Synthesis and application of molecularly imprinted polymers for the extraction of caffeine from food and beverage samples / Síntese e aplicação de polímeros com impressão molecular para a extracção de cafeína de amostras de alimentos e bebidas

Fernanda N. Feiteira, Wanderson A. da Silva, Annibal D. Pereira Netto, Wagner F. Pacheco


In this present study molecularly imprinted polymers were synthesized and characterized in order to extract caffeine from different food and beverage samples. The characteristics of molecular recognition of these polymers were used to develop a MIP-SPE methodology, a pre treatment able to eliminate interferences. The accuracy of the proposed method reached 96,4 ± 4,7% and the intermediate precision 2.30%. MIP-SPE cartridges here produced can be reused for at least 3 cycles, being much more affordable than the traditional commercial SPE cartridges. Caffeine was analyzed in fifteen food and beverage samples by the here developed method. It is particularly interesting to emphasize that the analyses of soft drinks and coffee powder samples made it possible to attest that, for this sort of samples, the proposed method is able to completely eliminate all the interferences present in these matrices, being unnecessary to apply chromatographic separation previously to analyte quantification. This result assures the method's selectivity and turns its application to these samples much faster, environmentally friendly and economically affordable. There are other works in literature that propose the use of MIP-SPE to perform caffeine determination in some food and beverage samples, but none of them reaches good accuracy and precision. Also, none of them presented selectivity enough not to need the use of chromatographic separation. 




molecularly imprinted polymers, caffeine, MIP-SPE.


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