Antioxidant and antimicrobial properties of eucalyptus leaf extract obtained using pressurized ethanol / Propriedades antioxidantes e antimicrobianas do extrato de folhas de eucalipto obtidos com etanol pressurizado

Bruno Fischer, Eloir Pereira Gowzdz, Giovana Feltes, Patrícia Griep, Alexander Junges, Natalia Paroul, Rogério Luis Cansian

Abstract


Eucalyptus leaves are a secondary product of wood production. The pressurized liquid extraction (PLE) technique is an attractive process due to its advantages. The objective of this work was to obtain extracts of Eucalyptus saligna leaves using the PLE technique, and to characterize different extraction time fractions in relation to bioactive compounds and antioxidant and antimicrobial activities. The extraction of E. saligna leaves was carried out in a jacketed extractor at 40 °C, pressure of 10.4 MPa, with a solvent flow of 2.5 mL.min-1, using 95% ethanol. The system remained pressurized for 30 minutes in static extraction, followed by 180 minutes in dynamic extraction. The levels of total phenols, flavonoids, antioxidant activity and antimicrobial potential against Staphylococcus aureus and Escherichia coli were evaluated. The extraction showed an overall yield of 12.3% after 180 minutes. The total concentrations of phenols and flavonoids were 618.57 mgGAE.g-1 and 160.28 mgQE.g-1 respectively. The extracts showed good antioxidant and antimicrobial potential, with IC50 between 0.016 and 0.043 mg.mL-1, and MIC against S. aureus and E. coli between 0.313 and 1.25 mg.mL-1. PLE demonstrated efficiency in obtaining extracts of E. saligna leaves with bioactive properties.


Keywords


Eucalyptus saligna, bioactive properties, phenolic compounds, pressurized liquid extraction.

References


ANDREWS, J. M. Determinação de concentrações inibitórias mínimas. Jornal de quimioterapia antimicrobiana. v.48 n. suppl 1, p.5-16, 2001. doi: 10.1093/jac/48.suppl_1.5

ANTUNES, T. V.; FREITAS, R. F.; & ROYO, V. A. Avaliação da atividade antimicrobiana do extrato hidroalcoólico das folhas de Petiveria alliaceae L.(Phytolacaceae). Revista da Universidade Vale do Rio Verde, v.14, n.1, p.448-457, 2016. doi: 10.5892/ruvrd.v14i1.2525

BEHBAHANI B. A. et al. Effect of aqueous and ethanolic extract of Eucalyptus camaldulensis L. on food infection and intoxication microorganisms “in vitro”. Journal of Paramedical Sciences, v.4, n.3, p.89-99, 2013. doi: 10.22037/jps.v4i3.4666

BRAND-WILLIAMS, W.; CULIVER M.E.; & BERSET, C. Use of a free radical method to evaluate antioxidant activity. LWT – Food Science and Tecnology, v.28, n.1, p.25-30, 1995. doi: 10.1016/S0023-6438(95)80008-5

BRUZADELLI, R. F. D. et al. Composição química, atividade antioxidante e qualidade microbiológica do extrato aquoso de Mentha piperita, de acordo com o tempo de decocção. Brazilian Journal of Development, v. 6, n. 4, p. 17165-17171, 2020. doi: 10.34117/bjdv6n4-039

CARDOSO R. C. et al. Potencial antimicrobiano do óleo da folha de Eucalyptus urograndis frente Stafilococus aureus. Id on Line Revista Multidisciplinar e de Psicologia, v.13, n.43, p.989-1002, 2019. doi: 10.14295/idonline.v13i43.1573

DE VECHI A.; & JÚNIOR C. A. O. M. Aspectos positivos e negativos da cultura do Eucalipto e os efeitos ambienteis do seu cultivo. Revista Valore, v.3, n.1, p.495-507, 2018. doi: 10.22408/reva312018101495-507

ELANSARY H. O. et al. In vitro antibacterial, antifungal and antioxidant activities of Eucalyptus spp. leaf extracts related to phenolic composition. Natural product research, v.31, n.24, p.2927-2930, 2017. doi: 10.1080/14786419.2017.1303698

FERNÁNDEZ-AGULLÓ, A.; FREIRE, M. S.; & GONZÁLEZ-ÁLVAREZ, J. Effect of the extraction technique on the recovery of bioactive compounds from eucalyptus (Eucalyptus globulus) wood industrial wastes. Industrial Crops and Products, v.64, p.105-113, 2015. doi: 10.1016/j.indcrop.2014.11.031

GARRIDO, G.; ORTIZ, M.; & POZO, P. Fenoles y flavonoides totales y actividad antioxidante de extractos de hojas de Lampaya medicinalis F. Phil. Journal of Pharmacy & Pharmacognosy Research, v.1, n.1, p.30-38, 2013. ISSN: 0719-4250.

GHAREKHANI, M.; GHORBANI, M.; & RASOULNEJAD, N. Microwave-assisted extraction of phenolic and flavonoid compounds from Eucalyptus camaldulensis Dehn leaves as compared with ultrasound-assisted extraction. Latin American applied research, v.42, n.3, p.305-310, 2012. ISSN 0327-0793.

GUERRA, A. P.; DOS SANTOS GARCIA, V. A.; & DA SILVA, C. Otimização da extração de compostos fenólicos da casca de manga (Tommy atkins) utilizando processo assistido por ultrassom. E-xacta, v.9, n.1, p.103-110, 2016. doi: 10.18674/exacta.v9i1.1783

HERRERO M. et al. Compressed fluids for the extraction of bioactive compounds. TrAC Trends in Analytical Chemistry, v.43, p.67-83, 2013. doi: 10.1016/j.trac.2012.12.008

LI W. et al. In vitro and in vivo antioxidant activity of eucalyptus leaf polyphenols extract and its effect on chicken meat quality and cecum microbiota. Food Research International, v.136, p.109302, 2020. doi: 10.1016/j.foodres.2020.109302

LIMAM H. et al. Phenolic composition and antioxidant activities of thirteen Eucalyptus species cultivated in north east of Tunisia. Plant Biosystems-An International Journal Dealing with all Aspects of Plant Biology, p.1-18, 2020. doi: 10.1080/11263504.2020.1762791

LUÍS Â. et al. Stumps of Eucalyptus globulus as a source of antioxidant and antimicrobial polyphenols. Molecules, v.19, n.10, p.16428-16446, 2014. doi: 10.3390/molecules191016428

MIGLIATO K.F. et al. Planejamento experimental na otimização da extração dos frutos de Syzygium cumini (L.) Skeels. Química Nova, v.34, n.4, p.695-699, 2011. doi: 10.1590/S0100-40422011000400024

MUHAMMED, D.; DADA, E. O.; & ALO, A. A. Antibacterial property of ethanolic leaf extract of Eucalyptus citriodora hook on clinical and typed isolates of Escherichia coli. South Asian Journal of Research in Microbiology, v.2, n.1, p.1-8, 2018. doi: 10.9734/sajrm/2018/v2i129234

PAES J. et al. Extraction of phenolic compounds and anthocyanins from blueberry (Vaccinium myrtillus L.) residues using supercritical CO2 and pressurized liquids. Journal of Supercritical Fluids, v.95, p.8-16, 2014. doi: 10.1016/j.supflu.2014.07.025

PEREIRA V. et al. Antibacterial activity and synergistic effects between Eucalyptus globulus leaf residues (essential oils and extracts) and antibiotics against several isolates of respiratory tract infections (Pseudomonas aeruginosa). Industrial Crops and Products, v.52, p.1-7, 2014. doi: 10.1016/j.indcrop.2013.09.032

ROSSA U. B. et al. Influência da luminosidade e fertilizantes nos teores de metilxantinas e compostos fenólicos em folhas de erva-mate. Ciência Florestal, v.27, n.4, p.1365-1374, 2017. doi: 10.5902/1980509830217

RUDKE A. R. et al. Optimization of green PLE method applied for the recovery of antioxidant compounds from buriti (Mauritia flexuosa L.) Shell. Food Chemistry, v.298, p.125061, 2019. doi: 10.1016/j.foodchem.2019.125061

SAMPAIO A. A. et al. Bioatividade dos extratos aquosos de folhas de pimenta malagueta e eucalipto sobre Zabrotes subfasciatus em grãos de fava. Revista Verde de Agroecologia e Desenvolvimento Sustentável, v.12, n.2, p.198-203, 2017. doi: 10.18378/rvads.v12i2.4695

SINGLETON, V. L.; ORTHOFER, R.; & LAMUELA-RAVENTOS, R. M. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteau reagente. Methods of Enzymology, v.299, p.152-178, 1999. doi: 10.1016/S0076-6879(99)99017-1

SOARES, A. A. et al. Comparison of total phenolic content and antioxidant activity of different extracts of Agaricus blazei Murril. Brazilian Journal of Development, v. 6, n. 3, p. 13561-13573, 2020. doi: 10.34117/bjdv6n3-281

SOUZA L. P. et al. Composição química e toxicidade do óleo essencial de eucalipto sobre o ácaro-rajado. Comunicata Scientiae, v.7, n.4, p.486-493, 2016. doi: 10.14295/cs.v7i4.1502

SYUKRI, D. M.; NWABOR, O.; & ERGANTARA, R. I. Preliminary phytochemical, antioxidants, and antibacterial properties of eucalyptus aqueous leaf extract against Streptococcus pyogenes. In IOP Conference Series: Earth and Environmental Science, IOP Publishing, v.305, n.1, p.012067, 2019. doi: 10.1088/1755-1315/305/1/012067

TAVANTI R. F. R. et al. Eficiência da adubação boratada no desenvolvimento de mudas de eucalipto. Revista de Ciências Agrárias Amazonian Journal of Agricultural and Environmental Sciences, v.61, 2018. doi: 10.22491/rca.2018.2862

ZHAO S.; & ZHANG, D. Supercritical CO2 extraction of Eucalyptus leaves oil and comparison with Soxhlet extraction and hydro-distillation methods. Separation and Purification Technology, v.133, p.443-451, 2014. doi: 10.1016/j.seppur.2014.07.018




DOI: https://doi.org/10.34117/bjdv6n10-032

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