Supplementation with detox juice added with probiotic improves metabolic and inflammatory parameters in healthy individuals / Detox juice added with probiotic improves metabolic and inflammatory parameters

Eduardo Ottobeli Chielle, Daniel Lamour, Eliandra Mirei Rossi, Bruno de Lai, Ana Paula Ottobelli Chielle

Abstract


Phytochemicals present in detox juices and probiotics have demonstrated protective effects on cardiovascular risk factors. The consumption of these products alone modulate inflammatory mechanisms and metabolic biomarkers. However, the effect of the combination of detox juice and probiotics has not yet been evaluated on metabolic and inflammatory parameters. Objective of the study was to verify the effects of supplementation with a detox juice added with probiotic on metabolic and inflammatory parameters in healthy individuals. A controlled cross-sectional study was carried out with 40 healthy volunteers (20 men and 20 women), aged between 18 and 50 years. The volunteers ingested 200mL of a detox juice plus probiotic for 30 days. Before and after supplementation, the anthropometric profile, metabolic parameters (Colesteol, triglycerides, LDL, HDL, glucose, HbA1c), concentrations of unltra sensitive reactive Protein C, Internelucin-6, activity Adenosine deaminase and DPP-IV. There was a slight improvement in anthropometric parameters and total cholesterol, a reduction in LDL-c concentrations (p 0.05), triglycerides (p=0.05) and a significant increase in HDL-c (p=0.002). There was a significant decrease in the activity of ADA and DPP-IV (p=0.01), together with a significant reduction in the concentration of IL-6 and CRP-us (p=0.01). The findings highlight that supplementation with probiotic detox juice improved the lipid profile and reduces inflammatory and metabolic markers, suggesting a possible positive effect in reducing the risk of cardiovascular disease in healthy volunteers, as it plays an important role as an anti-inflammatory agent and lipid-lowering. However, more robust studies with a prolonged treatment period should be conducted.


Keywords


Probiotics, Detox juice, Inflammation, Cardiovascular diseases

References


Avellone GVDG, Campisi D, Simone RD, Raneli G, Scaglione R, Licata G. (2006). Effects of moderate Sicilian red wine consumption on inflammatory biomarkers of atherosclerosis. European Journal of Clinical Nutrition 60, 41–47.

Bastard JP, Maachi M, Lagathu C, Kim MJ, Caron M, Vidal H, Capeau J, Feve B. (2006). Recent advances in the relationship between obesity, inflammation and insulin resistance. Eur Cytokine Netw. 17(1):4-12.

Bellé LP, Bitencourt PE, De Bona KS, Moresco RN, Moretto MB. (2012). Association between HbA1c and dipeptidyl peptidase IV activity in type 2 diabetes mellitus. Letter to the Editor. ClinicaChimicaActa. 413 1020–1021.

Bellé MH, Sari1 GT, Schetinger RC, Morsch VM, Boligon A, Athayde ML, Pigatto AS, Moretto MB. (2010). Syzygium cumini Extract Decrease Adenosine Deaminase, 5’Nucleotidase Activities and Oxidative Damage in Platelets of Diabetic Patients Karine S. De Bona1 , Luziane P. Cell Physiol Biochem. 26:729-738

Bengmark S. (2005). Synbiotics and the mucosal barrier in critically ill patients. Curr Opin Gastroenterol, 21(6): 712-716.

Bonomini F, Rodella LF, Rezzani R. (2015). Metabolic syndrome, aging and involvement of oxidative stress. Aging Dis. 6(2):109-20.

Chatterjee M. et al. (2011). Role of 5-lipoxygenase in resveratrol mediated suppression of 7,12-dimethylbenz(á) anthracene-induced mammary carcinogenesis in rats. European Journal of Pharmacology, v. 668, p.99–106.

Chielle EO, De Souza WM, Da Silva TP, Moresco RN, Moretto MB. (2016) Clin Biochem. .Adipocytokines, inflammatory and oxidative stress markers of clinical relevance altered in young overweight/obese subjects. May;49(7-8):548-53.

Chielle, E.O., Bonfanti, G., De Bona, K.S., Moresco, R.N., Moretto, M.B. (2015). Adenosine deaminase, dipeptidyl peptidase-IV activities and lipid peroxidation are increased in the saliva of obese young adult. ClinChem Lab Med. Jun;53(7):1041-7.

Chuang, C..; Mcintosh, M.K. (2011). Potential Mechanisms by Which Polyphenol-Rich Grapes Prevent Obesity-Mediated Inflammation and Metabolic Diseases. The Annual Review of Nutrition, v.31, p.155–76.

Cianciulli, A. et al. (2012). Modulation of NF-jB activation by resveratrol in LPS treated human intestinal cells results in downregulation of PGE2 production and COX-2 expression. Toxicology in Vitro, v. 26, p.1122–1128.

Csiszar, A. et al. (2006). Resveratrol attenuates TNF-á induced activation of coronary arterial endothelial cells: role of NF-kB inhibition. Am. J. Physiol. Heart. Circ. Physiol., v.291, p.1694-1699.

Crovesy, L., Ostrowski, M., Ferreira, D.M.T.P., Rosado, E.L., Soares-Mota, M. (2017). Effect of Lactobacillus on body weight and body fat in overweight subjects: a systematic review of randomized controlled clinical trials. Int J Obes (Lond). 41: 1607–1614.

Devaraj, S.; Xu, D.Y.; Jialal, I. (2003). C-Reactive Protein Increases Plasminogen Activator Inhibitor-1 Expression and Activity in Human Aortic Endothelial Cells: Implications for the Metabolic Syndrome and Atherothrombosis. Circulation, v.107, p.398-404.

El-Ghazely, M.H., Mahmoud, W.H., Atia, M.A., Eldip, E.M. (2016). Effect of probiotic administration in the therapy of pediatric thermal burn. Ann Burns Fire Disasters, 29(4): 268-272.

Estruch, R., Sacanella, E., Badia, E., Antunez, E., Nicolas, J.M., Fernandez-Sola, J. et al. (2004). Different effects of red wine and gin consumption on inflammatory biomarkers of atherosclerosis: a prospective randomized crossover trial. Effects of wine on inflammatory markers. Atherosclerosis 175 (1), 117– 123.

Fontana, L., Eagon, J.C., Trujillo, M.E., Scherer, P.E., Klein, S., et al. (2007). Visceral fat adipokine secretion is associated with systemic inflammation in obese humans. Diabetes. 56, 1010–1013.

Friedewald, W.T.; Levy, R.I.; Fredrickson, D.S. (1972). Estimation of concentration of low-density lipoprotein in plasma, without use of preparative ultracentrifuge.ClinicalChe mistr y, v. 18, p. 499-502.

Galdeano, C.M., De Moreno, L.A., Vinderola, G., Bonet, M.E.B. et al.(2007). Proposed model: mechanisms of immunomodulation induced by probiotic bacteria. Clin Vaccine Immunol, 14(5): 485-492.

Gessner, D. K. et al. (2012). Inhibition of the pro-inûammatory NF-kB pathway by a grape seed and grape marc meal extract in intestinal epithelial cells. Journal of Animal Physiology and Animal Nutrition, v. 96, p.1074–1083.

Gomes, A.C., De Sousa, R.G., Botelho, P.B., Gomes, T.L., Prada, P.O., and Mota, J.F. (2017). The additional effects of a probiotic mix on abdominal adiposity and antioxidant status: a double-blind, randomized trial. Obesity (Silver Spring). 25: 30–38.

Haddy, N., Sass, C., Droesch, S., Zaiou, M., et al. (2003). IL-6, TNF-alpha and atherosclerosis risk indicators in a healthy family population: the stanislas cohort. Atherosclerosis. 170(2):277-83.

Halliwell, B.; Whiteman, M. (2004) Measuring reactive species and oxidative damage in vivo and in cell culture: how should you do it and what do the results mean?. British journal of pharmacology, v. 142, n. 2, p. 231-255.

Hee Park, K. et al. (2013). Circulating irisin in relation to insulin resistance and the metabolic syndrome. The Journal of Clinical Endocrinology & Metabolism, v. 98, n. 12, p. 4899-4907.

Indrayanto. Y., Prasetyo, D.H. (2006). Probiotic effect of mortality, degree of intestinal inflammation, and IgA levels in sepsis mice model. MKB, 45(1): 10-15.

Indulekha, K., Surendar, J., Mohan, V. (2011). High sensitivity C-reactive protein, tumor necrosis factor-a, interleukin-6, and vascular cell adhesion molecule-1 levels in Asian Indians with metabolic syndrome and insulin resistance (CURES-105) J Diabetes Sci Technol. 5:982–8.

Jones, S.A., Horiuchi, S., Topley, N., Yamamoto, N., Fuller, G.M.. (2001). The soluble interleukin 6 receptor: mechanisms of production and implications in disease. FASEB J. Jan;15(1):43-58.

Katz, A., Nambi, S.S., Mather, K., Baron, A.D., Follmann, D.A., Sullivan, G., et al. (2000). Quantitative insulin sensitivity check inex: a simple, accurate method for assessing insulin sensitivity in humans. The Journal of Clinical Endocrinology and Metabolism. 85 (7): 2402-2410.

Kaur, G. et al. (2007). Effect of wine phenolics on cytokineinduced C-reactive protein expression. Journal of Thrombosis and Haemostasis, v.5, p.1309–1317.

Kim, J.; Cha, Y.N.; Surh, Y.J. (2010). A protective role of nuclear factor-erythroid 2-related factor-2 (Nrf2) in inûammatory disorders. Mutat. Res., v. 690, p.12-23.

Kubota, S. et al. (2010). Resveratrol Prevents Light-Induced Retinal Degeneration via Suppressing Activator Protein-1 Activation. The American Journal of Pathology, v.177, n.4.

Kumar, M. et al. (2012). Cholesterol-lowering probiotics as potential biotherapeutics for metabolic diseases. Experimental diabetes research, v. 2012.

Lançon, A. et al. (2012). Control of MicroRNA Expression as a New Way for Resveratrol to Deliver Its Beneûcial Effects. J. Agric. Food Chem., v. 60, p.878-8789.

Libby, P., Ridker, P.M., Maseri, A. (2002). Inflammation and atherosclerosis. Circulation. 105(9):1135-43.

Lohman, T.G.; Roche, A.F.; Martorel, R. (1988). Anthropometrics standartization reference manual. Ilinois: Human Kinetics.

Machado, P. G. et al. (2017). Elaboração de suco misto de frutas com potencial funcional e comparação com suco comercial “detox”. In: Revista do Congresso Sul Brasileiro de Engenharia de Alimentos. Vol. 9, n 8.

Nagatsu, T., Hino, M., Fuyamada, H., Hayakawa, T., Sakakibara, S. (1976). New chromogenic substrates for prolyldipeptidyl-aminopeptidase IV. Anal Biochem. 74:466-476.

Otarod, J. K.; Goldberg, I.J. (2004). Lipoprotein lipase and its role in regulation of plasma lipoproteins and cardiac risk. Current atherosclerosis reports, v. 6, n. 5, p. 335-342.

Overman, A. et al. (2010). Polyphenol-rich grape powder extract (GPE) attenuates inflammation in human macrophages and in human adipocytes exposed to macrophageconditioned media. International Journal of Obesity, v.34, p.800–808.

Papanicolaou, D.A. (2000). Interleukin-6: The Endocrine Cytokine. J Clin Endocrinol Metab. 85(3):1331-1333.

Rinne, M., Kalliomaki, M., Arvilommi, H., Salminen, S. et al. (2005). Effect of probiotics and breastfeeding on the bifidobacterium and lactobacillus/enterococcus microbiota and humoral immune responses. J Pediatr, 147(2): 186-191.

Roth, G.A., Forouzanfar, M.H., Moran, A.E., Barber, R., Nguyen, G., Feigin, V.L., Naghavi, M., Mensah, G.A., Murray, C.J. (2015). Demographic and epidemiologic drivers of global cardiovascular mortality. N Engl J Med. 372:1333–1341.

Senn, J.J., Klover, P.J., Nowak, I.A., Mooney, R.A. (2002), Interleukin-6 induces cellular insulin resistance in hepatocytes, Diabetes 51 3391–3399.

Silva, A.C.C. et al. (2016). Alimentos contendo ingredientes funcionais em sua formulação: revisão de artigos publicados em revistas brasileiras. Revista Conexão Ciência, v. 11, n. 2, p. 133-144.

Tunapong, W., Apaijal, N., Yasom, S., Tanajak, P., Wanchai, K., Chunchai, T. et al. (2018). Chronic treatment with prebiotics, probiotics and synbiotics attenuated cardiac function by improving cardiac mitochondrial dysfunction in male obese insulin-resistant rats. Eur J Clin Nutr. 57: 2091–2104.

Varavallo, M. A.; THOMÉ, J. N.; TESHIMA, E. (2008). Aplicação de bactérias probióticas para profilaxia e tratamento de doenças gastrointestinais. Semina: Ciências biológicas e da saúde, v. 29, n. 1, p. 83-104.

Vazquez-Agell, M., Sacanella, E., Tobias, E., Monagas, M., Antunez. E. et al. (2007). Inflammatory Markers of Atherosclerosis Are Decreased after Moderate Consumption of Cava (Sparkling Wine) in Men with Low Cardiovascular Risk. The Journal of Nutrition, Volume 137, Issue 10, October, Pages 2279–2284

Xia, Y., Khatchikian, G., Zweier, J.L. (1996). Adenosine deaminase inhibition prevents free radical-mediated injury in the postischemic heart. J Biol Chem. 71:10096-100102

Wright, B., Moraes, L.A., Kemp, C.F., Mullen, W., Crozier, A., Lovegrove, J.A., Gibbins, J.M. (2010). Structural basis for the inhibition of collagen-stimulated platelet function by quercetin and structurally related flavonoids. Br J Pharmacol. 159: 1312-1325.

Yang, R.L. et al. (2006). Effect of antioxidant capacity on blood lipid metabolism and lipoprotein lipase activity of rats fed a high-fat diet. Nutrition, v. 22, n. 11-12, p. 1185-1191.

Yu, D.M., Slaitini, L., Gysbers, V., Riekhoff, A.G., Kähne, T., Knott, H.M.,et al. (2011). Soluble CD26 / dipeptidyl peptidase IV enhances human lymphocyte proliferation in vitro independent of dipeptidyl peptidase enzyme activity and adenosine deaminase binding.Scand J Immunol. Feb;73(2):102-11.

YUN, J. et al. (2012). Resveratrol up-regulates SIRT1 and inhibits cellular oxidative stress in the diabetic milieu: mechanistic insights. Journal of Nutritional Biochemistry, v.23, p.699–705.




DOI: https://doi.org/10.34117/bjdv.v7i5.29769

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