Production of substrates for determination of xylanase activity / Produção de substratos para determinação da actividade da xilanase

Renato Henrique Calore, Danilo Bueno, Michel Brienzo


The hemicelulose is a molecule heterogeneous and, therefore, its extraction from different biomass is a challenge. In this context this study proposed an hemicelulose extraction from sugarcane biomass using alkaline pretreatment to determination of xylanase activities and evaluation of affinity between substrates and enzymes. The results suggest that the xylan from sugarcane leaves showed greater affinity with the enzymes, but the xylan from bagasse was with more efficient and pure extraction.



hemicelullose; biorrefinering; biomass; xylanolitic enzymes.

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