Efeito da irrigação subgengival com seringa e ultrassônica passiva nos níveis de IL-1β, IL-6 e TNF-α na periodontite experimental em ratos / Effect of subgingival syringe irrigation and passive ultrasonic irrigation on IL-1β, IL-6 and TNF-α levels in experimental periodontitis in rats
Abstract
Introdução: A persistência de sítios com doença periodontal após raspagem e alisamento radicular desencadeou a realização de estudos buscando um tratamento complementar. Considerando a melhor eficácia da irrigação ultrassônica na endodontia, buscou-se adaptar essa técnica na periodontia. Dessa forma, o objetivo deste estudo foi avaliar se a irrigação subgengival com seringa (IS) e/ou a irrigação subgengival ultrassônica passiva (ISUP) com soro fisiológico (SF), hipoclorito de sódio (HS) e extrato de própolis (PRO) influencia na concentração de Il-1β, IL-6 e TNF-α em periodontite experimental induzida em ratos. Métodos: Periodontite Experimental foi induzida com ligadura nos primeiros molares inferiores de 35 ratos Wistar. Após 14 dias, as ligaduras foram removidas, e os animais submetidos à raspagem, seguido por IS no lado esquerdo e ISUP no direito, com SF 0,9%, HS 0,1% e PRO 11%. Os animais foram sacrificados 2 dias após o tratamento e submetidos a análise ELISA para avaliação dos níveis das citocinas IL-1β, IL-6 e TNF-α. Os dados foram submetidos à análise estatística com nível de significância (α) de 5%.
Resultados: A IL-1β aumentou de forma estatisticamente significante no grupo raspagem (p=0,015) e reduziu no grupo de IS com HS (p=0,016). A IL-6 reduziu nos grupos de IS com SF, HS e PRO em relação ao grupo raspagem (p=0,007) e aumentou de forma estatisticamente significante no grupo ISUP em relação à IS (p=0,033). O TNF-α e as soluções irrigantes não apresentaram diferença estatística entre os grupos. Conclusão: As técnicas de irrigação com seringa associadas à raspagem apresentaram uma redução da concentração das citocinas inflamatórias quando comparadas ao tratamento somente de raspagem.
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DOI: https://doi.org/10.34117/bjdv7n2-259
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