Production of carotenoids by Rhodotorula mucilaginosa using sugarcane juice (Saccharum officinarum) in the fermentation. / Produção de carotenóides pela Rhodotorula mucilaginosa utilizando sumo de cana (Saccharum officinarum) na fermentação.

Patricia Teixeira Damasceno Lobo, Hugo Neves Brandão, Sandra Aparecida Assis

Abstract


This study aims to investigate the production of carotenoids from pigmented yeasts isolated from Bahia’s semi-arid region, using sugarcane juice as an alternative fermentation medium.  The 23 full factorial statistical design was used to test the following independent variables: concentration of sugarcane juice (%) and yeast extract (g L-1). The Doehlert design was used to test the variables of initial pH and stirring speed. The maximum production obtained showed a concentration of 40% sugarcane juice, 6.0 g L-1 yeast extract, initial pH of 6.8 and stirring speed of 176 rpm, which enabled a carotenoid production of 1300 μg L-1. As for the cell growth, the maximum was obtained at the concentration of 43% sugarcane juice, 9.2 g L-1 yeast extract, with an initial pH of 6.8 and stirring speed of 176 rpm, resulting in production of 14.7 g L-1. The HPLC analysis showed the presence of β-carotene among the compounds produced by the yeast. The results showed the use sugarcane supplemented with yeast extract and by controlling environmental conditions such as initial pH and stirring speed, it is possible to promote an increase in the bioproduction of carotenoids and biomass of R. mucilaginosa.


Keywords


agro-industrial product, yeast, β- carotene

References


Fraser PD, Bramley PM. The biosynthesis and nutritional uses of carotenoids. Prog Lipid Res. 2004; 43:228–65.

Rashid M, Mazumdar RM. Anti-bacterial activity of pigments isolated from pigment-forming soil bacteria. Br J Pharm Res. 2014;4: 880–894.

Velmurugan P, Kamala-Kannan S, Balachandar V, Lakshmanaperumalsamy, P, Chae JC, Oh BT. Natural pigment extraction from five filamentous fungi for industrial applications and dyeing of leather. Carbohydr. Polym. 2010; 79: 262–268.

Hernández-Almanza A, Montañez-Sáenz JC, Martínez-Ávila C, Rodriguez-Herrera, R, Aguilar CN. Carotenoid production by Rhodotorula glutinis YB-252 in solid-state fermentation. Food Biosci. 2014; 7: 31–36.

Voiades C, Dima R. Effect of carbon source on carotenoid production by Rhodotorula sp. Arch Zooteh. 2011; 14:75-83

Yimyoo T, Yongmanitchai W, Alimtong S. Carotenoid production by Rhodosporidium paludigenum DMKU3-LPK4 using glycerol as the carbon source. Kasetsart. J. N. Sci. 2011; 45: 90-100

Luna WNS Acetilação do exopolissacarídeo (16)- β-D-glucana (lasiodiplodana): derivatização química e caracterização [dissertação]. Pato Branco (PR): Universidade Tecnológica Federal do Paraná; 2016

Reyes LH, Gomez JM, Kao KC. Improving carotenoids production in yeast via adaptive laboratory evolution Metabolic Engineering. 2014; 21: 26-33.

Kot AM, Błażejak S, Kieliszek M, Gientka I, Bryś J. Applied Biochemistry and Biotechnology Simultanous production of lipids and carotenoids by the red yeast Rhodotorula from waste glycerol fraction and potato wastewater. J. Applied Biochemistry and Biotechnology. 2019; 189:589–607

Otero DM, Bulsing BA, Huerta KM, Rosa CA, Zambiazi RC, Burkert CAV, Burkert JFM. Carotenoid-producing yeasts in the brazilian biodiversity: isolation, identification and cultivation in agroindustrial waste. Braz. J Chem Eng. 2019; 36: 117-129.

Sharma R, Ghoshal G. Optimization of Carotenoids production by Rhodotorula mucilaginosa (MTCC-1403) using agro-industrial waste in Bioreactor: A statistical approach. Appl Biotechnol. Rep. 2020; 25.

Bagy MMK., Abd-Alla, MH, Nafady, NA, Morsy, FM. Mahmoud, G.A.E Bioconversion of plant wastes to β-carotene by Rhodotorula glutinis KU550702. Eur J Biol Res. 2016; 6, 226–241.

Bhosale P, Gadre RV β-Carotene production in sugarcane molasses by a Rhodotorula glutinis mutant. J Ind Microbiol Biot. 2001; 26: 327–3.

Abdelhafez, AA, Husseiny SM, Ali AA, Sanad HM. Optimization of b-carotene production from agro-industrial by-products by Serratia marcescens ATCC 27117 using Plackett–Burman design and central composite design, Ann. Agric Sci. 2016; 61:87-96.

Sena AR, Koblitz MGB, Neto AG, Uetanabaro APT. Selection of fungi from the Bahian semi-arid that secrete hydrolases of interest in food. Sitientibus. 2006; 35:.91-98

Kurtzman CP, Fell JW. The yeasts a taxonomic study. Amsterdam: Elsevier, (1998).

Miller GL. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 1959;31: 426-28.

Sedmak JJ, Weerasinghe DK, Jolly SO Extraction and quantitation of astaxanthin from Phaffia Rhodozyma. Biotechnl Tech. 1990; 4:107-12.

Peterson WJ Quantitative determination of the carotenoids in yeasts of the genus Rhodotorula. J Bacteriol 1958; 75:586-91.

Buzzini P, Innocenti M, Turchetti B, Libkind D, Broock MV, Mulinacci N. Carotenoid profile of yeast belonging to the gênero Rhodotorula, Rhodosporidium, Sporobolomyces e Sporidiobolus. Can. J. Microbiol. 2007; 53:1024-31.

Statistica (Data Analysis Software System), v. 7, StatSoft, Inc, Tulsa, OK, USA; 2008. http://www.statsoft.com

Banzatto D., Freita LA, Carneiro MJMR. Production of carotenoids by Rhodotorula rubra grown in juice, molasses and sugarcane syrup. Ciênc. Tecnol. Aliment. 2013; 33:14-18.

Costa W A, Padilha EA, Oliveira Júnior SD, Silva FLH, Silva J, Ancântara MA, Ferrari M, Santos E S. Oil-lipids, carotenoids and fatty acids simultaneous production by Rhodotorula mucilaginosa CCT3892 using sugarcane molasses as carbon source. Brazilian J Food Technol. 2020; 23.

Valduga E, Tatsch PO, Tiggemann L, Treichel H, Toniazzo G, Zeni J, Di Luccio M. Carotenoid production: microorganisms as a source of natural pigments. Química Nova. 2009; 32: 2429-2436.

Santos N.P et al. Assimilation of amino acids present in must based on sugarcane juice by Saccharomyces cerevisiae under fermentative stress. Braz. J. of Develop., Curitiba, v. 6, n.6, p.33971-33983jun.2020.

Yadav KS, Prabha R. Production of Intracellular Carotenoid Pigment from Wild Strains of Rhodotorula. Int J Curr Microbiol Appl Sci. 2017; 5: 679-683

Liu YS, Wu JY, Ho K. Characterization of oxygen transfer conditions and their effects on Phaffia rhodozyma growth and carotenoid. Chem Eng J. 2006; 27:331-335.

Kanzy HM, Nasr NF, El-Shazly HAM, Barakat OS. Optimization of Carotenoids production by yeast strains of Rhodotorula using salted cheese whey. Int J Curr Microbiol App Sci. 2015; 4: 456-469

Squina, F. M.; Yamashita, F.; Pereira, J. L.; Mercadante, A. Z. Production of carotenoids by Rhodotorula rubra and R. glutinis in culture medium supplemented with sugar cane juice. Food Biotechnology. v. 16, p. 227-35, 2002. DOI:10.1081/FBT-120016776

Squina FM, Mercadante A Z. HPLC analysis of carotenoids from five Rhodotorula strains. Rev Bras Ciênc Farm. 2004; 39:309-18.

Cheng, Y.T., Yang, C.F. Using the strain Rhodotorula mucilaginosa to produce carotenoids from food waste. J. Taiwan Inst. Chem. E .2016; 61, 270–275.




DOI: https://doi.org/10.34117/bjdv7n2-455

Refbacks

  • There are currently no refbacks.