Antibacterial and antibiofilm activity of Physalis peruviana calyx extract / Atividade antibacteriana e antibiofilme do extrato do cálice de Physalis peruviana

Maiara Taís Bazana, Marcos Vinícius Missel, Cassandra de Deus, Vandré Sonza Pinto, Cristiane Franco Codevilla, Roberto Christ Vianna Santos, Cristiane de Bona da Silva, Cristiano Ragagnin de Menezes

Abstract


This study aimed to evaluate the antibacterial and antibiofilm activity of a P. peruviana calyx extract. Goldenberry calyx extracts were prepared with 60% (v/v) ethanol. Minimum inhibitory concentration (MIC) analyses were performed by the 96-well plate microdilution method together with the minimum bactericidal concentration (MBC). Biofilm inhibition and destruction was performed in microdilution plates. The P. peruviana calyx extract presented antibacterial activity against the pathogens analyzed (Enterobacter aerogenes ATCC 13048, Klebsiella pneumoniae ATCC 1705, Staphylococcus epidermidis (clinical isolate), Streptococcus pneumoniae ATCC 99619, Pseudomonas aeruginosa PA01, Enterococcus faecalis ATCC 29212, Escherichia coli (clinical isolate), Shigella sonnei (clinical isolate), Acinetobacter baumannii ATCC 19606, Streptococcus agalactie (clinical isolate), Acinetobacter baumannii (clinical isolate), Salmonella sp. (clinical isolate), Salmonella enteritidis (clinical isolate), and Staphylococcus aureus (clinical isolate)). MIC varied from 3.15 to 30 mg/mL extract and showed bacteriostatic activity against eight pathogens and bactericidal activity at 30 mg/mL concentration against six strains. Biofilm tests revealed biofilm formation inhibition, although there was no destruction. According to these results, the potential antibacterial activity of P. peruviana calyx extract was verified. This will enable further studies to be carried out to contribute to its use in the food industry as a preservative of natural origin and other clinical applications.

Keywords


biofilm, goldenberry, minimum inhibitory concentration, minimum bactericidal concentration.

Full Text:

PDF

References


Ö. Ertürk, M. Çol Ayvaz, Z. Can, Ü. Karaman, K. Korkmaz, Antioxidant, antimicrobial activities and phenolic and chemical contents of Physalis peruviana L. from Trabzon, Turkey, Indian J. Pharm. Educ. Res. 51 (2017) S213–S216. https://doi.org/10.5530/ijper.51.3s.15.

F. Göztok, F. Zengin, The antimicrobial activity of Physalis peruviana L., Bitlis Eren Univ. J. Sci. Technol. 3 (2013) 15–17. https://doi.org/10.17678/beujst.51277.

P.K. Kamau, Z. Ng’ang’a, P. Gakio, F.M. Njeruh, J. Thuita, Antimicrobial evaluation and phytochemical screening of aqueous and dichloromethane crude extracts of Kenyan Physalis peruviana L ( Cape Gooseberry ), 16 (2017) 101–109. https://doi.org/10.9790/0853-160509101109.

M.B.R. Cueva, R.A. Tigre León, M.M.L. Angel Yanchaliquín, I.F.B.M. Herminia Sanaguano Salguero, Antibacterial Effects of Uvilla (Physalis peruviana L.) extracts against Listeria spp. Isolated from Meat in Ecuador, Int. J. Curr. Microbiol. Appl. Sci. 6 (2017) 1146–1153. https://doi.org/10.20546/ijcmas.2017.604.141.

S. Medina, J. Collado-González, F. Ferreres, J. Londoño-Londoño, C. Jiménez-Cartagena, A. Guy, T. Durand, J.M. Galano, Á. Gil-Izquierdo, Potential of Physalis peruviana calyces as a low-cost valuable resource of phytoprostanes and phenolic compounds, J. Sci. Food Agric. 99 (2019) 2194–2204. https://doi.org/10.1002/jsfa.9413.

WHO publishes list of bacteria for which new antibiotics are urgently needed, World Heal. Organ. (2017). https://www.who.int/news-room/detail/27-02-2017-who-publishes-list-of-bacteria-for-which-new-antibiotics-are-urgently-needed (accessed April 13, 2020).

L. R. Trabulsi, F. Alterthum, Microbiologia, sixth ed., Atheneu, São Paulo, 2015.

S.J. Forsythe, Microbiologia da segurança dos alimentos, second ed., Artmed, Porto Alegre, 2013.

J.L. da C. Lima, L.R. Alves, P.R.L. de A. Jacomé, J.P. Bezerra Neto, M.A.V. Maciel, M.M.C. de Morais, Biofilm production by clinical isolates of Pseudomonas aeruginosa and structural changes in LasR protein of isolates non biofilm-producing, Brazilian J. Infect. Dis. 22 (2018) 129–136. https://doi.org/10.1016/j.bjid.2018.03.003.

J. Bai, Y.T. Kim, S. Ryu, J.H. Lee, Biocontrol and rapid detection of food-borne pathogens using bacteriophages and endolysins, Front. Microbiol. 7 (2016) 1–15. https://doi.org/10.3389/fmicb.2016.00474.

K.H. Lee, J.S. Lee, E.S. Kim, H.G. Lee, Preparation, characterization, and food application of rosemary extract-loaded antimicrobial nanoparticle dispersions, LWT. 101 (2019) 138–144. https://doi.org/10.1016/j.lwt.2018.10.072.

R.C. Baptista, C.N. Horita, A.S.S. Ana, Natural products with preservative properties for enhancing the microbiological safety and extending the shelf-life of seafood: a review, Food Res. Int. 127 (2019) 108762. https://doi.org/10.1016/j.foodres.2019.108762.

H. Antolak, G. Kregiel, Food preservatives from plants, in: Food Addit., 2017: pp. 45–48. https://doi.org/10.5772/intechopen.70090.

CLSI, Performance standards for antimicrobial susceptibility testing. 27th ed. CLSI supplement M100. Wayne, PA: Clinical and Laboratory Standards Institute, 2017.

M.T. Bazana, S.S. da Silva, C.F. Codevilla, C. de Deus, B.N. Lucas, G.A. Ugalde, M.A. Mazutti, E.M. Moraes Flores, J.S. Barin, C. de Bona da Silva, C.R. de Menezes, Development of nanoemulsions containing Physalis peruviana calyx extract: A study on stability and antioxidant capacity, Food Res. Int. 125 (2019) 108645. https://doi.org/10.1016/j.foodres.2019.108645.

P. Courvalin, L’ant biogramme, Paris, 1985.

P.C. Bonez, G.G. Rossi, J.R. Bandeira, A.P. Ramos, C.R. Mizdal, V.A. Agertt, E.S.S. Dalla Nora, M.E. de Souza, C.F. dos Santos Alves, F.S. dos Santos, A. Gündel, R. de Almeida Vaucher, R.C.V. Santos, M.M.A. de Campos, Anti-biofilm activity of A22 ((S-3,4-dichlorobenzyl) isothiourea hydrochloride) against Pseudomonas aeruginosa: Influence on biofilm formation, motility and bioadhesion, Microb. Pathog. 111 (2017) 6–13. https://doi.org/10.1016/j.micpath.2017.08.008.

D. Ballesteros-Vivas, G. Álvarez-Rivera, E. Ibáñez, F. Parada-Alfonso, A. Cifuentes, A multi-analytical platform based on pressurized-liquid extraction, in vitro assays and liquid chromatography/gas chromatography coupled to high resolution mass spectrometry for food by-products valorisation. Part 2: Characterization of bioactive compound, J. Chromatogr. A. 1584 (2019) 144–154. https://doi.org/10.1016/j.chroma.2018.11.054.

L.A. Puente, C.A. Pinto-Muñoz, E.S. Castro, M. Cortés, Physalis peruviana Linnaeus, the multiple properties of a highly functional fruit: A review, Food Res. Int. 44 (2011) 1733–1740. https://doi.org/10.1016/j.foodres.2010.09.034.

M.F. Moradali, S. Ghods, B.H.A. Rehm, Pseudomonas aeruginosa Lifestyle: A Paradigm for Adaptation, Survival, and Persistence, Front. Cell. Infect. Microbiol. 7 (2017) 1–29. https://doi.org/10.3389/fcimb.2017.00039.

A. Meliani, A. Bensoltane, Review of Pseudomonas Attachment and Biofilm Formation in Food Industry, Poultry, Fish. Wildl. Sci. 03 (2016) 1–7. https://doi.org/10.4172/2375-446x.1000126.

B.L. Crippa, M.R.Z. Elgui, P.E. Budri, I.G. Castilho, Microbiological evaluation of minimally processed foods. Genotypic and phenotypic profile of Staphylococcus sp strains, regarding the production of biofilm and enterotoxins, Braz. J. of Develop.6(9) (2020) 65789-65804. https://www.brazilianjournals.com/index.php/BRJD/article/view/16240

L.C.P. Silva, J.V.L. Silva, T.S.S. Melo, D.R.S. Silva, D.G. Costa, T.C.L. Vasconcelos, Análise in vitro da atividade antimicrobiana do extrato de Vaccinium Macrocarpon (Cranberry) e óleo essencial de Origanum vulgare (Orégano) frente à cepa de Escherichia coli, Braz. J. of Develop. 6(9) (2020) 70057-70069. https://www.brazilianjournals.com/index.php/BRJD/article/view/16893/13777

J.O.S. Vargas, R.Reis, G. Maldaner, P.A. Mariño, A.P.S. Menezes, Avaliação do potencial antibacteriano e antifúngico de Maytenus ilicifolia (Mart. ex Reissek) oriunda da região do Bioma Pampa, Braz. J. of Develop. 6(9) (2020) 66364-66376. https://www.brazilianjournals.com/index.php/BRJD/article/view/16316/13343.




DOI: https://doi.org/10.34117/bjdv6n10-245

Refbacks

  • There are currently no refbacks.