Effect of moringa oleifera on the development of adult wistar rats/ Efeito da Moringa oleifera sobre o desenvolvimento de ratos wistar adultos

Marcela R. Rocha, Estelamar M. B. Teixeira, Geraldo Thedei Júnior, Rodolfo N. Almeida, Elisa N. F. Santos, Fernanda B. B. Jardim


INTRODUCTION: proteins of plant origin have gained prominence, interest and adepts in their consumption.

OBJECTIVE: to verify and compare the effects of the offer of Moringa oil, as the only protein source in the development of adult Wistar rats.

METHODS: the animals were divided into three groups that received standard AIN93M normoproteic, normoglycidic and normolipidic rations (12%, 83% and 5% respectively) and water ad libtum for three weeks, the protein sources being casein (control group - GC), Whey Protein (WP group) and Moringa oleifera flour (MO group). The flour was produced through leaves collected randomly in the city of Uberaba - MG from afforestation plants, which were sanitized with water and sanitized in 200-ppm hypochlorite solution for 15 minutes, in the Bromatology laboratory of the Federal Institute of Triângulo Mineiro (IFTM) - campus Uberaba - MG. They were dried and homogenized in an oven, with air circulation and renewal, for approximately 42 hours at 35 ° C. Then the material was crushed, sieved and the flour obtained was packed in airtight glass. The experimental design was in randomized blocks (RBD). ANOVA was used to compare the different groups with Tukey's post-test to compare the variables in relation to the groups.

RESULTS: there was no statistical difference between the different protein sources on the Lee Index, however the dietary intake of the MO group (613.6 ± 41.34) was higher (p <0.05) than the CG groups (528 ± 65, 62) and WP (488.8 ± 34.72) and lower body weight in grams (MO: 232.30 ± 17.00) than CG (276.10 ± 34.60) and WP (265.10 ± 17.80) (p <0.05). The animals were healthy, with no indication of malnutrition (loss of hair or weight) throughout the experiment and euthanasia.

CONCLUSIONS: Moringa oleifera leaf flour can be a good protein source for adult Wistar rats.


vegetable proteins, Moringa, leaf flour, Wistar rats, experimental nutrition, whey protein.

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Philippi, ST. Pirâmide dos alimentos: fundamentos básicos da nutrição. 2nd ed. São Paulo: Manole; 2015.

Mendes A, Gavioli L, Previdelli AN, Fisberg RM, Marchioni DML. The diet quality index evaluates the adequacy of energy provided by dietary macronutrients. Revista de Nutrição. 2015; 28 (4): 341-348.

Teixeira EMB, Carvalho MRB, Neves VA, Silva MA, Arantes-Pereira L. Chemical characteristics and fractionation of proteins from Moringa oleifera Lam. leaves. Food Chemistry. 15 de março de 2014; 147: 51-54.

Kayode RMO, Afolayan AJ. Cytotoxicity and effect of extraction methods on the chemical composition of essential oils of Moringa oleifera seeds Journal of Zhejiang University-SCIENCE B. 2015; 16 (8): 680-689.

Leone A, Spada A, Battezzati A, Schiraldi A, Aristil J, Bertoli S. Cultivation, genetic, ethnopharmacology, phytochemistry and pharmacology of Moringa oleifera leaves: An overview. International Journal of Molecular Sciences. 2015; 16 (6): 12791-12835.

Xu YB, Chen GL, Guo MQ. Antioxidant and anti-inflammatory activities of the crude extracts of Moringa oleifera from Kenya and their correlations with flavonoids. Antioxidants. 2019; 8 (8): 296.

Kim YJ, Kim HS. Screening Moringa species focused on development of locally available sustainable nutritional supplements. Nutrition Research and Practice. 2019; 13 (6): 529-534.

Ali MW, Ilays MZ, Saeed MT, Shin DH. Comparative assessment regarding antioxidative and nutrition potential of Moringa oleifera leaves by bacterial fermentation. Journal of Food Science and Technology. 2020; 57 (3): 1110-1118.

Porteous IR. Geometric differentiation. Cambridge: University Press, 1994.

Reeves PG, Nielsen FH, Fahey GC. AIN-93 Purified Diets for Laboratory Rodents: Final report of the American Institute of Nutrition Ad Hoc Writing Committee on the Reformulation of the AIN-76A Rodent Diet. The Journal of Nutrition. 1993; 123 (11): 1939–1951.

Miranda TM, Viana EDSM. Avaliação da qualidade proteica da ervilha. Global Science and Technology. 2017; 10 (1): 69-77. Português.

Silva MDLC, Speridião PDGL, Marciano R, Amâncio OMS, Morais TBD, Morais MBD. Effects of soy beverage and soy-based formula on growth, weight, and fecal moisture: experimental study in rats. Jornal de Pediatria. 2015; 91 (3): 306-312.

Ramos MDLM, Rodrigues GDCG, Soares WRG, Hiane PA, Ramos MIL, Almeida JAD, Sanches FLFZ. Suplementação com alteração de composição corporal de ratos submetidos ao exercício. Revista Brasileira de Medicina do Esporte. 2017; 23 (4): 294-299. Português.

Franzen JM, Zancanaro V, Ruppel Rocha RE, Mariano de Bitencuort R. Dieta com whey protein promove controle do peso corporal e não altera função renal em ratos Wistar sedentários. Anais eletrônicos Do International Symposium on Science and Biotechnology. 2015; 1 (1), 59-60. Retração em: https://unoesc.emnuvens.com.br/ISSB/article/view/7644. Português.

Freudenberg A, Petzke KJ, Klaus S. Dietary L-leucine and L-alanine supplementation have similar acute effects in the prevention of high-fat diet-induced obesity. Amino Acids. 2013; 44 (2): 519-528.

Tong X, Li W, Xu JY, Han S, Qin LQ. Effects of whey protein and leucine supplementation on insulin resistance in non-obese insulin-resistant model rats. Nutrition. 2014; 30 (9): 1076-1080.

Richter CK, Skulas-Ray AC, Champagne CM, Kris-Etherton PM. Plant protein and animal proteins: do they differentially affect cardiovascular disease risk? Advances in Nutrition. 2015; 6 (6): 712-728.

Gazzani D, Zamboni F, Spelta F, Ferrari P, Mattioli V, Cazzoletti L, et al. Vegetable but not animal protein intake is associated to a better physical performance: a study on a general population sample of adults. Food & Nutrition Research. 19 de setembro de 2019; 63. doi: 10.29219 / fnr.v63.3422. PMID: 31565042; PMCID: PMC6756227.

Comerford KB, Pasin G. Emerging evidence for the importance of dietary protein source on glucoregulatory markers and type 2 diabetes: different effects of dairy, meat, fish, egg, and plant protein foods. Nutrients. 2016; 8 (8): 446.

Devi S, Varkey A, Sheshshayee MS, Preston T, Kurpad AV. Measurement of protein digestibility in humans by a dual-tracer method. The American Journal of Clinical Nutrition. 2018; 107 (6): 984-991.

CONSULTATION, FAO Expert. Dietary protein quality evaluation in human nutrition. FAO Food and Nutrition Paper. 2011; 92: 1-66.

Adam CL, Gratz SW, Peinado DI, Thomson LM, Garden KE, Williams PA, Ross AW. Effects of dietary fibre (pectin) and/or increased protein (casein or pea) on satiety, body weight, adiposity and caecal fermentation in high fat diet-induced obese rats. PloS one. 2016; 11 (5): e0155871.

Faipoux R, Tomé D, Gougis S, Darcel N, Fromentin G. Proteins activate satiety-related neuronal pathways in the brainstem and hypothalamus of rats. The Journal of Nutrition. 2008; 138 (6): 1172-1178.

Fromentin G, Darcel N, Chaumontet C, Marsset-Baglieri A, Nadkarni N, Tomé D. Peripheral and central mechanisms involved in the control of food intake by dietary amino acids and proteins. Nutrition Research Reviews. 2012; 25 (1): 29-39.

Stengel A, Goebel-Stengel M, Wang L, Hu E, Karasawa H, Pisegna JR, Taché Y. High-protein diet selectively reduces fat mass and improves glucose tolerance in Western-type diet-induced obese rats. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology. 2013; 305 (6): R582-R591.

Cabeço LC, Akiba M, Calsa MS, Sartori DRDS, Vicentini-Paulino MDLM, Pinheiro DF. Dieta hiperlipídica com farinha de soja como fonte proteica: utilização na seleção de ratos propensos e resistentes à obesidade. Revista de Nutrição. 2010; 23 (3): 417-424. Português.

Johnstone AM. Safety and efficacy of high-protein diets for weight loss. Proceedings of the Nutrition Society. 2012; 71 (2): 339-349.

Batterham RL, Heffron H, Kapoor S, Chivers JE, Chandarana K, Herzog H, Withers DJ. Critical role for peptide YY in protein-mediated satiation and body-weight regulation. Cell Metabolism. 2006; 4 (3): 223-233.

Jakubowicz D, Froy O. Biochemical and metabolic mechanisms by which dietary whey protein may combat obesity and Type 2 diabetes. The Journal of Nutritional Biochemistry. 2013; 24 (1): 1-5.

Carrilho LH. Benefícios da utilização da proteína do soro de leite Whey Protein. RBNE-Revista Brasileira de Nutrição Esportiva. 2013; 7 (40): 195-203. Português.

Tranberg B, Madsen AN, Hansen AK, Hellgren LI. Whey-reduced weight gain is associated with a temporary growth reduction in young mice fed a high-fat diet. The Journal of Nutritional Biochemistry. 2015; 26 (1): 9-15.

Melesse A, Tiruneh W, Negesse T. Effects of feeding Moringa stenopetala leaf meal on nutrient intake and growth performance of Rhode Island Red chicks under tropical climate. Tropical and Subtropical Agroecosystems. 2011; 14 (2): 485-492.

Damor SV, Pawar MM, Ankuya KJ, Gami YM, Srivastava AK, Chauhan HD, Chaudhary KR. Effect of feeding different levels of Moringa (Moringa oleifera) leaves on growth performance of Mehsana goat kids. Significance. 2017; 1 (T2): T3.

Meel P, Gurjar ML, Nagda RK, Sharma MC, Gautam L. Growth performance of sirohi goat kids fed different levels of Moringa oleifera leaves Journal of Entomology and Zoology Studies. 2018; 6 (4): 786-791, 2018.

Von Diemen V, Trindade EN, Trindade MRM. Experimental model to induce obesity in rats. Acta Cirurgica Brasileira. 2006; 21 (6): 425-429.

DOI: https://doi.org/10.34117/bjdv.v7i5.29733


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