Combined morphological-compositional analysis of the interaction of collagen and chitosan based-materials with demineralized bovine dentin / Análise morfológica-composicional combinada da interação de materiais à base de colágeno e quitosana com dentina bovina desmineralizada

Sidnei Nahórny, Ivone Regina de Oliveira, Luís Eduardo Silva Soares


Treatments to promote the biomineralization of dentin are the focus of much research with different materials and processes. Biomineralization is a process mediated by an organic matrix in which organic macromolecules act as models for the nucleation and growth of mineral crystals to form hierarchically ordered hybrid materials, such as bones or teeth. This study describes the application of novel materials based on collagen and chitosan aimed to protect the dentin from erosive conditions. The dentin morphology of the bovine teeth treated with these materials was visualized using scanning electron microscopy (SEM). In additions, it was possible to confirm the data by analyzing the distribution of inorganic content of dentin by micro energy-dispersive X-ray fluorescence spectrometry (µ-EDXRF). The association of analytical techniques demonstrated greater surface coverage for the chitosan-fluoride followed by the hydrolyzed collagen. Both materials are promising for the application of dentin coverage in dentistry.


Chitosan, collagen, fluoride, scanning electron microscopy, micro energy-dispersive X-ray fluorescence spectrometry.

Full Text:



CAO, C.Y.; MEI, M.L.; LI, Q-L.; LO, E.C.M.; CHU, C.H. Methods for biomimetic remineralization of human dentine: a systematic review. International Journal of Molecular Sciences, v. 16, n. 3, p. 4615-4627, 2015.

DI FOGGIA, M.; PRATI, C.; GANDOLFI, M.G.; TADDEI, P. An in vitro study on dentin demineralization and remineralization: Collagen rearrangements and influence on the enucleated phase. Journal of Inorganic Biochemistry, v. 193, p. 84-93, 2019.

DOS SANTOS FILHO, J.M. et al. Ti: Sapphire femtosecond laser action on the intraradicular dentine: analysis in electronic scan microscopy. Brazilian Journal of Development, v. 7, n. 1, p. 8870-8880, 2021. DOI:10.34117/bjdv7n1-601

GAJJERAMAN, S.; NARAYANAN, K.; HAO, J.; QIN, C.; GEORGE, A. Matrix macromolecules in hard tissues control the nucleation and hierarchical assembly of hydroxyapatite. Journal of Biological Chemistry, v. 282, n. 2, p. 1193-1204, 2007.

KEEGAN, G.M.; SMART, J.D.; INGRAM, M.J.; BARNES, L-M.; BURNETT, G.R.; REES, G.D. Chitosan microparticles for the controlled delivery of fluoride. Journal of Dentistry v. 40, n. 3, p. 229-240, 2012.

KIM, Y.K.; GU, L-S.; BRYAN, T.E.; KIM, J.R.; CHEN, L.; LIU, Y.; YOON, J.C.; BRESCHI, L.; PASHLEY, D.H.; TAY, F.R. Mineralisation of reconstituted collagen using polyvinylphosphonic acid/polyacrylic acid templating matrix protein analogues in the presence of calcium, phosphate and hydroxyl ions. Biomaterials, v. 31, n. 25, p. 6618-6627, 2010.

NAHORNY, S.; ZANIN, H.; CHRISTINO, V.A.; MARCIANO, F.R.; LOBO, A.O.; SOARES, L.E.S. Multi-walled carbon nanotubes/graphene oxide hybrid and nanohydroxyapatite composite: A novel coating to prevent dentin erosion. Materials Science and Engineering: C, v. 79, p. 199-208, 2017.

NIU, L-N.; ZHANG, W.; PASHLEY, D.H.; BRESCHI, L.; MAO, J.; CHEN, J-H.; TAY, F.R. Biomimetic remineralization of dentin. Dental Materials, v. 30, n. 1, p. 77-96, 2014.

SOARES, L.E.S.; MELO, T.M.T.; DE SÁ BRANDIM, A.; DE OLIVEIRA, I.R. Chemical and morphological evaluation of enamel and dentin near cavities restored with conventional and zirconia modified glass ionomer subjected to erosion‐abrasion. Microscopy Research and Technique, v. 87, n. 7, p. 1114-1126, 2019.

SOARES L.E.S.; NAHORNY, S.; DE FARIA BRAGA, V., MARCIANO, F.R.; BHATTACHARJEE, T.T.; LOBO, A.O. Raman spectroscopy-multivariate analysis related to morphological surface features on nanomaterials applied for dentin coverage. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, v. 228, p. 117818, 2020.

TAY, F.R.; PASHLEY, D.H. Guided tissue remineralisation of partially demineralised human dentine. Biomaterials, v. 29, n. 8, p. 1127-1137, 2008.

URURAHY, M.S.; CURYLOFO-ZOTTI, F.A.; GALO, R.; NOGUEIRA, L.F.B.; RAMOS, A.P.; CORONA, S.A.M. Wettability and surface morphology of eroded dentin treated with chitosan. Archives of Oral Biology, v. 75, p. 68-73, 2017.

XU, Z.; NEOH, K.G.; LIN, C.C.; KISHEN, A. Biomimetic deposition of calcium phosphate minerals on the surface of partially demineralized dentine modified with phosphorylated chitosan. Journal of Biomedical Materials Research Part B: Applied Biomaterials, v. 98, n. 1, p. 150-159, 2011.



  • There are currently no refbacks.