In vitro characterization of endophytic bacteria associated with physic nut (Jatropha curcas L.) and their potential for plant-growth promotion and biocontrol / Caracterização in vitro de bactérias endofíticas associadas ao pinhão-manso (Jatropha curcas L.) e seu potencial de promoção de crescimento vegetal e biocontrole

Paula Cristiane Machado, Paulo Henrique Marques Andrade, Cristina Paiva de Sousa, Clovis Wesley Oliveira de Souza, Paulo Teixeira Lacava

Abstract


The physic nut (Jatropha curcas L.) is a shrubby plant of perennial cycle, belonging to the family Euphorbiaceae, from Central America and currently vegetates spontaneously in diverse regions of the planet. The commercial interest in Brazil occurred due to the desirable characteristics of that crop as an agricultural option for renewal of the Brazilian energy base, being a promising raw material for biodiesel production. Oil plants that have a high biotechnological potential may have a genetically diverse microbial population with characteristics of promoting the growth of multifunctional plants. Plant growth-promoting endophytes (PGPE) are of biotechnological interest since they can improve the growth of several important agronomical crops. The present study aimed the biochemistry characterization of thirty-seven endophytic bacteria strains associated with J. curcas plants, with the potential of plant growth promotion. Of this total of evaluated strains, 75% showed positive results for fixation of nitrogen, 62% produced IAA in the presence of the tryptophan precursor, 32% solubilized inorganic phosphate and 35% exhibited antagonistic activities against phytopathogenic fungi (Lasiodiplodia. subglobosa, L. euphorbicola, and L. pseudotheobromae) in physic nut. To our knowledge, this is the first report of this potential of biocontrol against Lasiodiplodia species. Among the thirty-seven bacterial isolates identified by partial sequencing of the 16S gene, the presence of the genera Arthrobacter, Bacillus, Citrobacter, Curtobacterium, Enterococcus, Klebsiella, Leucobacter, Lysinibacillus, Microbacterium, Rhodococcus, and Serratia was observed. Our results indicated that the cultivable endophytic bacteria isolated from J. curcas have the potential to demonstrate multiple characteristics of PGPE in vitro and have the potential for other large-scale assays such as biofertilizer and biopesticides.

 

 


Keywords


Biofertilizers, Plant growth promotion, Sustainable agriculture.

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DOI: https://doi.org/10.34117/bjdv6n11-326

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