Resistance Profile of P. aeruginosa isolates to β-lactams antimicrobials/ Perfil de resistência da P. aeruginosa isola para β-lactams antimicrobianos

Adailton Pereira dos Santos, Andressa Liberal Santos, Camila Zanatta de Oliveira, Célia Regina Malveste Ito, Giselle Pinheiro Lima Aires Gomes, Adenícia Custódia Silva e Souza, Lara Stefânia Netto de Oliveira Leão Vasconcelos, Mônica de Oliveira Santos, Lilian Carla Carneiro

Abstract


β-lactamases are enzymes that hydrolyze the β-lactam ring, inactivating the action of β-lactam antibiotics. The objective of this work was to diagnose phenotypically and molecularly 14 β-lactamase resistance genes expressed in Pseudomonas aeruginosa and to correlate the results found. A total of 99 samples of Pseudomonas aeruginosa were selected and the antibiogram was performed. Real-time PCR is being performed using the Sybr Green system to amplify the genes corresponding to the resistances found in phenotyping. Of the 99 samples, 14 were identified as phenotypically resistant to the antimicrobial Aztreonam. The blaSME, blaOXA, blaGIM genes were simultaneously found in three (21.4%) samples. According to the statistical test, when evaluating the amplification results obtained for piperacillin + tazobactam, the molecular method was more sensitive for the detection of the gene coding for multidrug resistance, presenting values of (p <0.05). This information suggests gene researches are more sensitive and specific when compared with to the antibiogram test.


Keywords


Antibiogram, Antimicrobial Resistance, β-lactams, Genes, Molecular Diagnosis.

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References


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DOI: https://doi.org/10.34117/bjdv7n7-278

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