Identification and genotyping of white spot syndrome virus (WSSV) isolated from Litopenaeus vannamei in the state Rio Grande do Norte / Identificação e genotipagem do vírus da síndrome da mancha branca (WSSV) isolado de Litopenaeus vannamei no estado do Rio Grande Do Norte

Emanuelly Cristina Rodrigues Peixoto, Rebeca Jéssica Falcão Câmara, Germana Guimarães Rebouças, Bruno Vinicios Silva de Araújo, Sidnei Miyoshi Sakamoto

Abstract


The aim of our investigation was to detect WSSV in Litopenaues vannamei from farms in Rio Grande do Norte (RN) state, as well as perform genotyping of the isolates. A total of five farms were surveyed, from which 10 samples were collected in each one. We selected two ponds in each of the farms; these were chosen because of clinical suspicion of WSSV. Genomic DNA was extracted to detect the virus by polymerase chain reaction (PCR). Subsequently, we performed, through PCR, genotyping of the positive DNA samples analyzing a variable number of tandem repeats - VNTR in three molecular markers (ORFs 75, 94 and 125). The results revealed the presence of the virus in only one of the 5 analyzed farms. The loci ORF 75 and ORF 94 were identified in isolates from the positive samples and no amplification failure for ORF 125. The number of repeating units (RUs) was calculated, and found to 9 repetitions ORF 75 and 10 RUs to ORF 94, a pattern that had not been described, stressing the need for more strain typing for screening and surveillance of the disease.

Keywords


ORF 75, ORF 94, ORF 125, VNTR, WSSV.

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References


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DOI: https://doi.org/10.34117/bjdv6n7-236

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