DNA Barcode confirms the reallocation of Alona kaingang Sousa, Elmoor-Loureiro & Santos, 2015 (Crustacea, Cladocera, Chydoridae) to Ovalona kaingang and gives clues to the puzzle involving the pulchella-group / DNA Barcode confirma a realocação de Alona kaingang Sousa, Elmoor-Loureiro & Santos, 2015 (Crustacea, Cladocera, Chydoridae) para Ovalona kaingang e dá indícios para o “quebra cabeça” envolvendo o grupo pulchella

Cínthia Bruno de Abreu, Maria José dos Santos-Wisniewski, Tereza Cristina Orlando


Embraced within the pulchella-group, which covers 14 species of the genus Alona, Ovalona kaingang was one of the Alona species recently translocated to Ovalona genus by Sinev 2015. Also, it can be confused with Coronatella rectangula, when external general characters were considered. In order to verify O. kaingang molecular identity, we sequenced the DNA barcode region (COI), an efficient tool for this type of analysis. Our results indicate genetic divergence ranging from 4.1% to 23.1% among O. kaingang with other Ovalona and Leberis species analyzed, and corroborate Alona species from the pulchella-group translocation to the new Ovalona genus placement. In addition, the DNA barcoding analysis can be applied not only in future taxonomic revisions as in the resolution of troubles for the puzzle involving the Aloninae species taxonomy.


COI, molecular taxonomy, Aloninae, zooplankton, genetic divergence.


Belyaeva, M. & Taylor, D.J. (2009) Cryptic species within the Chydorus sphaericus species complex (Crustacea: Cladocera) revealed by molecular markers and sexual stage morphology. Molecular Phylogenetics and Evolution, 50, 534-546.

Castilho, M.C.D., Wisniewski, M.J.D., de Abreu, C.B. & Orlando, T.C. (2015) Life history and DNA barcode of Oxyurella longicaudis (Birgei, 1910) (Cladocera, Anomopoda, Chydoridae). Zoological Studies, 54,1-7.

Costa, F.O., deWaard, J.R., Boutillier, J., Ratnasingham, S., Dooh, R.T., Hajibabaei, M. & Hebert, P.D.N. (2007) Biological identifications through DNA barcodes: the case of the Crustacea. Canadian Journal of Fisheries and Aquatic Sciences, 64, 272-295.

Elias-Gutierrez, M. & Valdez-Moreno, M. (2008) A now cryptic species of Leberis Smirnov, 1989 (Crustacea, Cladocera, Chydoridae) from the Mexican semi-desert region, highlighted by DNA barcoding. Hidrobiologica, 18, 63-74.

Elias-Gutierrez, M., Jeronimo, F.M., Ivanova, N.V., Valdez-Moreno, M. & Hebert, P.D.N. (2008) DNA barcodes for Cladocera and Copepoda from Mexico and Guatemala, highlights and new discoveries. Zootaxa, 1839, 1-42.

Hebert, P.D.N., Ratnasingham, S. & deWaard, J.R. (2003) Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species. Proceedings of the Royal Society B-Biological Sciences, 270, S96-S99.

Ivanova NV, Waard JR, Hebert PDN (2006) An inexpensive, automation friendly protocol for recovering high-quality DNA. Mol Ecol Notes 6 998–1002.

Jeffery, N.W., Elias-Gutierrez, M. & Adamowicz, S.J. (2011) Species Diversity and Phylogeographical Affinities of the Branchiopoda (Crustacea) of Churchill, Manitoba,

Canada. Plos One, 6(5), e18364.

Kotov, A.A., Korovchinsky, N.M., Sinev, A.Y. & Smirnov, N.N. (2011) Cladocera (Crustacea, Branchiopoda) from the Zeya River Basin (Amur Region, Russian Federation). 3. Systematic-Faunistical and Zoogeographical Analyses. Zoologichesky Zhurnal, 90, 402-411.

Prosser, S., Martinez-Arce, A. & Elias-Gutierrez, M. (2013) A new set of primers for COI amplification from freshwater microcrustaceans. Molecular Ecology Resources,

, 1151-1155.

Rocha, O., Santos-Wisniewski, M.J. & Matsumura-Tundisi, T. (2011) Checklist de Cladocera de água doce do Estado de São Paulo. Biota neotrop.(Online, Ed. port.), 11,


Sacherova, V. & Hebert, P.D.N. (2003) The evolutionary history of the Chydoridae (Crustacea : Cladocera). Biological Journal of the Linnean Society, 79, 629-643.

Silva, E.D., de Abreu, C.B., Orlando, T.C., Wisniewski, C. & dos Santos-Wisniewski, M.J. (2014) Alona iheringula Sinev & Kotov, 2004 (Crustacea, Anomopoda,

Chydoridae, Aloninae): Life Cycle and DNA Barcode with Implications for the Taxonomy of the Aloninae Subfamily. Plos One, 9, e97050.

Sinev, A.Y. & Elmoor-Loureiro, L.M.A. (2010) Three new species of chydorid cladocerans of subfamily Aloninae (Branchipoda: Anomopoda: Chydoridae) from

Brazil. Zootaxa, 2390, 1-25.

Sinev, A.Y. & Silva-Briano, M. (2012) Cladocerans of genus Alona Baird, 1843 (Cladocera: Anomopoda: Chydoridae) and related genera from Aguascalientes State,

Mexico. Zootaxa, 3569, 1-24.

Sinev, A.Y. (2015) Revision of the pulchella-group of Alona s. lato leads to its translocation to Ovalona Van Damme et Dumont, 2008 (Branchiopoda: Anomopoda:

Chydoridae). Zootaxa, 4044, 451-492.

Sousa, F.D.R., Elmoor-Loureiro, L.M.A. & Santos, S. (2015a) Alona kaingang (Crustacea, Cladocera, Aloninae): a new species of the pulchella-group, with identification key to Neotropical species. Zoological Studies, 54. 1-12.

Sousa, F.D.R., Elmoor-Loureiro, L.M.A. & Santos, S. (2015b) Redescription of Coronatella poppei (Richard, 1897) (Crustacea, Branchiopoda, Chydoridae) and a revision of the genus in Brazil, with descriptions of new taxa. Zootaxa, 3955, 211-244.

Tamura, K., Stecher, G., Peterson, D., Filipski, A., Kumar, S. (2013). MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular Biology Evolution, 30, 2725–2729.

Van Damme, K. & Dumont, H.J. (2008a) Further division of Alona Baird, 1843: separation and position of Coronatella Dybowski & Grochowski and Ovalona gen.n. (Crustacea: Cladocera). Zootaxa, 1960, 1-44.

Van Damme, K. & Dumont, H.J. (2008b) The 'true' genus Alona Baird, 1843 (Crustacea: Cladocera: Anomopoda): position of the A. quadrangularis-group and description of a new species from the Democratic Republic of Congo. Zootaxa, 1943, 1-25.

Van Damme, K., Bekker, E.I. & Kotov, A.A. (2013) Endemism in the Cladocera (Crustacea: Branchiopoda) of Southern Africa. Journal of Limnology, 72, 440-463.

Van Damme, K., Kotov, A.A. & Dumont, H.J. (2010) A checklist of names in Alona Baird 1843 (Crustacea: Cladocera: Chydoridae) and their current status: an analysis of the taxonomy of a lump genus introduction. Zootaxa, 2330, 1-63.

Van Damme, K., Sinev, A.Y. & Dumont, H.J. (2011) Separation of Anthalona gen. n. from Alona Baird, 1843 (Branchiopoda: Cladocera: Anomopoda): morphology and evolution of scraping stenothermic alonines. Zootaxa, 2875, 1-64.

Viti, T., Wisniewski, C., Orlando, T.C. & dos Santos-Wisniewski, M.J. (2013) Life history, biomass and production of Coronatella rectangula (Branchiopoda, Anomopoda, Chydoridae) from Minas Gerais. Iheringia Serie Zoologia, 103, 110-117

DOI: https://doi.org/10.34117/bjdv7n2-191


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