Warifteine therapeutic treatment reduced leukocyte recruitment and anxiety-like response in ovalbumin-induced allergic pulmonary inflammation / Tratamento terapêutico com warifteína reduz recrutamento de leucócitos e resposta semelhante á ansiedade na inflamação pulmonar alérgica induzida por ovalbumina

Hianka Greice Lopes de Sousa, Laércia Karla Diega Paiva Ferreira, Humberto de Carvalho Aragão Neto, Atalia Ferreira de Lima Flôr, José Maria Barbosa- Filho, José Luiz de Brito Alves, Valdir de Andrade Braga, Reinaldo Nóbrega de Almeida, Marcia Regina Piuvezam, Cláudio Roberto Bezerra dos Santos

Abstract


Pulmonary inflammation plays a fundamental role in the pathophysiology of allergic asthma, which is characterized by lower airway obstruction, bronchial hyperresponsiveness, tissue remodeling, recruitment of inflammatory cells, with a predominance of eosinophils, in addition to behavioral disorders such as anxiety. The aim of this study was to evaluate the therapeutic effect of the alkaloid warifteine, from the medicinal plant Cissampelos sympodialis, on anxiety-like behavior, respiratory frequency and leukocyte recruitment in an experimental model of allergic pulmonary inflammation. Swiss female mice were sensitized and challenged with ovalbumin (OVA) throughout the experimental protocol. The animals were treated orally with warifteine (2 mg / kg), subcutaneously with dexamethasone (2 mg / kg) or intraperitoneally with diazepam (1 mg / kg), 1 h after the OVA-challenges. On the last day of the antigenic challenge, the mice were tested for behavior using the Elevated Plus Maze (EPM) and for respiratory rate using full body plethysmography. The following day, the mice were euthanized to collect the bronchoalveolar lavage fluid (BALF) and leukocyte count. The data obtained showed that OVA-sensitization induced a behavior similar to anxiety in mice since the EPM test showed that the OVA group increased the number of entries and the time spent in the closed arms (CA) of the apparatus and reduced these parameters in the open arms (OA) compared to the Salina group. Warifteine treatment reversed both parameters analyzed, increasing the time spent (p <0.0001) and number of entries (p <0.01) in OA, decreasing the time spent (p <0.01) and number of entries (p <0.0001) in the CA, similarly to dexamethasone and diazepam standard drugs. Warifteine also reduced the respiratory rate (p <0.01) compared to the OVA group. The behavioral and breathing changes of the tested animals showed a relationship with the increase in the total and differential inflammatory leukocyte number in the OVA group compared to the Saline group. Therapeutic treatment with warifteine decreased the inflammatory process, reducing the number of total cells (p <0.0001) dependent of eosinophils and neutrophils numbers (p <0.001), as well as the percentage of eosinophils (p <0.0001). These data show that therapeutic treatment with warifteine is able to inhibit anxiety-like behavior and respiratory rate, due to a mechanism related to the inhibition of eosinophilic migration in an experimental model of allergic pulmonary inflammation.


Keywords


Alkaloid, Airway disease, Psychological disorder.

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DOI: https://doi.org/10.34117/bjdv6n9-124

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