Microbial content recovered from diabetic foot infections: a cross-sectional study in Brazil / Conteúdo microbiano recuperado em infecções de pé diabético: um estudo transversal no Brasil

Renata Barreto Francisco, Cristina Dutra Vieira, Luiz de Macêdo Farias, Antônio Eustáquio de Souza, Bruno Lima de Castro, Renato Rivail Rodrigues Braga, Danielle Alves Gomes Zauli, Maria Auxiliadora Roque de Carvalho, Simone Gonçalves dos Santos

Abstract


In Brazil, the prevalence of diabetes mellitus (DM) is 11.9 million cases. Diabetic foot ulcers (DFU) increase morbidity and cause hospital admissions among DM patients. In an attempt to better understand DFU, this cross-sectional study investigated microbial content and their susceptibility to antimicrobials. Secretion from foot ulcers of 30 diabetic patients were obtained in three Brazilian hospitals and submitted to microbiological evaluation. All recovered strains were identified and submitted to antimicrobial susceptibility tests. Genetic diversity was investigated by PCR coupled with denaturing gradient gel electrophoresis (PCR-DGGE). DFU exhibited a polymicrobial profile composed of 72.5% aerobic and 22.3% anaerobic bacteria, and 2.5% fungi species. A total of 91 microorganisms were isolated, and the number of recovered species per patient ranged from 1-9. Peptostreptococcus spp. was the most frequently recovered obligate anaerobic Genus and was resistant mostly to penicillin and clindamycin. A total of 37.5% S. aureus strains were methicillin resistant. E. coli were the most susceptible Gram-negative species and Pseudomonas aeruginosa were the most resistant. The present study demonstrated that almost 34% of microbial species observed on DGGE gels were not cultivable. The recovery of multidrug resistant microorganisms pointed out to the need for more attention when prescribing an empirical therapy and emphasized the relevance of this study.


Keywords


Diabetes mellitus, diabetic foot ulcers, polymicrobial infections, antimicrobial susceptibility, genetic testing.

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References


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DOI: https://doi.org/10.34117/bjdv6n10-155

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