Comparative analysis of salt-induced changes in the leaves proteome of two contrasting Jatropha curcas genotypes / Análise comparativa no proteoma, induzido por salinidade, em folhas de dois genótipos contrastantes de Jatropha curcas

Natália Corte-Real, Melquisedec S. Oliveira, Alfredo Jarma-Orozco, Denise Fernandes, Marcos A. dos Santos, Laurício Endres, Tercílio Calsa Junior, Marcelo F. Pompelli


The salt stress is one of the major abiotic stress factors limiting the productivity of many agricultural plant species. However, as a sessile organism, plant might adjust their metabolism reprograming many different complex pathway aiming tolerate such different stresses by activating genes and transcriptional factors. Here we investigated the protein differential salt tolerance in two contrasting Jatropha curcas genotypes with a special emphasis on the proteomic changes in the leaves, contributing to the identification of candidate proteins for molecular markers in response to salinity tolerance. 6-months J. curcas plants were kept under 750 mM NaCl salt concentration. After 40 hours of stress, leaves were harvested and protein profile analyzed. Total proteins were extracted, purified and quantified. As results, we identify 110 salinity-responsive differently accumulated proteins in J. curcas, presumably associated with metabolic processes of ADP, ribonucleotides, carbohydrate and pyruvate derivatives, as well as ATP biosynthesis and response to metal ions as the main biological processes associated to tolerant-like J. curcas genotype. The comparative proteome revealed that 110 proteins were salt-responsive in both genotype, while 69 and 41 protein were salt responsive in the CNPAE183 and in CNPAE218, respectively. The tolerant-like genotype presented proteins from different pathways mainly for the salinity response, including proteins involved in signaling, antioxidant metabolism, as well as key enzymes from other metabolic pathways of energy production, such as photosynthesis and glycolysis, suggesting the maintenance of their function growth and development. Our results gave deeper insights into plasticity of salt tolerance responses of J.curcas cultivated under field-condition.


purging nut, NaCl, salt stress, peptide, mass spectrometry, 2D electrophoresis


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