection of Toxoplasma gondii DNA in environmental fruits and vegetables samples. European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, v. 31, n. 6, p. 1101–1108, jun. 2012. Disponível em: . Acesso em: 14 jul. 2021.

LUNA-GUEVARA, J. J. et al. The Role of Pathogenic E. Coli in Fresh Vegetables: Behavior, Contamination Factors, and Preventive Measures. International Journal of Microbiology, v. 2019, 2019.

MOYNE, A. et al. Conditions at the time of inoculation influence survival of attenuated Escherichia coli O157:H7 on field-inoculated lettuce. Food Microbiology, v. 85, p. 103274, 2020. Disponível em: .

ORGANIZAÇÃO PAN AMERICANA DE SAÚDE. (2001). Água e Saúde. Disponível em: https://www.paho.org/bra/index.php?option=com_docman&view=download&alias=203-agua-e-saude-3&category_slug=saude-e-ambiente-707&Itemid=965 . Acesso em: 01/01/2020

PERES, F. et al. AGROTÓXICOS, SAÚDE E AMBIENTE: uma introdução ao tema. 2003.

PINTO-FERREIRA, F. et al. Patterns of Transmission and Sources of Infection in Outbreaks of Human Toxoplasmosis. Emerging Infectious Diseases, v. 25, n. 12, p. 2177–2182, dez. 2019. Disponível em: .

PINTO-FERREIRA, F. et al. Molecular diagnosis of the curly lettuce parasitic contamination from hydroponic cultivation from supermarkets. Revista Brasileira de Parasitologia Veterinaria, v. 29, n. 4, p. 1–5, 20 nov. 2020. Disponível em: . Acesso em: 9 jun. 2021.

QURESHI, A. S. et al. Evaluating heavy metal accumulation and potential health risks in vegetables irrigated with treated wastewater. Chemosphere, v. 163, p. 54–61, 2016.

RAFAEL, K. et al. Genotyping of Giardia duodenalis in vegetables cultivated with organic and chemical fertilizer from street markets and community vegetable gardens in a region of Southern Brazil. Transactions of the Royal Society of Tropical Medicine and Hygiene, v. 111, n. 12, p. 540–545, 1 dez. 2017. Disponível em: . Acesso em: 17 maio. 2021.

ROSE, J. B.; HUFFMAN, D. E.; GENNACCARO, A. Risk and control of waterborne cryptosporidiosis. FEMS Microbiology Reviews, v. 26, n. 2, p. 113–123, 1 jun. 2002. Disponível em: . Acesso em: 27 jul. 2021.

SPILKI, F. R. et al. Detection of human adenovirus, rotavirus and enterovirus in water samples collected on dairy farms from Tenente Portela, Northwest of Rio Grande do Sul, Brazil. Brazilian Journal of Microbiology, v. 44, n. 3, p. 953–957, jul. 2013. Disponível em: . Acesso em: 10 ago. 2021.

THOMPSON, R. C. A.; ASH, A. Molecular epidemiology of Giardia and Cryptosporidium infections. Infection, Genetics and Evolution, v. 40, p. 315–323, 2016. Disponível em: .

TOLEDO, R. dos S. et al. Cryptosporidium spp. and Giardia spp. in feces and water and the associated exposure factors on dairy farms. PLOS ONE, v. 12, n. 4, p. e0175311, 1 abr. 2017. Disponível em: . Acesso em: 10 ago. 2021.

TRABULSI, L. R.; KELLER, R.; GOMES, T. A. T. Typical and Atypical Enteropathogenic Escherichia coli - Volume 8, Number 5—May 2002 - Emerging Infectious Diseases journal - CDC. Emerging Infectious Diseases, v. 8, n. 5, p. 508–513, 2002. Disponível em: . Acesso em: 27 jul. 2021.

USEPA (1986) Ambient Water Quality Criteria for Bacteria . Washington, DC : USEPA

WADAMORI, Y.; GOONERATNE, R.; HUSSAIN, M. A. Outbreaks and factors influencing microbiological contamination of fresh produce. Journal of the Science of Food and Agriculture, v. 97, n. 5, p. 1396–1403, 1 mar. 2017. Disponível em: . Acesso em: 27 jul. 2021.

WEISS, L. M.; DUBEY, J. P. Toxoplasmosis: a history of clinical observations. International journal for parasitology, v. 39, n. 8, p. 895, 1 jul. 2009. Disponível em: /articles/PMC2704023/>. Acesso em: 14 jul. 2021.




DOI: https://doi.org/10.34117/bjdv7n9-166

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