Adequate conditions of environmental management: lack of pathogens in community vegetables / Condições adequadas de manejo ambiental: ausência de patógenos em hortas comunitárias

Denise Junqueira Matos, Fernanda Pinto Ferreira, Natália Ingrid Pezzoti, Luana de Melo Scardovelli, Italmar Teodorico Navarro, Leticia Santos Balbino, Gabrieli Dutra Gonçalves, Katia Denise Saraiva Bresciani


The hygiene and quality of raw food are of concern, as they can transmit bacteria, such as thermotolerant coliforms, protozoa such as Cryptosporidium spp., Giardia spp., Toxoplasma gondii and helminths of public health importance. Thus, in this work, the aim was to investigate environmental management conditions and the occurrence of Escherichia coli in irrigation water, helminths in vegetables, and protozoa in soil, fertilizer, water and vegetables from community gardens in Araçatuba-SP. For this, an epidemiological questionnaire was applied, in the form of an interview to workers and collected from each garden of the six registered with the Agro-industrial Development Secretariat of the City of Araçatuba, 40 liters of treated water or 10 liters of untreated water, a sample of approximately 10 g of soil and 10 g of fertilizer and randomly, a clump of lettuce and one of almond. Microbiological analyzes for the detection of E. coli using the Colilert Kit (IDEXX Laboratories, USA) were performed in irrigation waters. To search for helminths and their eggs in vegetables, it was performed by optical microscopy. The analysis of Cryptosporidium spp., Giardia spp. and T. gondii, was carried out by polymerase chain reaction (PCR) in samples of irrigation water, soil, fertilizer and vegetables. The location in an urban area, use of treated water, absence of pits, limitations of areas by walls, fences or fences, absence of domestic animals in community gardens, were sufficient to prevent E. coli in irrigation water, helminths in vegetables and Cryptosporidium spp., Giardia spp. and T. gondii, in samples of water, soil, fertilizer, and vegetables.


Epidemiology, Enteroparasites, Environmental contamination, Fresh foods, Public Health, Vegetables.


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