Short Communication: Antibacterial activity of Cefquinome Sulfate in the presence of biofilm Staphylococcus spp isolated from bovine milk / Comunicação breve: Actividade antibacteriana do sulfato de Cefquinome na presença do biofilme Staphylococcus spp isolado do leite bovino

Stéfani Thais Alves Dantas, Bruna Fernanda Rossi, Caroline Faganello, Ivana Giovannetti Castilho, Gousilin Leandra Rocha da Silva, Juliano Leonel Gonçalves, Marcos Veiga dos Santos, Erika Carolina Romão Bonsaglia

Abstract


Bovine mastitis is an inflammation of the mammary gland in dairy cattle usually caused by bacterial agents and cefquinome is a fourth-generation cephalosporin commonly used in treatment of this disease caused by Staphylococcus spp. in lactating cows due to a broad spectrum. On the other hand, biofilm formation provides protection for bacteria increasing the resistance to antibiotics and contributing to the persistence of infection. The objective of the present study was to assess if biofilm produced by Staphylococcus spp was able to protect the bacteria from cefquinome sulfate (CFQ) action for 24h exposure. Our findings showed that 95% (n=53/56) of Staphylococcus spp. were biofilm producer and the biofilm protected the most of species tested, except S. xylosus maybe due to the formation of a thin layer biofilm that did not interfere in the antimicrobial action. Thus, the effectiveness of cefquinome in persistent infections may be compromised due to a thicker biofilm.


Keywords


Biofilm, Mastitis, Resistance, Staphylococcus spp.

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References


Barcelos MM, Martins L, Grenfell RC, Juliano L, Anderson KL, Dos Santos MV, Gonçalves JL. Comparison of standard and on-plate extraction protocols for identification of mastitis-causing bacteria by MALDI-TOF MS. Braz J Microbiol. 2019; 50(3): 849-857. https://doi.org/10.1007/s42770-019-00110-5.

CLSI (Clinical Laboratory Standards Institute) Performance Standards for Antimicrobial Susceptibility Testing. Twenty-Second Informational Supplement M100-s22, National Committee for Clinical Laboratory Standards, Wayne, PA. 2018.

Felipe MCA, Somale PS, Varroni F, Zingaretti ML, Bachetti RA, Correa SG, Porporatto C. Evaluation of the biofilm forming ability and its associated genes in Staphylococcus species isolates from bovine mastitis in Argentinean dairy farms. Microb Pathog. 2017; 104:278-286. https://doi.org/10.1016/j.micpath.2017.01.047.

Felipe V, Bresser ML, Bohl LP, Silva ER, Morgante CA, Correa SG, Porporatto C. Chitosan disrupts biofilm formation and promotes biofilm eradication in Staphylococcus species isolated from bovine mastitis. Int J Biol Macromol. 2019; 126:60-67. https://doi.org/10.1016/j.ijbiomac.

Goetz C, Tremblay YDN, Lamarche D, Blondeau A, Gaudreau AM, Labrie J, Malouin F, Jacques M. Coagulase-negative staphylococci species affect biofilm formation of other coagulase-negative and coagulase-positive staphylococci. J Dairy Sci. 2017; 100:1–11. https://doi.org/10.3168/jds.2017-12629.

Isaac P, Bohl LP, Breser ML, Orellano MS, Conesa A, Ferrero MA, Porporatto C. Commensal coagulase-negative Staphylococcus from the udder of healthy cows inhibits biofilm formation of mastitis-related pathogens. Vet Microbiol. 2017; 207:259-266. https://doi.org/10.1016/j.vetmic.2017.05.025.

Okuda KI, Nagahori R, Yamada S, Sugimoto S, Sato C, Sato M, Mizunoe Y. The Composition and Structure of Biofilms Developed by Propionibacterium acnes Isolated from Cardiac Pacemaker Devices. Front Microbiol. 2018; 9:182. https://doi.org/10.3389/fmicb.2018.00182.

Stepanovic S, Vukovic D, Dakic I, Savic B, Svabic-Vlahovic M. A modified microtiter-plate test for quantification of staphylococcal biofilm formation. J Microbiol Methods. 2000; 40(2):175-179. https://doi.org/10.1016/S0167-7012(00)00122-6.

Stepanovic S, Vukovic D, Hola V, Bonaventura GD, Djukic S, CIrkovic I, Ruzicka F. Quantification of biofilm in microtiter plates: Overview of testing conditions and practical recommendations for assessment of biofilm production by staphylococci. Acta: Pathol Microbiol Immunol Scand Sect. 2007; 115(8):891-899. https://doi.org/10.1111/j.1600-0463.2007.apm_630.x.

Tremblay DN, Lamarche D, Chever P, Haine D, Messier S, Jacques M. Characterization of the ability of coagulase-negative staphylococci isolated from the milk of Canadian farms to form biofilms. J Dairy Sci. 2013; 96 (1):243-246. https://doi.org/10.3168/jds.2012-5795.

Zonca A, Gallo M, Locatelli C, Carli S, Moroni P, Villa R, Cagnardi P. Cefquinome sulfate behavior after intramammary administration in healthy and infected cows. J Dairy Sci. 2011; 94(7):3455-61. https://doi.org/10.3168/jds.2010-4109.




DOI: https://doi.org/10.34117/bjdv7n7-144

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