Antagonistic effect of Trichoderma isolates and its metabolites against Fusarium solani and F. oxysporum in chickpea / Efeito antagonista de isolados de Trichoderma e seus metabólitos contra Fusarium solani e F. oxysporum em grão-de-bico

Daiana Maria Queiroz Azevedo, Fernando da Silva Rocha, Maria de Fátima Gonçalves Fernandes, Cândido Alves da Costa, Maria de Fátima Silva Muniz, Patrícia Döerl Barroso, Fernanda Letycia Amaral, Dandara Maria Clara do Rosário Barbosa

Abstract


The aim of this work was to study and select Trichoderma sp. strains with biocontrol potential against Fusarium solani and F. oxysporum, the causal agents of root rot and wilt in chickpea. Antagonism against pathogenic isolates of F. solani and F. oxysporum of twenty-one isolates of Trichoderma sp. obtained from the rhizosphere of chickpea plants were evaluated in vitro through competition in dual culture tests as well as the production of volatile and non-volatile organic compounds exhibiting fungicidal and/or fungistatic activity. In the in vivo experiment, four isolates of Trichoderma sp. were selected and their antagonism was evaluated separately and combined with a commercial product based on T. asperellum under greenhouse conditions. Trichoderma sp. isolates were efficient competitors and produced metabolites capable of inhibiting mycelial growth of both species of Fusarium. Our results show the great versatility of the mechanisms of action from Trichoderma isolates, mainly associated with the production of volatile organic compounds. Despite the antagonistic effect of Trichoderma isolates observed in vitro, these isolates did not control Fusarium neither promote chickpea growth in in vivo conditions.

 


Keywords


Cicer arietinum, biological control, fusariosis, secondary metabolites

Full Text:

PDF

References


Aguiar, A. R.; Machado, D. F. M; Paranhos, J. T.; Silva, A. C. F. Seleção de isolados de Trichoderma spp. na promoção de crescimento de mudas do feijoeiro cv. Carioca e controle de Sclerotinia sclerotiorum. Ciência e Natura, v.34, p.47-58, 2012.

Almeida, F. B.; Cerqueira, F. M.; Silva, R. N.; Ulhoa, C. J.; Lima, A. L. Mycoparasitism studies of Trichoderma harzianums strains against Rhi¬zoctonia solani: evaluation of coiling and hydrolytic enzyme production. Biotechnology Letters, v. 29, p.1189-1193, 2007.

Azevedo, D. M. Q.; Rocha, F. S.; Costa, C. A.; Pfenning, L. H.; Costa, S. S.; Melo, M. P., Silva, J. G.; Fernandes, M. F. G. Etiology of root rot and wilt disease of chickpea in Brazil. Tropical Plant Pathology, v.42, p.273-283, 2017.

Batson-Girona, I.; Vinale, F.; Ruano-Rosa, D.; Lorito, M.; López-Herrera, C. J. Effect of metabolites from different Trichoderma strains on the growth of Rosellinia necatrix, the causal agent of avocado white root rot. European Journal of Plant Patholology, v.140, p.385-397, 2014.

Bell, D. K.; Wells. H. D.; Markham, C. R. In vitro antagonism of Trichoderma species against six fungal plant pathogens. Phytopathology, v.72, p.379-382, 1982.

Benítez, T.; Rincón, A. M.; Limón, M. C. E; Condón, A. C. Biocontrol mechanisms of Trichoderma strains. International Microbiology, v.7, p.249-260, 2004.

Bettiol, W.; Morandi, M. A. B.; Pinto, Z. V.; Júnior, T. J. P.; Corrêa, E. B.; Moura, A. B.; Lucon, C. M. M.; Costa, J. C. B; Bezerra, J. L. (2012). Produtos comerciais à base de agentes de biocontrole de doenças de plantas. Embrapa Meio Ambiente, Jaguariúna-SP, 2012.155 p. (Documentos / Embrapa Meio Ambiente; 88).

Castellani, A. Viability of some pathogenic fungi in distilled water. Journal of Tropical Medicine and Hygiene, v. 42, p.225-226, 1939.

Contreras-Cornejo, H. A.; Macías-Rodríguez, L.; Cortés-Penagos, C.; López-Bucio, J. Trichoderma virens, a plant beneficial fungus, enhances biomass production and promotes lateral root growth through an auxin-dependent mechanism in Arabidopsis. Plant Physiology, v.149, p.1579-1592, 2009.

Daniel, H. C. F.; Wilfredo, F. F.; Francisco, C. R.; Gabriel, G. M.; Epifanio, C. A. Antibiosis in vitro of Trichoderma strains metabolic extract on mycelial growth and reproductive capacity of Fusarium oxysporum isolated from pepper plants (Capsicum annuum L.). British Biotechnology Journal, v.4, p.387-399, 2014.

Dennis, C.; Webster J. Antagonistic properties of species groups of Trichoderma: I. Production of nonvolatile antibiotics. Transactions of the British Mycological Society, v.57, p.25-39, 1971a.

Dennis, C.; Webster J. Antagonistic properties of species groups of Trichoderma: II. Production of volatile antibiotics. Transactions of the British Mycological Society, v.57, p.41-48, 1971b.

Dennis, C.; Webster J. Antagonistic properties of species-groups of Trichoderma: III. Hyphal interactions. Transactions of the British Mycological Society, v.57, p.363-369, 1971c.

Dubey, S. C.; Suresh, M.; Singh, B. Evaluation of Trichoderma species against Fusarium oxysporum f. sp. ciceris for integrated management of chickpea wilt. Biological Control, v. 40, 118-127, 2007.

Ethur, L. Z.; Blume, E.; Muniz, M.; Silva, A. C. F.; Stefanelo, D. R.; Rocha, E. K. Fungos antagonistas a Sclerotinia sclerotiorum em pepineiro cultivado em estufa. Fitopatologia Brasileira, v.30, p. 127-133, 2005.

Eziashi, E. I.; Uma, N. U.; Adekunle, A. A.; Airede, C. E. Effect of metabolites produced by Trichoderma species against Ceratocystis paradoxa in culture medium. African Journal of Biotechnology, v.5, p.703-706, 2006.

Harman, G. E.; Howell, C. R.; Viterbo, A.; Chet, I.; Lorito, M. Trichoderma species-opportunistic, avirulent plant symbionts. Nature Reviews Microbiology, v.2, p.43-56. 2004.

Isaias, C. O.; Martins, I.; Silva, J. B. T.; Silva, J. P.; Mello, S. C. M. Ação antagônica e de metabólitos bioativos de Trichoderma spp. contra os patógenos Sclerotium rolfsii e Verticillium dahliae. Summa Phytopathologica, v. 40, p.34-41, 2014.

Jiménez-Gasco, M. M.; Navas-Cortes, J.; Jiménez-Diaz, R. M. Evolucion de Fusarium oxysporum f. sp. ciceris, el agente de la Fusariosis vascular del garbanzo, en razas patogenicas y patotipos. Boletin de Sanidad Vegetal Plagas, v.31, p.59-69, 2005.

Jyotsna, A. S.; Singh, R. P.; Srivastava, A. K.; Saxena, A. K.; Arora, D. K. Growth promotion and charcoal rot management in chickpea by Trichoderma harzianum. Journal of Plant Protection Research, v.48, p.81-92, 2008.

Keszler, A.; Forgács, E.; Kótai, L. Separation and identification of volatile components in the fermentation broth of Trichoderma atroviride by solid-phase extraction and gas chromatography–mass spectrometry. Journal of Chromatographic Science, v.38, p.421–424, 2000.

Louzada, G. A. S.; Carvalho, D. D. C.; Mello, S. C. M.; Lobo Júnior, M.; Martins, I.; Braúna, L. M. Antagonist potential of Trichoderma spp. from distinct agricultural ecosystems against Sclerotinia sclerotiorum and Fusarium solani. Biota Neotropica, v.9, p.145-149, 2009.

Machado, D. F. M.; Parzianello, F. R.; Silva, A. C. F. D.; Antoniolli, Z. I. Trichoderma no Brasil: o fungo e o bioagente. Revista de Ciências Agrárias, v.35, p.274-288, 2012.

Mulatu, A.; Megersa, N.; Alemu, T. Characterization of fungal extracts from Trichoderma isolates: their effects against coffee wilt pathogen (Gibberella xylarioides). Ethiopian Journal of Science, v.36, p.81-92, 2013.

Nene, Y. L.; Reddy, M. V.; Haware, M. P.; Ghanekar, A. M., Amin, K. S., Pande, S.; Sharma, M. Field diagnosis of chickpea diseases and their control. Patancheru: International Crops Research Institute for the Semi-Arid Tropics, 2012. 60p. (Information Bulletin No. 28).

Ram, H. M.; Kendurkar, S. V. Modes of parasitism between the necrotrophic fungus Botrytis cinerea and Trichoderma spp. Journal of Biology, Agriculture and Healthcare, v.4, 2014. Available at: https://www.iiste.org/Journals/inndex.php/JBAH/article/view/17412/20826.

Siddiquee, S.; Cheong, B. E.; Taslima, K.; Kausar, H.; Hasan, M. M. Separation and identification of volatile compounds from liquid cultures of Trichoderma harzianum by GC-MS using three different capillary columns. Journal of Chromatographic Science, v.50, p.58-367, 2012.

Singh, G.; Chen, W.; Rubiales, D.; Moore, K.; Sharma, Y. R.; Gan, Y. Diseases and their management. In: Yadav, S. S.; Redden, R. J.; Chen, W.; Sharma, B. (Eds). Chickpea breeding and management. India: CAB International, 2007. p. 497-519.

Souza Pedro, E. A.; Harakava, R.; Lucon, C. M. M.; Guzzo, S. D. Promoção do crescimento do feijoeiro e controle da antracnose por Trichoderma spp. Pesquisa Agropecuária Brasileira, v. 47, p.1589-1595, 2012.

Vinale, F.; Marra, R.; Scala, F.; Ghisalberti, E. L.; Lorito, M.; Sivasithamparam, K. Major secondary metabolites produced by two commercial Trichoderma strains active against different phytopathogens. Letters in Applied Microbiology, v.43, p.143-148, 2006.

Vinale, F.; Sivasithamparam, K.; Ghisalberti, E. L.; Marra, R.; Woo, S. L.; Lorito, M. Trichoderma-plant-pathogen interactions. Soil Biology and Biochemistry, v.40, p.1-10 2008.

Zafari, D.; Koushki, M. M.; Bazgir, E. Biocontrol evaluation of wheat take-all disease by Trichoderma screened isolates. African Journal of Biotechnology, v.7, p.3653-3659, 2008.

Zhang, F.; Yang, X.; Ran, W.; Shen, Q. Fusarium oxysporum induces the production of proteins and volatile organic compounds by Trichoderma harzianum T-E5. FEMS Microbiology Letters, v.359, p.116-123, 2014.




DOI: https://doi.org/10.34117/bjdv6n6-251

Refbacks

  • There are currently no refbacks.