Efeito do óleo essencial de Ocimum basilicum L. contra isolados clínicos de Klebsiella spp. produtores de carbapenemases (KPC)/ Effect of Ocimum basilicum L. essential oil against clinical isolates of Klebsiella spp. carbapenemase producers (KPC)

Beatriz Bohns Pruski, Suelen Cavalheiro Amaral, Marcelle de Oliveira Garcia, Kamila Furtado da Cunha, Silvia de Oliveira Hübner, Daiane Drawanz Hartwig

Abstract


O controle de bactérias como Klebsiella pneumoniae produtora de carbapenemases (KPC) é bastante difícil e a busca por novos antibacterianos necessária. Assim, o objetivo deste estudo foi avaliar a capacidade antibacteriana do óleo essencial de Ocimum basilicum L. (OEM), como alternativa terapêutica no controle de KPC e sua citotoxicidade em células de mamífero. Dessa forma, foram avaliados 48 isolados clínicos de Klebsiella spp. confirmados como K. pneumoniae através do gene dnaA, e avaliados quanto a produção de carbapenemase (gene blaKPC e teste de Hogde). O perfil de sensibilidade dos isolados foi avaliado frente a treze diferentes antimicrobianos. O OE foi testado em concentrações variando de 1,8 a 306,6 mg.mL-1 para determinar a concentração inibitória mínima (CIM) e a concentração bactericida mínima (CBM), frente aos isolados de K. pneumoniae. Dos 48 isolados, 45 foram confirmados como K. pneumoniae e 15 isolados como positivos para a presença do gene blaKPC e teste de Hodge para presença da enzima carbapenemase. No teste de difusão em disco os antibacterianos meropenem e imipenem demonstraram o menor grau de resistência, enquanto as classes cefalosporinas e betalactâmicos apresentaram alta resistência. A CIM foi observada em concentrações variando de 3,6 a 131,4 mg.mL-1 nos isolados KPC, enquanto a CBM variou entre 3,6 a 306,6 mg.mL-1. O OEM demonstrou-se tóxico nas concentrações de CIM e CBM frente às células MDBK, contudo, alternativas como elaboração de nanoemulsões, podem diminuir sua citotoxicidade e potencializar sua ação antibacteriana. Os resultados obtidos são promissores e potenciais alternativas terapêuticas frente a este importante patógeno.


Keywords


Klebsiella pneumoniae, carbapenemase, blaKPC, manjericão, resistência.

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DOI: https://doi.org/10.34117/bjdv7n6-472

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