Comparison between four colpocytological examination collection techniques in cats / Comparação entre quatro técnicas de colheita para exame colpocitológico em gatas

Myrian Megumy Tsunokawa Hidalgo, Jamile Haddad Neta, Ana Beatriz Marques de Almeida, Luiz Guilherme Corsi Trautwein, Maria Isabel Mello Martins


Colpocytology has practical applications in the feline species, such as monitoring of the estrous cycle phases, aiding in the diagnosis of hormonal disorders and diseases of the female genital tract. The aim was to compare the most efficient method for performing vaginal cytology in queens. Vaginal material was collected from 12 female cats, healthy, aged between one and eight years of age, with a mean weight of 3.5 kg. The queens were divided into four groups, each with a different tool for collection of vaginal cells. GIB: nylon bristles (Interdental fine brush- GUM®); GGB: gynecological brush for humans (endobrush- INLAB®); GPP: Pasteur pipette (graduated-3mL); and GS: sterile cotton swab (ABSORVE®). Cells were deposited on a microscope slide to obtain the vaginal smears and stained with rapid panoptic dye. The smear cell-based classification was performed subjectively, establishing scores from 0 to 4 (0 absence of cells, 1 very few cells, 2 few cells, 3 moderate cell numbers, 4 high cell numbers). The materials obtained were well preserved and in adequate quantity for cytological evaluation. A descriptive statistical analysis was performed to compare the collection methods. It was observed that the average swab cellularity score collected with the gynecological brush (GGB = 3.13) was higher compared to the other groups: interdental brush (GIB = 2.2), Pasteur pipette (GPP= 2.13), and swab (GS = 0.8). Although the swab is the most commonly used method in the colpocytological examination in cats, the interdental brush proved to be the most efficient method to collect samples, providing great cellular recovery and a low degree of discomfort.


vaginal cytology, cervical brush, interdental brush, felines, Pasteur pipette, swab.


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