Production of substrates for determination of xylanase activity / Produção de substratos para determinação da actividade da xilanase

Renato Henrique Calore, Danilo Bueno, Michel Brienzo

Abstract


The hemicelulose is a molecule heterogeneous and, therefore, its extraction from different biomass is a challenge. In this context this study proposed an hemicelulose extraction from sugarcane biomass using alkaline pretreatment to determination of xylanase activities and evaluation of affinity between substrates and enzymes. The results suggest that the xylan from sugarcane leaves showed greater affinity with the enzymes, but the xylan from bagasse was with more efficient and pure extraction.

 


Keywords


hemicelullose; biorrefinering; biomass; xylanolitic enzymes.

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References


BAILEY, M.J.; BIELY, P.; POUTANEN, K. Interlaboratory testing of methods for assay of xylanase activity. Journal of Biotechnology, v. 23, p. 257-270, 1992.

BERNAL, H.G.; BERNAZZANI, L.; GALLETTI, A.M.R. Furfural from corn stover hemicelluloses. A mineral acid-free approach. Green Chemistry, v. 1, p. 1-7, 2014.

BRIENZO, M.; SIQUEIRA, A.F.; MILAGRES, A.M.F. Search for optimum conditions of sugarcane bagasse hemicellulose extraction. Biochemical Engineering Journal, v. 46, p. 199-204, 2009.

BRIENZO, M.; CARVALHO, W.; MILAGRES, A.M.F. Xylooligosaccharides Production from Alkali-Pretreated Sugarcane Bagasse Using Xylanases from Thermoascus aurantiacus. Applied Biochemistry and Biotechnology, v. 162, p. 1195-1205, 2010.

Brienzo, M.; CARVALHO, A. F. A. ; FIGUEIREDO, F. C. ; OLIVA NETO, P. . Sugarcane bagasse hemicellulose properties, extraction technologies and xylooligosaccharides production. Food Waste: Practices, Management and Challenges. 1ed.New York: Nova Science Publishers, 2016, v. , p. 155-188.

CORREDOR, D.Y.; SUN, X.S.; SALAZAR, J.M.; HOHN, K.L.; WANG, D. Enzymatic Hydrolysis of Soybean Hulls Using Dilute Acid and Modified Steam-Explosion Pretreatments. Journal of Biobased Materials and Bioenergy, v. 2, p. 43-50, 2008.

FREITAS. C.; CARMONA, E.; BRIENZO, M. Xylooligosaccharides production process from lignocellulosic biomass and bioactive effects. Bioactive Carbohydrates and Dietary Fibre, v. 18, p. 1-8, 2019.

GOUVEIA, Ester Ribeiro et al. Validação de metodologia para a caracterização química de bagaço de cana-de-açúcar. Química Nova, v. 32, n. 6, p. 1500-1503, 2009.

MILLER, G. L. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry, v. 31, p. 426-428, 1959.

NEILANDS, J.B.; STUMPF, P.K. Outlines of enzyme chemistry. Nova Iorque: Wiley and Sons, Inc., 1955.

PURCHASE, B. S. et al. Products from sugarcane. International sugar journal, v. 97, n. 1154, p. 70-81, 1995.

ROCHA, M.S.R.S.; PRATTO, B.; JÚNIOR, R.S.; ALMEIDA, R.M.R..G.; CRUZ, A.J.G. A kinetic model for hydrothermal pretreatment of sugarcane straw. Bioresource Technology, v. 228, p. 176-185, 2017.

WANG, S.; RU, B.; LIN, H.; SUN, W. Pyrolysis behaviors of four O-acetyl-preserved hemicelluloses isolated from hardwoods and softwoods. Fuel, v. 150, p. 243-251, 2015.




DOI: https://doi.org/10.34117/bjdv6n10-318

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