Selection and isolation of fungi strains and the use of banana peel a suport for lipases production / Isolamento e seleção de linhagens fúngicas e o uso da casca de banana como suporte para produção de lipases

Authors

  • Mariane Daniella da Silva Brazilian Journals Publicações de Periódicos, São José dos Pinhais, Paraná
  • Fernanda Maria Pagane Guereschi Ernandes
  • Crispin Humberto Garcia Cruz

DOI:

https://doi.org/10.34117/bjdv6n7-685

Keywords:

microbial enzymes, substrates, semi-solid fermentation.

Abstract

Lipases are important enzymes in biotechnological processes, mainly due to their ability to catalyze a wide range of reactions of interest to the food and pharmaceutical industry, including anti-cholesterol, anti-inflammatory and thrombolytic agents, in the chemical industry, among others. These enzymes can modify the properties of lipids either by changing the position of the fatty acid chain in the molecule, or by exchanging one or more acids for different ones. Lipases are obtained from animal, vegetable or microbial cells, however, those of microbial origin are currently the most used industrially. This work aimed to isolate and select fungi that produce lipolytic enzymes, as well as the study of the production of these enzymes by semi-solid fermentation using banana peel and using residues from the agribusiness, wheat bran and bagasse of sugar cane.  After the selection process, using baits soaked in soy oil, olive oil and coconut milk, the isolates with the highest enzyme index were used to study the production of lipase using residues in fermentation. Among the isolates selected as lipase producers, maximum production was achieved by the MO 56 strain from baits soaked in soybean oil, when 40% of wheat bran was used as a substrate and banana peel as a support, resulting in 1.46 µmol.g-1 of enzymatic activity. The choice of banana peel as a support proved to be adequate and its granulometry provided an appropriate aeration for the supports, preventing their compaction, in addition to maintaining their homogeneous humidity without showing exudation throughout the fermentation process.

References

Adrio JL, Demain AL. Microbial Enzymes: Tools for Biotechnological Processes. Biomolec. 2014 Feb;4(1):117-39, doi: 10.3390/biom4010117

Bon EPS, Ferrara MA, Corvo ML, Vermelho AB, Paiva CLA, Alencastro RB, Coelho RRR. Enzimas em Biotecnologia: Produção, Aplicações e Mercado. Interciência 1:506, 2008.

Borgström B, Brockman H. Lipases. New York: Ed. Elsevier, 1984.

Carvalho NB, Lima AS, Soares CMF. Uso de sílicas modificadas para imobilização de lipases. Quim. Nova. 2015 Mar;38(3):399-409, doi: https://doi.org/10.5935/0100-4042.20140304

Carvalho PO, Calafatti SAp, Marassi M, Silva DM, Contesini FJ, Bizaco R. Potencial de biocatálise enantiosseletiva de lipases microbianas. Química Nova, 2005 Aug;28(4):614-621, doi: https://doi.org/10.1590/S0100-40422005000400012

Castilho LR, Polato CMS, Baruque EA, Sant?Anna GGJr, Freire DMG. Economic analysis of lipase production by Penicillium restrictum in solid-state and submerged fermentation. Biochem. Eng. J., 2000 Fev;4(3):239-247, doi: 10.1016 / S1369-703X (99) 00052-2

Coimbra MC, Pinto TOP, D´Arcadia Cruz V, Oliveira Neto P. Isolation and selection of lipolytic enzymes-producing fungi. Rev. Inst Adolfo Lutz. 2012 Maio71(1):50

Da Silva, MOMB, Bispo, DF, Pagano, RL, Silva, AS, Santos EJ, Silva CF. Isolamento de microrganismos e estudo da produção de lípase utilizando resíduos agroindustriais. Scientia Plena, 2016 Maio;12(5):54214, doi: http://dx.doi.org/10.14808/sci.plena.2016.054214

Diaz JCM., Rodriguez JA, Roussos S, Cordova J, Abousalham A, Carriere F, Baratti J. Lipase from the thermotolerant fungus Rhizopus homothallicus is more thermostable when produced using solid state fermentation than liquid fermentation procedures. Enzyme and Microbial Technology, 2006 Set;39(5):1042-1050, doi: https://doi.org/10.1016/j.enzmictec.2006.02.005

Gandra KM, Del Biachi M, Godoy VP, Queiroz EPC, Steel CJ. Application of lipase and monoglyceride in fiber enriched pan bread. Food Science and Technology. 2008 Mar;28(1):196, doi: https://doi.org/10.1590/S0101-20612008000100027

Gupta S, Bhattacharya A, Murthy CN. Tune to immobilize lipases on polymer membranes: Techniques, factors and prospects – Review. Biocatalysis and Agricultural Biotechnology, 2013 Jul;2(3):171-190, doi: https://doi.org/10.1016/j.bcab.2013.04.006

Hankin L, Anagnostakis SG. The use of solid media for detection of enzyme production by fungi. Mycology, 1975 Jun;67(3):597-607, doi: 10.2307 / 3758395

Karthikeyan A, Sivakumar N. Citric acid production by Koji fermentation using banana peel as a novel substrate. Bioresource Technology, 2010 v. 101. p. 5552–5556. 2010.

Lin ES, Wang CC, Sung SC. Cultivating conditions influence lipase production by the edible Basidiomycete Antrodia cinnamomea in submerged culture. Enzyme and Microbial Technology, 2006 Jun;39(1):98-102, doi: https://doi.org/10.1016/j.enzmictec.2005.10.002

Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin Phenol Reagent. J Biot Chem, 1951 Nov;193(1):265-275

Mahadik ND et al. Production of acidic lipase by Aspergillus niger in solid state fermentation. Process Biochemistry, 2002 Dec;38(5):715-721, doi: 10.1016 / S0032-9592 (02) 00194-2

Marotti BS, Cortez DV, Gonçalvez DB, Castro HF. Seleção de espécies do gênero Penicillium produtoras de lipase ligada ao micélio para Aplicação em hidrólise de óleos vegetais. Quim Nova. 2017 40(4):435, doi: https://doi.org/10.21577/0100-4042.20170033

Moura IAA, et al. Biomassa proveniente da casca da banana Musa sapientum: pre-tratamento e hidrólise ácida para análise da viabilidade na produção de bioetanol. Brazilian Journal of Development. 2020 6(1):1975-1987, doi:10.34117/bjdv6n1-140

Oliveira AN. et al. Extracellular hydrolytic enzymes in indigenous strains of rhizobia in Central Amazonia, Amazonas, Brazil. Food Science and Technology, 2006 Out;26(4):853-860, doi: 10.1590/S0101-20612006000400022

Rodriguez-Zuniga UF et al. Produção de celulases por Aspergillus niger por fermentação em estado sólido. Pesq. agropec. Bras. 2011 Jul;46(8):912-919, doi: https://doi.org/10.1590/S0100-204X2011000800018

Roveda M, Hemkemeier M, Colla LM. Avaliação da produção de lipases por diferentes cepas de microrganismos isolados em efluentes de laticínios por fermentação submersa. Ciência e Tecnologia de alimentos, 2010 30(1):126-131, doi: https://doi.org/10.1590/S0101-20612010000100019

Singh AK, Mukhopadhyay M. Overview of Fungal. Lipase: A Review. Appl Biochem Biotechnol. 2012 Mar;166(2):486-520, doi: https://doi.org/10.1002/jls.20239

Sirisha VL, Ankita Jain A, Jain A. Enzyme Immobilization: An Overview on Methods, Support Material, and Applications of Immobilized Enzymes. Advances in Food and Nutrition Research, 2016 Set;79:179-211, doi: 10.1016 / bs.afnr.2016.07.004

Torres, M.; Dolcet, M.M.; Sala, N.; Canela, R. Endophytic fungi associated with mediterranean plants as a source of mycelium-bound lipases. J. Agric. Food Chem. 2003 Maio;51(11):3328-3333, doi: 10.1021 / jf025786u

Treichel H, Sbardelotto M, Venturin B, Dall Agnol A, Mulinari J, Golunski SM, Baldoni DB, Bevilacqua CB, Jacques RJS, Vargas GDLP, Mossi AJ. Lipase Production from a newly Isolated Aspergillus Niger by Solid State Fermentation using Canola Cake as Substrate. Curr Biotechnol. 2016 Feb;5:1-7, doi: 10.2174/2211550105666151124193225t

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Published

2020-07-27

How to Cite

Silva, M. D. da, Ernandes, F. M. P. G., & Cruz, C. H. G. (2020). Selection and isolation of fungi strains and the use of banana peel a suport for lipases production / Isolamento e seleção de linhagens fúngicas e o uso da casca de banana como suporte para produção de lipases. Brazilian Journal of Development, 6(7), 51310–51320. https://doi.org/10.34117/bjdv6n7-685

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Original Papers