“Obtaining transconjugant Escherichia coli costs isolated from chicken litter and chicks” “Obtenção de cepas transconjugantes de Escherichia coli isoladas de cama de frango e pintainhos”

Bruna Carolina Gonçalves, Miriam Dibo, Luís Eduardo de Souza Gazal, Leonardo Pinto Medeiros, Maísa Fabiana Menck Costa, Renata Katsuko Takayama Kobayashi, Benito Guimarães Brito, Gerson Nakazato

Abstract


It is known that chicken litter is home to many pathogenic and antimicrobial resistant bacteria, and in many cases, it is reused without prior treatment in the creation of subsequent batches. Thus, the work aimed to verify whether Escherichia coli strains that produce Extended Spectrum Beta-lactamases (ESBL) isolated from chicken beds in the state of Paraná, were able to transfer their genes to E. coli isolates from the intestinal microbiota of one day old chicks. For this study, four strains of E. coli producing ESBL were used, presenting the blaCTX-M1 gene isolated from chicken litter, to be the plasmid donors, and three strains isolated from chicks newly arrived at the shed, which showed resistance to only gentamicin. For the conjugation assays, the donor and recipient strains were used in a proportion of 1: 2 respectively, and the transconjugants selected for resistance to gentamicin and cefotaxime in specific concentrations. Transconjugant colonies were selected and their phylogenetic groups identified by the PCR technique. Of the 12 conjugations performed, three presented transconjugants harboring the blaCTX-M1 gene, indicating that there is a possibility that this gene could be transferred to E. coli of the microbiota. Thus, in the environment of the farms, the transmission of plasmids of resistance from bacteria present in the chicken litter to bacteria from the microbiota of the newly arrived chicks may occur. The fact that chicken litter is reused without proper treatment contributes to the maintenance and dissemination of genetic determinants for ESBL in broiler chickens.


Keywords


Conjugation; Bacterial resistance; APEC.

References


ABPA. Associação Brasileira de Proteína Animal, Relatório Anual. 2020.

Bernal, M. P; Alburquerque, J. A.; Moral, R. Composting of animal manures and chemical criteria for compost maturity assessment. A review. Bioresource Technology, v. 100, n. 22, p. 5444-5453, 2009.

Clermont, O.; Bonacorsi, S.; Bingen, E. Rapid and simple Determination of the Escherichia coli phylogenetic group. Applied and Enviromental Microbiology, USA, ano 10, v. 66, p. 4555-4558, 7 jul. 2000.

Cyoia, P. S. et al. (2019). Distribution of ExPEC virulence factors, blaCTX-M, fosA3, and mcr-1 in Escherichia coli isolated from commercialized chicken carcasses. Frontiers in Microbiolology 9:3254. doi: 10.3389/fmicb.2018.03254

Dho-Moulin, M.; Fairbrother, J. M. Avian pathogenic Escherichia coli (APEC). Veterinary Research, v. 30, n. 2-3, p. 299-316, 1999.

Dos Santos, T. M. B.; De Lucas J.; Sakomura, N. K. Effects of broiler stocking density and poultry litter reuse in broiler performance and poultry litter production. Revista Portuguesa de Ciências Veterinárias, v. 553, p. 45–52, 2005.

Gazal, L. E. S. et al. Detection of ESBL/AmpC-Producing and Fosfomycin-Resistant Escherichia coli From Different Sources in Poultry Production in Southern Brazil. Frontiers in Microbioliology 11:604544. doi: 10.3389/fmicb.2020.604544

Gazal, L. E. S. et al. Presence of pathogenicity islands and virulence genes of extraintestinal pathogenic Escherichia coli (ExPEC) in isolates from avian organic fertilizer. Poultry Science, v. 94, n. 12, p. 3025-3033, 2015.

Kaper, J. B.; Nataro, J. P.; Mobley, H. L. T. Pathogenic Escherichia coli. Nature Reviews Microbiology, v. 2, n. 2, p. 123-140, 2004.

Puño-Sarmiento, J. et al. Identification of diarrheagenic Escherichia coli strains from avian organic fertilizers. International Journal of Environmental Research and Public Health, v. 11, n. 9, p. 8924-8939, 2014.

Virtuoso, M. C. S.; De Oliveira, D. G.; Dias, L. N. S. Reutilização da cama de frango. Revista Eletrônica Nutritime, [S. l.], ano 02, v. 12, n. 02, p. 3964-3979, 31 mar. 2015. Disponível em: www.nutritime.com.br. Acesso em: 24 ago. 2019.




DOI: https://doi.org/10.34117/bjdv7n2-474

Refbacks

  • There are currently no refbacks.