Efeito do óleo essencial de Ocimum basilicum L. contra isolados clínicos de Klebsiella spp. produtores de carbapenemases (KPC)/ Effect of Ocimum basilicum L. essential oil against clinical isolates of Klebsiella spp. carbapenemase producers (KPC)

Beatriz Bohns Pruski, Suelen Cavalheiro Amaral, Marcelle de Oliveira Garcia, Kamila Furtado da Cunha, Silvia de Oliveira Hübner, Daiane Drawanz Hartwig


O controle de bactérias como Klebsiella pneumoniae produtora de carbapenemases (KPC) é bastante difícil e a busca por novos antibacterianos necessária. Assim, o objetivo deste estudo foi avaliar a capacidade antibacteriana do óleo essencial de Ocimum basilicum L. (OEM), como alternativa terapêutica no controle de KPC e sua citotoxicidade em células de mamífero. Dessa forma, foram avaliados 48 isolados clínicos de Klebsiella spp. confirmados como K. pneumoniae através do gene dnaA, e avaliados quanto a produção de carbapenemase (gene blaKPC e teste de Hogde). O perfil de sensibilidade dos isolados foi avaliado frente a treze diferentes antimicrobianos. O OE foi testado em concentrações variando de 1,8 a 306,6 mg.mL-1 para determinar a concentração inibitória mínima (CIM) e a concentração bactericida mínima (CBM), frente aos isolados de K. pneumoniae. Dos 48 isolados, 45 foram confirmados como K. pneumoniae e 15 isolados como positivos para a presença do gene blaKPC e teste de Hodge para presença da enzima carbapenemase. No teste de difusão em disco os antibacterianos meropenem e imipenem demonstraram o menor grau de resistência, enquanto as classes cefalosporinas e betalactâmicos apresentaram alta resistência. A CIM foi observada em concentrações variando de 3,6 a 131,4 mg.mL-1 nos isolados KPC, enquanto a CBM variou entre 3,6 a 306,6 mg.mL-1. O OEM demonstrou-se tóxico nas concentrações de CIM e CBM frente às células MDBK, contudo, alternativas como elaboração de nanoemulsões, podem diminuir sua citotoxicidade e potencializar sua ação antibacteriana. Os resultados obtidos são promissores e potenciais alternativas terapêuticas frente a este importante patógeno.


Klebsiella pneumoniae, carbapenemase, blaKPC, manjericão, resistência.


Alkhateed, M.A., Al-Otaibi, W.R., Algabbani, Q., Alsakran, A.A., Alnafjan, A.A., Alotaibi, A.M., Al-Qahtani, W.S. (2021) Low-temperature extrects of purple blossoms of basil (Ocimum basilicum L.) intevened mitcochondrial translocation contributes prompted apoptosis in human breast cancer cells. Biological Research, 6, 54 (1).

Almalki, D.A. (2019) Renoprotective effect of Ocimum basilicum (Basil) against diabetes-induced renal affection in Albino rats. Materia Socio-Medica, 31 (4), 236-240.

Andrade, B.F.M., Barbosa, L.N., Probst, I.S., Junior, A.F. (2014). Antimicrobial activity of essential oils, Journal of Essential Oil Research, 26, 34-40.

Bakkali, F., Averbeck, S., Averbeck, D., Idaomar M. (2008) Biological effects of essential oils–a review. Food. Chemical Toxicology, 46, 446-75.

Bassetti, M., Giacobbe, D.R., Giamarellou H., Viscoli, C., Daikos, G.L., Dimopoulos, G., de Rosa, F.G., Giamarellos-Bourboulis, E.J., Rossolini, G.M., Righi, E., Karaiskos, I., Tumbarello, M., Nicolau, D.P., Viale, P.L., Poulakou, G. (2018) Management of KPC-producing Klebsiella pneumoniae infections. Clinical Microbiology and Infection, 24, 133–144.

Bassetti, M., Peghin, M. (2020) How to manage KPC infections. Therapeutic Advances in Infectious Disease, 7, 1–12.

Burt, S. (2004) Essential oils: their antibacterial properties and potential applications in foods-a review. International Journal Food. Microbiology, l94, 223-53.

Campos, P.A., Royer, S., Bastião, D.W.F., Araújo, B.F., Queiroz, L.L., Brito, C.S., Gontijo-Filho, P.P., Ribas, R.M. (2016) Multidrug resistance related to biofilm formation in Acinetobacter baumannii and Klebsiella pneumoniae clinical strains from different pulsotypes. Current Microbiology, 72, 5, 617–627.

Cardoso, N. N. R., Alviano, C.S., Blank, A., Arrigoni-Blank, M. F., Romanos, M. T. V., Cunha, M. M. L., Silva, A. J., Alviano, D. S. (2017) Anti-cryptococcal activity of ethanol crude extract and hexane fraction from Ocimum basilicum var. Maria bonita: mechanism of action and synergism with amphotericin B and Ocimum basilicum essential oil. Pharmaceutical Biology, 55 (1), 1380-1388.

CLSI (2018). Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically; M07 Approved Standard — 13th Edition.

de Campos, P.A., Royer, S., Bastião, D.W.F., Araújo, B.F., Queiroz, L.L., Brito, C.S., Gontijo-Filho, P.P., Ribas, R.M. (2016) Multidrug resistance related to biofilm formation in Acinetobacter baumannii and Klebsiella pneumoniae clinical strains from different pulsotypes. Current Microbiology, 72, 5, 617–627.

El-Soud, N. H. A., Deabes, M., El-Kassem, L. A., Khallil, M. (2012) Chemical composition and Antifungal Activity of Ocimum basilicum L. essential oil. Macedonian Journal of Medical Sciences, 15, 3 (3), 374-379.

Feil, E. J. (2016). Enterobacteriaceae: joining the dots withpan-European epidemiology. Lancet Infection Diseases, 17, 118–119.

Gibbons, S. (2004). Anti-staphylococcal plant natural products. Natural Product Reports, 21(2), 263.

Giske, C.G., Gezeluis, L., Samuelsen, Ø., Warner, M., Sundsfjord, A., Woodford, N. (2011) A sensitive and specific phenotypic assay for detection of metallo-blactamases and KPC in Klebsiella pneumoniae with the use of meropenem disks supplemented with aminophenylboronic acid, dipicolinic acid and cloxacillin. Clinical Microbiology and Infection, 17, 4, 552–556.

Han, R., Shi, Q., Wu, S., Yin, D., Peng, M., Dong, D., Zheng, Y., Guo, Y., Zhang, R., Hu, F. and the China Antimicrobial Surveillance Network (CHINET) Study Group (2020) Dissemination of Carbapenemases (KPC, NDM,OXA-48, IMP, and VIM) Among Carbapenem-Resistant Enterobacteriaceae Isolated From Adult and Children Patients in China. Frontiers in Cellular and Infection Microbiology, 10, 314.

Hodge, W., Ciak, J., Tramont, E.C. (1978) Simple method for detection of penicilinase-producing Neisseria gonorrhoeae. Journal Clinical Microbiology, 7, 102-103.

Lee, C.-T., Hsiao, K.-M., Chen, J.-C., Su, C.-C. (2015) Multiplex polymerase chain reaction assay developed to diagnose adult bacterial meningitis in Taiwan. APMIS, 123, 11, 945-950.

Lin, C.Y., Chen, L.W. (2008) Comparison of fuel properties and emission characteristics of two-and three-phase emulsions prepared by ultrasonically vibrating and mechanically homogenizing emulsification methods. Fuel, 31, 87, 2154-61.

Markosian, A. A. A., Petrosyan, M., Sahakyan, N., Babayan, A., Aloyan, S., Trchounian, A. T. (2017) Chemical composition and some biological activities of the essential oils from basil Ocimum different cultivars. BCM Complementary and Alternative Medicine, 60, 17, 1-8.

McClements, D.J. (2012) Nanoemulsions versus microemulsions: terminology, differences, and similarities. Soft Matter,8, 1719-29.

Mosmann, T. (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of Immunological Methods, 65, 55-63.

Nair, R., Kalariya, T., Chanda, S. (2008). Antibacterial Activity of Some Plant Extracts Used in Folk Medicine. Journal of Herbal Pharmacotherapy, 7 (3-4), 191–201.

Nazzaro, F., Fratianni, F., de Martino, L., Coppola, R., de Feo, V. (2013) Effect of essential oils on pathogenic bacteria. Pharmaceuticals (Basel), 6, 1451-74.

Nordmann, P., Dortet, L., Poirel, L. (2012a) Carbapenem resistance in Enterobacteriaceae: here is the storm. Trends in Molecular Medicine, 18, 263–272.

Nordmann, P., Girlich, D., Poirel, L. (2012b) Detection of carbapenemase producers in Enterobacteriaceae using a novel screening medium. Journal Clinical Microbiology, 50, 8, 2761-2766.

Ostertag, F., Weiss, J., McClements, D.J. (2012) Low-energy formation of edible nanoemulsions: factors influencing droplet size produced by emulsion phase inversion. Journal of Colloid and Interface Science, 388, 95-102.

Ozdikmenli, S., Zorba, N. N. D. (2016) Evaluaton of usage of essential oils instead of spices in meat ball formulation for controlling Salmonella spp. Food Science and Technology International, 22, 2, 93-101.

Perez, F., and Villegas, M. V. (2015) The role of surveillance systemsin confronting the global crisis of antibiotic-resistant bacteria. Current Opinion in Infectious Diseases, 28, 375–383.

Picoli, T., Barbosa, J. S., Vargas, G. D’Á., Hübner, S. de O., Fischer, G. (2015) Toxicidade e eficiência do dimetilsulfóxido (DMSO) no congelamento de células Madin-Darby Bovine Kidney (MDBK). Science and Animal Health, 3, 2, 159-168.

Rabbani, M., Sajjadi, S. E., Vaezi, A. (2015) Evaluation of anxiolytic and sedative effect of essential oil and hydroalcoholic extract of Ocimum basilicum L. and chemical composition of its essential oil. Research in Pharmaceutical Sciences, 10, 6, 535-543.

Sakkas, H., Gousia, P., Economou, V., Sakkas, V., Petsios, S., Papadopoulou, C. (2016) In vitro antimicrobial activity of five essential oils on multidrug resistant Gram-negative clinical isolates. Journal of Intercultural Ethnopharmacology, 5, 212-218.

Shafiri, A., Mohammadzadeh, A., Salehi, Z., Mahmoodi, P. (2018) Antibacterial, antibiofilm and antiquorum sensing effects of Thymus daennsis and Satureja hortensis essential oils against Staphylococcus aureus isolates. Journal of Applied Microbiology, 122, 2, 379-388.

Sienkiewicz, M., Łysakowska, M., Kowalczyk, E., Szymańska, G., Kochan, E., Krukowska, J., Olszewski, J., Zielińska-Bliźniewska, H. (2017). The ability of selected plant essential oils to enhance the action of recommended antibiotics against pathogenic wound bacteria. Burns, 43, 2, 310–317.

Silva, V. A., Sousa, J. P., Pêssoa, H. L. F., Freitas, A. F. R., Coutinho, H. D. M., Alves, L. B. N., Lima, E. O. (2015) Ocimum basilicum: antibacterial activity and association study with antibiotics against bacterial of clinical importance. Pharmaceutical Biology, 54, 4, 863-7.

Snoussi, M., Dehmani, A., Noumi, E. Flamini, G. Papetti, A. (2016) Chemical composition and antibiofilm activity of Petroselinum crispum and Ocimum basilicum essential oils against Vibrio spp. strains. Microbial Pathogenesis, 90, 13-21.

Stavri, M., Piddock, L. J. V., Gibbons, S. (2007) Bacterial efflux pump inhibitors from natural sources. Journal of Antimicrobial Chemotherapy, 59(6), 1247–1260.

Sundararajan, B., Moola, A. K., Vivek, K., Kumari, B. D. R. (2018). Formulation of nanoemulsion from leaves essential oil of Ocimum basilicum L. and its antibacterial, antioxidant and larvicidal activities (Culex quinquefasciatus). Microbial Pathogenesis, 125, 475-485.

Tegos, G. P.; Hamblin, M. R. (2006). Phenothiazinium Antimicrobial Photosensitizers Are Substrates of Bacterial Multidrug Resistance Pumps. Antimicrobial Agents and Chemotherapy, 50, 1, 196–203.

Tran, G.M., Ho-Le, T.P., Ha, D.T., Tran-Nguyen, C.H., Nguyen, T.S.M., Pham, T.T.N., Nguyen, T.V., Hoang, H.Q., Tran, N.V., Nguyen, T.V. (2017) Patterns of antimicrobial resistance in intensive care unit patients: a study in Vietnam. BMC Infectious Diseases, 17, 1, 429.

Vasconcelos, N. G., Croda, J., Simionatto, S. (2018) Antibacterial mechanisms fo cinnamom and its constituents: A review. Microbial Pathogenesis, 120, 198-203.

WHO, World Health Organization (2018) Global priority list of antibiotic-resistant bacteria to guide research, discovery and development of new antibiotics.

Xu, L., Sun, X., Ma, X. (2017) Systematic review and meta-analysis of mortality of patients infected with carbapenem-resistant Klebsiella pneumoniae. Annals of Clinical Microbiology and Antimicrobials, 16, 18.

DOI: https://doi.org/10.34117/bjdv7n6-472


  • There are currently no refbacks.