Multiplex PCR for molecular characterization of isolated strains of granuloma in sheep from state of Sergipe and Antimicrobial Susceptibility Test (AST) / PCR Multiplex para caracterizaçao molecular de linhagem isolada de granuloma em ovelha no estado de Sergipe e Teste de suceptibilidade antimicrobiana

Lumar Lucena Alves, Daniela Droppa Almeida, Caroline de Santana Ferreira, Ioná Brito, Glenda Amaral da Silva, Lívia Maria do Amorim Costa Gaspar, Afonso Celso Dórea, Francine Ferreira Padilha

Abstract


Corynebacterium pseudotuberculosis is the etiologic agent responsible for Caseous Lymphadenitis (CLA), an infectious disease that affects cattle and horses, but mostly small ruminants such as sheep and goats. This pathology, also known as the "bad seed," causes abscesses formation in the contaminated animal tissue, which can be superficial or visceral. As it is present in several Brazilian states, it is necessary to find out effective ways of diagnostic, prophylactic and effective treatment for CLA in attempt to eliminate this pathogen in rural areas. In Sergipe, the goat farming has progressively gained recognition in recent years and has surpassed over 50% of sheep flocks in the last decade. Therefore, Sergipe has presented a considerable development in sheep farming as well as it has expanded its potential commercialization regarding these animals and their derivatives. However, with the lack of epidemiological analysis, it becomes difficult to know both the presence and prevalence of the disease in the state. Thus, this study aimed to identify the microorganism isolated from a sheep granuloma in Sergipe not only by biochemical tests, considered the gold standard for C. pseudotuberculosis but also by molecular analysis through multiplex PCR, as well as to evaluate the antimicrobial susceptibility test.


Keywords


Corynebacterium pseudotuberculosis, Caseous Lymphadenitis, Granuloma

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DOI: https://doi.org/10.34117/bjdv.v7i5.29869

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