Caracterização da enzima Oxidase Alternativa do fungo Moniliophthora perniciosa com o auxilio das ferramentas de bioinformática / Characterization of the enzyme Oxidase Alternative of the fungus Moniliophthora perniciosa with the aid of bioinformatics tools

Renata dos Santos Santana, Eduardo Almeida Costa, Sônia Cristina Oliveira Melo, Cristina Pungartnik

Abstract


Moniliophthora perniciosa (Mp) é um fungo basidiomiceto, fitopatogênico, causador da doença vassoura de bruxa no cacau (Theobroma cacao L.), apresenta duas fases no ciclo de vida: a tipo biotrófica (nos tecidos vivos do hospedeiro), e fase saprofítica (na qual ocorre a morte da planta). A vassoura de bruxa é uma doença responsável por causar grandes perdas na produção de cacau. Estudos anteriores mostram que o Mp apresenta a enzima oxidase alternativa (AOX), a qual está relacionada com a virulência e resistência a fungicidas. Quando submetido a situações de estresse, pincipalmente quando há comprometimento da via principal de respiração, a AOX apresenta uma via respiratória alternativa como mecanismo de sobrevivência. Em nosso trabalho, foi realizada a caracterização da enzima AOX de Mp comparada com a AOX de outros basidiomicetos, utilizando ferramentas de bioinformática, com o intuito de conhecer melhor essa enzima que proporciona sobrevivência a este fungo. Concluimos que a AOX do Mp compartilha alta similaridade principalmente com AOX do Moniliophthora roreri, desempenhando funções semelhantes como a via respiratória alternativa, quando estes microrganismos são submetidos a situações de estresse.

 

 


Keywords


Moniliophthora perniciosa, Moniliophthora roreri, oxidase alternativa, basidiomicetos, vassoura de bruxa.

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DOI: https://doi.org/10.34117/bjdv6n10-533

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