Predictive biomarkers of bronchopneumonia in bovine neonates / Biomarcadores preditivos de broncopneumonia em neonatos bovinos

Ismael Mateus Cavazini, Joao Alveiro Alvarado-Rincón, Josiane Oliveira Feijó, Carolina Bespalhok Jacometo, Antônio Amaral Barbosa, Francisco Augusto Burkert Del Pino, Marcio Nunes Corrêa, Viviane Rohrig Rabassa


The aim of this study was to determine predictive markers of bronchopneumonia in bovine neonates. A total of 70 Holstein calves were monitored from birth to the sixth week of life. Eighteen of the calves were selected and divided into two groups: bronchopneumonia group (n=12) and healthy group (n=6). For gene expression analysis, on the first day of life all animals were submitted to transcutaneous hepatic biopsy. A clinical examination was performed twice a week, where respiratory rate and nasal secretion were monitored to establish the diagnosis of bronchopneumonia. In addition, serum total plasma protein (TPP), gamma glutamyl transferase (GGT), albumin, paraoxonase-1 (PON1) and haptoglobin levels were determined on days -28, -21, -14 and -7, in relation to the day of diagnosis of bronchopneumonia. PON1 (P<0.0001) and TPP (P=0.01) were lower in animals that presented bronchopneumonia, while GGT tended to be lower in the healthy group (P=0.07). Furthermore, there were no differences in the expression of genes linked to the metabolism and inflammatory profile of calves at birth, between healthy animals and those that later presented bronchopneumonia. Therefore, this study suggests that PON1 and TPP are important early markers in the diagnosis of bronchopneumonia in calves.


biomarkers, bronchopneumonia, paraoxonase, predictors, total plasma protein


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