Transferência horizontal de genes mediada pelo plasmídeo pCF10 em Enterococcus faecalis: impacto sobre a disseminação de linhagens multirresistentes e evolução da virulência / Horizontal gene transfer mediated by pCF10 plasmid in Enterococcus faecalis: Impact on the spread of multidrug resistant strains and evolution of virulence

Authors

  • Elias Ribeiro
  • Fabiana Brandão
  • Tanise Vendruscolo Dalmolin

DOI:

https://doi.org/10.34117/bjdv7n1-141

Keywords:

Plasmídeo responsivo a feromônio, pCF10, E. faecalis, resistência, virulência, transferência horizontal.

Abstract

Introdução: E. faecalis é uma bactéria Gram-positiva multirresistente a antibióticos que possui uma vasta capacidade de transferir genes de resistência, em grande parte através da conjugação de plasmídeos responsívos a feromônios, sendo o pCF10 um dos mais estudados até o momento.Objetivo: Analisar a iniciação da conjugação mediada pelo plasmídeo pCF10, bem como os sistemas de regulação do plasmídeo e implicações fenotípicas da transferência de genes relacionados ao aumento da virulência e resistência a antibióticos em E. faecalis. Conclusão: O plasmídeo pCF10 pode carrear genes que codificam resistência a antibióticos e fatores de virulência intra e inter espécies bacterianas, particularmente, elevando o  risco de infecções por E. faecalis e a problemática do surgimento de micro-organismos multirresistente.

References

A ARIAS, Cesar; MURRAY, Barbara e. Emergence and management of drug-resistant enterococcal infections. Expert Review Of Anti-Infective Therapy, [S.L.], v. 6, n. 5, p. 637-655, out. 2008. Informa UK Limited. http://dx.doi.org/10.1586/14787210.6.5.63

HIGUITA, Nelson I. Agudelo; HUYCKE, Mark M; IKE, Yasuyoshi; SHANKAR, Nathan. Enterococcal Disease, Epidemiology, and Implications for Treatment: enterococcal disease. In: GILMORE, Michael s et al. Enterococci: from commensals to leading causes of drug resistant infection. Boston: Massachusetts Eye And Ear Infirmary Boston, 2014. Cap. 2. p. 65-68. Disponível em: https://pubmed.ncbi.nlm.nih.gov/24649510/. Acesso em: 30 de Novembro 2020.

HOLLENBECK, Brian L.; RICE, Louis B.. Intrinsic and acquired resistance mechanisms in enterococcus. Virulence, [S.L.], v. 3, n. 5, p. 421-569, 15 ago. 2012. Informa UK Limited. http://dx.doi.org/10.4161/viru.21282

CRANK, Christopher; O'DRISCOLL, Tristan. Vancomycin-resistant enterococcal infections: epidemiology, clinical manifestations, and optimal management. Infection And Drug Resistance, [S.L.], p. 217-230, jul. 2015. Informa UK Limited. http://dx.doi.org/10.2147/idr.s54125

BENDER, Jennifer K.; CATTOIR, Vincent; HEGSTAD, Kristin; SADOWY, Ewa; COQUE, Teresa M.; WESTH, Henrik; HAMMERUM, Anette M.; SCHAFFER, Kirsten; BURNS, Karen; MURCHAN, Stephen. Update on prevalence and mechanisms of resistance to linezolid, tigecycline and daptomycin in enterococci in Europe: towards a common nomenclature. Drug Resistance Updates, [S.L.], v. 40, p. 25-39, set. 2018. Elsevier BV. http://dx.doi.org/10.1016/j.drup.2018.10.002.

MCINNES, Ross s; MCCALLUM, Gregory e; LAMBERTE, Lisa e; VAN SCHAIK, Willem. Horizontal transfer of antibiotic resistance genes in the human gut microbiome. Current Opinion In Microbiology, [S.L.], v. 53, p. 35-43, fev. 2020. Elsevier BV. http://dx.doi.org/10.1016/j.mib.2020.02.002

HUDDLESTON, Jennifer R. Horizontal gene transfer in the human gastrointestinal tract: potential spread of antibiotic resistance genes. Infection And Drug Resistance, [S.L.], p. 167-176, jun. 2014. Informa UK Limited. http://dx.doi.org/10.2147/idr.s48820

DUNNY, Gary M. The peptide pheromone-inducible conjugation system of Enterococcus faecalis plasmid pCF10: cell?cell signalling, gene transfer, complexity and evolution. Philosophical Transactions Of The Royal Society B: Biological Sciences, [S.L.], v. 362, n. 1483, p. 1185-1193, 13 mar. 2007. The Royal Society. http://dx.doi.org/10.1098/rstb.2007.2043

ANTIPORTA, Michelle H.; DUNNY, Gary M.. CcfA, the Genetic Determinant for the cCF10 Peptide Pheromone in Enterococcus faecalis OG1RF. Journal Of Bacteriology, [S.L.], v. 184, n. 4, p. 1155-1162, 15 fev. 2002. American Society for Microbiology. http://dx.doi.org/10.1128/jb.184.4.1155-1162.2002

PALMER, Kelli L; KOS, Veronica N; GILMORE, Michael s. Horizontal gene transfer and the genomics of enterococcal antibiotic resistance. Current Opinion In Microbiology, [S.L.], v. 13, n. 5, p. 632-639, out. 2010. Elsevier BV. http://dx.doi.org/10.1016/j.mib.2010.08.004

CHANDLER, Josephine R.; FLYNN, Aron R.; BRYAN, Edward M.; DUNNY, Gary M.. Specific Control of Endogenous cCF10 Pheromone by a Conserved Domain of the pCF10-Encoded Regulatory Protein PrgY in Enterococcus faecalis. Journal Of Bacteriology, [S.L.], v. 187, n. 14, p. 4830-4843, jul. 2005. American Society for Microbiology. http://dx.doi.org/10.1128/jb.187.14.4830-4843.2005

NAKAYAMA, J; RUHFEL, R e; DUNNY, G M; A ISOGAI,; A SUZUKI,. The prgQ gene of the Enterococcus faecalis tetracycline resistance plasmid pCF10 encodes a peptide inhibitor, iCF10. Journal Of Bacteriology, [S.L.], v. 176, n. 23, p. 7405-7408, 1994. American Society for Microbiology. http://dx.doi.org/10.1128/jb.176.23.7405-7408.1994

SHI, K.; BROWN, C. K.; GU, Z.-Y.; KOZLOWICZ, B. K.; DUNNY, G. M.; OHLENDORF, D. H.; EARHART, C. A.. Structure of peptide sex pheromone receptor PrgX and PrgX/pheromone complexes and regulation of conjugation in Enterococcus faecalis. Proceedings Of The National Academy Of Sciences, [S.L.], v. 102, n. 51, p. 18596-18601, 8 dez. 2005. Proceedings of the National Academy of Sciences. http://dx.doi.org/10.1073/pnas.0506163102

CHATTERJEE, A.; JOHNSON, C. M.; SHU, C.-C.; KAZNESSIS, Y. N.; RAMKRISHNA, D.; DUNNY, G. M.; HU, W.-S.. Convergent transcription confers a bistable switch in Enterococcus faecalis conjugation. Proceedings Of The National Academy Of Sciences, [S.L.], v. 108, n. 23, p. 9721-9726, 23 maio 2011. Proceedings of the National Academy of Sciences. http://dx.doi.org/10.1073/pnas.1101569108

CHATTERJEE, A.; COOK, L. C. C.; SHU, C.-C.; CHEN, Y.; MANIAS, D. A.; RAMKRISHNA, D.; DUNNY, G. M.; HU, W.-S.. Antagonistic self-sensing and mate-sensing signaling controls antibiotic-resistance transfer. Proceedings Of The National Academy Of Sciences, [S.L.], v. 110, n. 17, p. 7086-7090, 8 abr. 2013. Proceedings of the National Academy of Sciences. http://dx.doi.org/10.1073/pnas.1212256110

BANDYOPADHYAY, Arpan; O'BRIEN, Sofie; FRANK, Kristi L.; DUNNY, Gary M.; HU, Wei-Shou. Antagonistic Donor Density Effect Conserved in Multiple Enterococcal Conjugative Plasmids. Applied And Environmental Microbiology, [S.L.], v. 82, n. 15, p. 4537-4545, 20 maio 2016. American Society for Microbiology. http://dx.doi.org/10.1128/aem.00363-16.

HEGSTAD, K.; MIKALSEN, T.; COQUE, T.M.; WERNER, G.; SUNDSFJORD, A.. Mobile genetic elements and their contribution to the emergence of antimicrobial resistant Enterococcus faecalis and Enterococcus faecium. Clinical Microbiology And Infection, [S.L.], v. 16, n. 6, p. 541-554, jun. 2010. Elsevier BV. http://dx.doi.org/10.1111/j.1469-0691.2010.03226.x

HIRT, Helmut; SCHLIEVERT, Patrick M.; DUNNY, Gary M.. In Vivo Induction of Virulence and Antibiotic Resistance Transfer in Enterococcus faecalis Mediated by the Sex Pheromone-Sensing System of pCF10. Infection And Immunity, [S.L.], v. 70, n. 2, p. 716-723, fev. 2002. American Society for Microbiology. http://dx.doi.org/10.1128/iai.70.2.716-723.2002

STERLING, Amy J.; SNELLING, William J.; NAUGHTON, Patrick J.; TERNAN, Nigel G.; DOOLEY, James S. G.. Competent but complex communication: the phenomena of pheromone-responsive plasmids. Plos Pathogens, [S.L.], v. 16, n. 4, p. 1-19, 2 abr. 2020. Public Library of Science (PLoS). http://dx.doi.org/10.1371/journal.ppat.1008310

CHOI, Jong-Mi; WOO, Gun-Jo. Transfer of Tetracycline Resistance Genes with Aggregation Substance in Food-Borne Enterococcus faecalis. Current Microbiology, [S.L.], v. 70, n. 4, p. 476-484, 7 dez. 2014. Springer Science and Business Media LLC. http://dx.doi.org/10.1007/s00284-014-0742-1

KIM, Yeong Bin; SEO, Kwang Won; JEON, Hye Young; LIM, Suk-Kyung; SUNG, Haan Woo; LEE, Young Ju. Molecular characterization of erythromycin and tetracycline-resistant Enterococcus faecalis isolated from retail chicken meats. Poultry Science, [S.L.], v. 98, n. 2, p. 977-983, fev. 2019. Elsevier BV. http://dx.doi.org/10.3382/ps/pey477

TREMBLAY, Cindy-Love; ARCHAMBAULT, Marie. Interference in Pheromone-Responsive Conjugation of a High-Level Bacitracin Resistant Enterococcus faecalis Plasmid of Poultry Origin. International Journal Of Environmental Research And Public Health, [S.L.], v. 10, n. 9, p. 4245-4260, 11 set. 2013. MDPI AG. http://dx.doi.org/10.3390/ijerph10094245

LIM, Suk-Kyung; TANIMOTO, Koichi; TOMITA, Haruyoshi; IKE, Yasuyoshi. Pheromone-Responsive Conjugative Vancomycin Resistance Plasmids in Enterococcus faecalis Isolates from Humans and Chicken Feces. Applied And Environmental Microbiology, [S.L.], v. 72, n. 10, p. 6544-6553, out. 2006. American Society for Microbiology. http://dx.doi.org/10.1128/aem.00749-06

ZHENG, Bo; TOMITA, Haruyoshi; INOUE, Takako; IKE, Yasuyoshi. Isolation of VanB-Type Enterococcus faecalis Strains from Nosocomial Infections: first report of the isolation and identification of the pheromone-responsive plasmids pmg2200, encoding vanb-type vancomycin resistance and a bac41-type bacteriocin, and pmg2201, encoding erythromycin resistance and cytolysin (hly/bac). Antimicrobial Agents And Chemotherapy, [S.L.], v. 53, n. 2, p. 735-747, 24 nov. 2008. American Society for Microbiology. http://dx.doi.org/10.1128/aac.00754-08

BREUER, Rebecca J.; HIRT, Helmut; DUNNY, Gary M.. Mechanistic Features of the Enterococcal pCF10 Sex Pheromone Response and the Biology ofEnterococcus faecalisin Its Natural Habitat. Journal Of Bacteriology, [S.L.], v. 200, n. 14, p. 1-9, 5 fev. 2018. American Society for Microbiology. http://dx.doi.org/10.1128/jb.00733-17

MADSEN, Kristian T.; SKOV, Marianne N.; GILL, Sabine; KEMP, Michael. Virulence Factors Associated with Enterococcus Faecalis Infective Endocarditis: a mini review. The Open Microbiology Journal, [S.L.], v. 11, n. 1, p. 1-11, 31 mar. 2017. Bentham Science Publishers Ltd.. http://dx.doi.org/10.2174/1874285801711010001

BHATTY, Minny; CAMACHO, Martha I.; GONZALEZ?RIVERA, Christian; FRANK, Kristi L.; DALE, Jennifer L.; MANIAS, Dawn A.; DUNNY, Gary M.; CHRISTIE, Peter J.. PrgU: a suppressor of sex pheromone toxicity inenterococcus faecalis. Molecular Microbiology, [S.L.], v. 103, n. 3, p. 398-412, 16 dez. 2016. Wiley. http://dx.doi.org/10.1111/mmi.13563

Published

2021-01-20

How to Cite

Ribeiro, E., Brandão, F., & Dalmolin, T. V. (2021). Transferência horizontal de genes mediada pelo plasmídeo pCF10 em Enterococcus faecalis: impacto sobre a disseminação de linhagens multirresistentes e evolução da virulência / Horizontal gene transfer mediated by pCF10 plasmid in Enterococcus faecalis: Impact on the spread of multidrug resistant strains and evolution of virulence. Brazilian Journal of Development, 7(1), 2066–2077. https://doi.org/10.34117/bjdv7n1-141

Issue

Section

Original Papers