Determination of amino acids and nutritional aspects in transgenic soybean exposed to glyphosate/ Determinação de aminoácidos e aspectos nutricionais na soja transgênica exposta ao glifosato

André Luiz de Souza Lacerda, João Nicanildo Bastos dos Santos, Ary Gertes Carneiro Junior, Rodrigo Anselmo Tarsitano, Ricardo Salvador da Silva

Abstract


With the increase of glyphosate tolerant transgenic soybean cultivation areas, this substance has become the main herbicide for this crop, its use may interfere in the metabolism and nutritional aspects of transgenic plants. In this scenario, the objective of this work was to verify the oil, protein, phytate and amino acid concentrations in glyphosate tolerant soybean cultivar. Comparing the averages between oil, protein and total amino acid levels in transgenic soybean exposed and not exposed to glyphosate, it was found that there was no significant difference by Tukey test at 5% probability. In conclusion, the data indicate that although the transgenic event affects some routes, amino acid synthesis was not affected nor in nutritional terms at the glyphosate doses studied.


Keywords


soybean, phytate, amino acids, transgenics, glyphosate.

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References


ARRUDA, S.C.C.; BARBOSA, H.S.; AZEVEDO, R.A.; ARRUDA, M.A.Z. Comparative studies focusing on transgenic through cp4EPSPS gene and non-transgenic soybean plants: An analysis of protein species and enzymes. Journal of Proteomics, v.93, p.107-116, 2013.

BARBOSA, H.S.; ARRUDA, S.C.C.; AZEVEDO, R.A.; ARRUDA, M.A.Z. New insights on proteomics of transgenic soybean seeds: evaluation of differential expressions of enzymes and proteins. Anal Bioanal Chemistry, v.402, p.299–314, 2012.

BIELESKI, R.L.; TURNER, N.A. Separation and estimation of amino acids in crude plant extracts by thin-layer electrophoresis and chromatography. Analytical Biochemistry, v.17, p.278-293, 1996.

BONATO, R.E; BERTAGNOLLI, P.F.; LANGE, C.E.; RUBIN, S.A.L.R. Teor de óleo e de proteína em genótipos de soja desenvolvidos após 1990. Pesquisa Agropecuária Brasileira, v.35, p.2391-2398, 2000.

FEHR, W.E.; CAVINESS, C.E.; BURMOOD, D.T.; PENNINGTON, J.S. Stage of development descriptons for soybeans. Glycine max (L.) Merrill. Crop Science, v.11, p.929-31, 1971.

FRANCO, D.A.S.; ALMEIDA, S.D.B.; CERDEIRA, A.L.; DUKE, S.O.; MORAES, R.M.; LACERDA, A.L.S.; MATALLO, M.B. Evaluation of Glyphosate Application on Transgenic Soybean and its Relationship with Shikimic Acid. Planta Daninha, v.30, p.659-666, 2012.

GRYNSPAN, F.; CHERYAN, M. Phytate-calcium interactions with soybean protein. Journal of the American Oil Chemist’s Society, v.66, p.93-97, 1989.

JEZOVSEK, G.K. Uma nova proposta para o controle das ervas daninhas: o uso de plantas transgênicas. 1997. In: SIMPÓSIO SOBRE HERBICIDAS E PLANTAS DANINHAS, 1 Simpósio sobre herbicidas e plantas daninhas. Dourados: EMBRAPA-CPAO, 62-74.

LATTA, M.; ESKIN, M.A. Simple and rapid colorimetric method for phytate determination. Journal Agriculture Food Chemistry, v.28, p.1313-1315, 1980.

MARUR, C. J.; SODEK, L.; MAGALHÃES, A.C.N. Free amino acids in leaves of cotton plants under water deficit. Revista Brasileira Fisiologia Vegetal, v.6, p.103-108, 1994.

MATAVELI, L.R.V.; POHL, P.; MOUNICOU, S.; ARRUDA, M.A.Z.; SZPUNAR, J. A. Comparative study of element concentrations and binding in transgenic and non-transgenic soybean seeds. Metallomics, v.2, p. 781–832, 2010.

YEMM, E.W; COCKING, E.C. The determination of amino-acids with ninhydrin. Analyst, v.80, p.209-214, 1995.




DOI: https://doi.org/10.34117/bjdv7n6-283

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