Junction communication in the immune system: modulation of the GAP junctions by infection with Toxoplasma gondii / Comunicação juncional no sistema imunológico: modulação das junções GAP em infecção por Toxoplasma gondii

Authors

  • Gabriella Oliveira Alves Moreira de Carvalho
  • Olga Maria de Jesus Souza
  • Daniel Rodrigues Souza Brazilian Journals Publicações de Periódicos, São José dos Pinhais, Paraná
  • Raquel do Nascimento de Souza
  • Thiago Manchester de Mello
  • Regina Coeli dos Santos Goldenberg
  • Sérgio Henrique Seabra
  • Fabio da Silva de Azevedo Fortes

DOI:

https://doi.org/10.34117/bjdv7n1-281

Keywords:

Gap junction, Macrophages, Toxoplasma gondii.

Abstract

RETOMAR

O Toxoplasma gondii é um protozoário parasita responsável pela toxoplasmose, podendo estar causando problemas em diferentes sistemas. Algumas dessas complicações estão associadas à alteração da comunicação intercelular mediada por Junctions Communicators que permite a comunicação direta entre os tecidos. No entanto, ainda existem sistemas que não são totalmente consistentes com a comunicação juncional, incluindo o sistema imunológico inato, representado pelos macrófagos. Assim, neutralizou a cultura da linha celular do macrófago J774-G8 infectada pela cepa RH de Toxoplasma em sua forma de taquizoíta.Os resultados revelaram que nas células J774-G8 as proteínas Cx43 e Phalloidina interagem na membrana plasmática da linhagem J774-G8, sofrendo uma sensibilidade redução na membrana após 72 horas da infecção pelo parasita. Toxoplasma gondii . A avaliação da expressão da proteína Cx43 por transferência imunoeletroforética alterada (elevada) em células de macrófagos J774-G8 infectadas com o parasita Toxoplasma gondii 24 e 48 horas em comparação com células não infectadas.

References

ALLISON, A. C.; DAVIES, Poo; DE PETRIS, S. Role of contractile microfilaments in macrophage movement and endocytosis. Nature New Biology, v. 232, n. 31, p. 153-155, 1971.

BENCHIMOL, M. et al. The cytoskeleton of resident peritoneal mouse macrophages. Microscopia electronica y biologia celular: organo oficial de las Sociedades Latinoamericana de Microscopia Electronica e Iberoamericana de Biologia Celular, v. 9, n. 2, p. 177-191, 1985.

CHANSON, Marc et al. Gap junctional communication in tissue inflammation and repair. Biochimica et Biophysica Acta (BBA)-Biomembranes, v. 1711, n. 2, p. 197-207, 2005.

CONTRERAS, Jorge E. et al. Role of connexin-based gap junction channels and hemichannels in ischemia-induced cell death in nervous tissue. Brain Research Reviews, v. 47, n. 1-3, p. 290-303, 2004.

COSTA, P. C. S. et al. Sera from chronic chagasic patients depress cardiac electrogenesis and conduction. Brazilian Journal of Medical and Biological Research, v. 33, n. 4, p. 439-446, 2000.

DE CARVALHO, Antonio C. Campos et al. Gap junction disappearance in astrocytes and leptomeningeal cells as a consequence of protozoan infection. Brain research, v. 790, n. 1-2, p. 304-314, 1998.

DHEIN, Stefan. Gap junction channels in the cardiovascular system: pharmacological and physiological modulation. Trends in pharmacological sciences, v. 19, n. 6, p. 229-241, 1998.

DOS SANTOS, Thiago Alves Teixeira et al. Phosphatidylserine exposure by Toxoplasma gondii is fundamental to balance the immune response granting survival of the parasite and of the host. PLoS One, v. 6, n. 11, p. e27867, 2011.

EUGENÍN, Eliseo A. et al. TNF-? plus IFN-? induce connexin43 expression and formation of gap junctions between human monocytes/macrophages that enhance physiological responses. The Journal of Immunology, v. 170, n. 3, p. 1320-1328, 2003.

FEITOSA, Thais Ferreira et al. Anti-Toxoplasma gondii antibodies in mammals, birds and reptiles at the zoological-botanical park in João Pessoa, Paraíba, Brazil. Arquivos do Instituto Biológico, v. 84, 2017.

FORTES, F.S.A et al. Modulation of intercellular communication in macrophages: possible interactions between GAP junctions and P2 receptors. Journal of Cell Science, v. 117, n. 20, p. 4717-4726, 2004.

FUJIMOTO, J. et al. Molecular cloning and characterization of focal adhesion kinase of Drosophila melanogaster. In: MOLECULAR BIOLOGY OF THE CELL. 8120 WOODMONT AVE, STE 750, BETHESDA, MD 20814-2755 USA: AMER SOC CELL BIOLOGY, 1997. p. 2324-2324.

GAIETTA, Guido et al. Multicolor and electron microscopic imaging of connexin trafficking. Science, v. 296, n. 5567, p. 503-507, 2002.

GIEPMANS, Ben NG. Gap junctions and connexin-interacting proteins. Cardiovascular research, v. 62, n. 2, p. 233-245, 2004.

HANSSON, Elisabeth; SKIÖLDEBRAND, Eva. Coupled cell networks are target cells of inflammation, which can spread between different body organs and develop into systemic chronic inflammation. Journal of Inflammation, v. 12, n. 1, p. 44, 2015.

HERVÉ, Jean-Claude; BOURMEYSTER, Nicolas; SARROUILHE, Denis. Diversity in protein–protein interactions of connexins: emerging roles. Biochimica et Biophysica Acta (BBA)-Biomembranes, v. 1662, n. 1-2, p. 22-41, 2004.

HIDE, Geoff. Role of vertical transmission of Toxoplasma gondii in prevalence of infection. Expert review of anti-infective therapy, v. 14, n. 3, p. 335-344, 2016.

KOVAL, Michael; MOLINA, Samuel A.; BURT, Janis M. Mix and match: investigating heteromeric and heterotypic gap junction channels in model systems and native tissues. FEBS letters, v. 588, n. 8, p. 1193-1204, 2014.

KUMAR, Nalin M.; GILULA, Norton B. The gap junction communication channel. Cell, v. 84, n. 3, p. 381-388, 1996.

LILLY, Evelyn et al. Connexin channels in congenital skin disorders. In: Seminars in cell & developmental biology. Academic Press, 2016. p. 4-12.

LÜDER, Carsten GK; BOHNE, Wolfgang; SOLDATI, Dominique. Toxoplasmosis: a persisting challenge. Trends in parasitology, v. 17, n. 10, p. 460-463, 2001.

MONTECINO-RODRIGUEZ, Encarnacion; LEATHERS, Hyosuk; DORSHKIND, Kenneth. Expression of connexin 43 (Cx43) is critical for normal hematopoiesis. Blood, v. 96, n. 3, p. 917-924, 2000.

MUSIL, Linda S.; GOODENOUGH, Daniel A. Multisubunit assembly of an integral plasma membrane channel protein, gap junction connexin43, occurs after exit from the ER. Cell, v. 74, n. 6, p. 1065-1077, 1993.

NEIJSSEN, Joost et al. Cross-presentation by intercellular peptide transfer through gap junctions. Nature, v. 434, n. 7029, p. 83, 2005.

NEIJSSEN, Joost; PANG, Baoxu; NEEFJES, Jacques. Gap junction-mediated intercellular communication in the immune system. Progress in biophysics and molecular biology, v. 94, n. 1-2, p. 207-218, 2007.

NOGUEIRA, Alanderson R. et al. Effect of Toxoplasma gondii infection on the junctional complex of retinal pigment epithelial cells. Parasitology, v. 143, n. 5, p. 568-575, 2016.

REAUME, Andrew G. et al. Cardiac malformation in neonatal mice lacking connexin43. Science, v. 267, n. 5205, p. 1831-1834, 1995.

SEABRA, Sergio H.; DE SOUZA, Wanderley; DAMATTA, Renato A. Toxoplasma gondii partially inhibits nitric oxide production of activated murine macrophages. Experimental parasitology, v. 100, n. 1, p. 62-70, 2002.

SEABRA, Sergio H.; DE SOUZA, Wanderley; DAMATTA, Renato A. Toxoplasma gondii exposes phosphatidylserine inducing a TGF-?1 autocrine effect orchestrating macrophage evasion. Biochemical and biophysical research communications, v. 324, n. 2, p. 744-752, 2004.

SEGRETAIN, Dominique; FALK, Matthias M. Regulation of connexin biosynthesis, assembly, gap junction formation, and removal. Biochimica et Biophysica Acta (BBA)-Biomembranes, v. 1662, n. 1-2, p. 3-21, 2004.

VEGA, Virginia L.; DE MAIO, Antonio. Geldanamycin treatment ameliorates the response to LPS in murine macrophages by decreasing CD14 surface expression. Molecular biology of the cell, v. 14, n. 2, p. 764-773, 2003.

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Published

2021-01-20

How to Cite

de Carvalho, G. O. A. M., Souza, O. M. de J., Souza, D. R., de Souza, R. do N., de Mello, T. M., Goldenberg, R. C. dos S., Seabra, S. H., & Fortes, F. da S. de A. (2021). Junction communication in the immune system: modulation of the GAP junctions by infection with Toxoplasma gondii / Comunicação juncional no sistema imunológico: modulação das junções GAP em infecção por Toxoplasma gondii. Brazilian Journal of Development, 7(1), 4165–4182. https://doi.org/10.34117/bjdv7n1-281

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Original Papers