Use of platelet-rich plasma in the treatment of ulcerative keratitis in equine – Case report/ Uso de plasma rico em plaquetas no tratamento de ceratite ulcerosa em eqüinos - Relato de caso

Maurício Orlando Wilmsen, Ana Carla da Silva Rizzi, Ana Carolina da Silva Rizzi, Caian Silva Correa, Karla Daniele Arboleya, Suélen Dalegrave


The platelet-rich plasma (PRP) has been applied to repair and regenerate different types of tissues. Currently, the use of platelet-rich plasma has demonstrated more and more effectiveness in the recovery of the lesion and in the gain of time in the treatment of the corneal keratitis or corneal ulcer in different species. The prevalence of traumas that affect the cornea in horses is high and represents the majority of ophthalmic injuries in this species. Due to its localization, it is more frequently subject to traumatic processes, such as corneal ulcer. The time it takes to make the diagnosis and start treatment may be decisive for the animal's prognosis. The aim of this study was to report a clinical case of ulcerative keratitis in a horse treated with platelet-rich plasma (PRP) associated with eye drops. A seven-year-old female horse was seen at an equestrian center, with a history of facial lesion evolving for six days, which was diagnosed as ulcerative keratitis. In the clinical exam the animal presented pain and intense agitation during the clinical evaluation besides corneal lesion, photophobia, blepharospasm, epiphora, conjunctival hyperemia, corneal edema, miosis and lacrimation. The animal was treated with autologous PRP and ophthalmic eye drops both with three TID drops. The PRP was obtained by collecting whole blood (80mL) by venipuncture of the right jugular vein in tubes containing sodium citrate, centrifuged and kept under refrigeration. During the treatment, antalgic therapy was performed using non-steroidal anti-inflammatory and the animal was kept with a tampon during the 15 days of treatment until the evolution of the case and the beginning of healing of the lesion site, demonstrating the efficacy of PRP treatment.


Autologus PRP, Cytokines, Growth factors, Epiphora, Oxidative stress.

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ABDEL-SAEED, H., SALEM, N.Y. Evaluation of total antioxidant capacity, malondialdehyde, catalase, proteins, zinc, copper and IgE response in ovine verminous pneumonia. Internacional Journal of Veterinary Science,v.8, n.4, p. 255–258. 2019.

ACOSTA, L., CASTRO, M., FERNANDEZ, M., OLIVIERES, M., GOMEZ-DEMEL, E., TARTARA, L. Treatment of corneal ulcers with platelet rich plasma. Archivos de la Sociedad Española de Oftalmologia, v.89, n.2, p. 48–52. 2014. doi: 10.1016/j.oftal.2013.09.002.

ALIZADEH, S., BALAGHOLI, S., BARADARAN-RAFII, A., DELFAZA, S., SAFI, S. Autologous platelet-rich plasma eye drops accelerate re-epithelialization of post-keratoplasty persistent corneal epithelial defects. Journal of Ophthalmic & Vision Research, v. 14, n.1, p.131-135. 2019. doi: 10.4103/jovr.jovr_279_17.

EVERTS, P.A., HOOGBERGEN, M.M., WEBER, T.A., DEVILEE, R.J., VAN MONFTORT, G., DE HING, I.H. Is the use of autologous platelet-rich plasma gels in gynecologic, cardiac, and general, reconstructive surgery beneficial? Current Pharmacethical Biotechnology, v.13, n.7, p.1163-1172. 2012. doi:10.2174/138920112800624346.

FARGHALI, H.A., ABDELKADER, N.A., ABUBAKR, H.O., RAMADAN, E.S., KHATTAB,M.S., SALEM, N.H.Y., EMAM, I.A. Corneal Ulcer in Dogs and Cats: Novel Clinical Application of Regenerative Therapy Using Subconjunctival Injection of Autologous Platelet-Rich Plasma. Frontiers Veterinary Sciences, v.8. 2021. doi: 10.3389/fvets.2021.641265.

FINI, M.E., GIRARD, M.T., MATSUBARA, M., BARTLETT, J.D. Unique regulation of the matrix metalloproteinase, gelatinase b. Investigative Ophthalmology & Visual Science, v.36, n.3, p. 622–633. 1995.

LI, D.Q., SHANG, T.Y., KIM, H.S., SOLOMON, A., LOKESHWAR, B.L., PFLUGFELDER S.C. Regulated expression of collagenases MMP-1,−8, and−13 and stromelysins MMP-3,−10, and−11 by human corneal epithelial cells. Investigative Ophthalmology & Visual Science, v.44, n. 6, p. 2928–2936. 2003. doi:10.1167/iovs.02-0874.

MARTINS, T., BARROS, C. Fifty years in the blink of an eye: a retrospective study of ocular and periocular lesions in domestic animals. Pesquisa Veterinária Brasileira, v.34, n.12, p.1215-1222, 2014.

MELLER, D., LI, D.Q., TSENG, S.C. Regulation of collagenase, stromelysin, and gelatinase b in human conjunctival and conjunctivochalasis fibroblasts by interleukin-1beta and tumor necrosis factor-alpha. Investigative Ophthalmology & Vision Science, v.41, n.10, p. 2922–2929. 2000.

SANCHEZ-GONZALEZ, D.J., MENDEZ, B.E., TREJO, B.N.I Platelet-rich plasma peptides: key for regeneration. Internal Journal of Peptides. 2012. doi:10.1155/2012/532519

TAMILMAHAN, P.; ZAMA, M.; PATHAK, R.; MUNEESWARAN, N.; KARTHIK, K. A retrospective study of ocular occurrence in domestic animals: 799 cases. Veterinary World, v.6, n.5, p.274-276, 2013. doi:10.5455/vetworld.2013.274-276.

WOUK, A.F., ULIAN, I., MULLER, G., SOUZA, A.L.G. Action of chondroitin sulfate sssociate to the ciprofloxacin corneal ulcers in horses. Revista Acadêmica Ciência Animal, v.4, p.11-20, 2006. doi:10.7213/cienciaanimal.v4i4.9465.



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