Efeito da irrigação subgengival com seringa e ultrassônica passiva nos níveis de IL-1β, IL-6 e TNF-α na periodontite experimental em ratos / Effect of subgingival syringe irrigation and passive ultrasonic irrigation on IL-1β, IL-6 and TNF-α levels in experimental periodontitis in rats

Monique Maria Melo Mouchrek, Cláudio Vanucci Silva de Freitas, Lucilene Amorim Silva, Flávia Raquel Fernandes do Nascimento, Hugo Leonardo Melo Dias, Erick Miranda Souza, Luc W M Van der Sluis, Vanessa Camila da Silva

Abstract


Introdução: A persistência de sítios com doença periodontal após raspagem e alisamento radicular desencadeou a realização de estudos buscando um tratamento complementar. Considerando a melhor eficácia da irrigação ultrassônica na endodontia, buscou-se adaptar essa técnica na periodontia. Dessa forma, o objetivo deste estudo foi avaliar se a irrigação subgengival com seringa (IS) e/ou a irrigação subgengival ultrassônica passiva (ISUP) com soro fisiológico (SF), hipoclorito de sódio (HS) e extrato de própolis (PRO) influencia na concentração de Il-1β, IL-6 e TNF-α em periodontite experimental induzida em ratos. Métodos: Periodontite Experimental foi induzida com ligadura nos primeiros molares inferiores de 35 ratos Wistar. Após 14 dias, as ligaduras foram removidas, e os animais submetidos à raspagem, seguido por IS no lado esquerdo e ISUP no direito, com SF 0,9%, HS 0,1% e PRO 11%. Os animais foram sacrificados 2 dias após o tratamento e submetidos a análise ELISA para avaliação dos níveis das citocinas IL-1β, IL-6 e TNF-α. Os dados foram submetidos à análise estatística com nível de significância (α) de 5%.

Resultados: A IL-1β aumentou de forma estatisticamente significante no grupo raspagem (p=0,015) e reduziu no grupo de IS com HS (p=0,016). A IL-6 reduziu nos grupos de IS com SF, HS e PRO em relação ao grupo raspagem (p=0,007) e aumentou de forma estatisticamente significante no grupo ISUP em relação à IS (p=0,033). O TNF-α e as soluções irrigantes não apresentaram diferença estatística entre os grupos. Conclusão: As técnicas de irrigação com seringa associadas à raspagem apresentaram uma redução da concentração das citocinas inflamatórias quando comparadas ao tratamento somente de raspagem.

 


Keywords


Irrigação terapêutica. Doenças Periodontais. Terapia por ultrassom. Modelos animais de doenças.

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DOI: https://doi.org/10.34117/bjdv7n2-259

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