Molecular detection of Leishmania infantum, Babesia vogeli, and Rangelia vitalii in dogs from the Embu-Guaçú municipality around the Guarapiranga Reservoir, São Paulo / Detecção molecular de Leishmania infantum, Babesia vogeli e Rangelia vitalii em cães do municipio de Embú-Guaçú no entorno da represa Guarapiranga, São Paulo

Giovanna Stefani Nosberto Castelli, Roberta Carvalho de Freitas e Azevedo, Ryan Emiliano da Silva, Jaciara de Oliveira Jorge Costa, Renata Tonhosolo, Jonas Moraes Filho, Arlei Marcili

Abstract


Vector-borne diseases are of great importance worldwide in human and veterinary medicine, as several parasites can cause disease in multiple different hosts. In this work, we investigated the prevalence of some vector-borne parasitic diseases in dogs in Embu-Guaçú, São Paulo, Brazil. Molecular diagnosis was performed by PCR using oligonucleotide markers specific for protozoans with significance in both animal and human health, such as Leishmania infantum, Rangelia vitalii, and Babesia vogeli. Molecular diagnostic results showed a high occurrence (16.25%, 13/80) of Babesia vogeli, but negative results for Leishmania infantum and Rangelia vitalii. Further studies should be conducted with a larger number of dogs, and vector control and educational measures should be implemented within the population to minimize the impact of vector-borne diseases on animal health.


Keywords


Sandfly; Tick; Zoonosis; Atlantic Rainforest, Vectors

Full Text:

PDF

References


ARAUJO AC, SILVEIRA JAG, AZEVEDO SS, NIERI-BASTOS FA, RIBEIRO MFB, LABRUNA MB, HORTA MC. Babesia canis vogeli infection in dogs and ticks in the semiarid region of Pernambuco, Brazil. Pesq Vet Bras, v. 35, p. 346-461, 2015.

AZEVEDO TS, LORENZ C, CHIARAVALLOTI-NETO F. Risk mapping of visceral leishmaniasis in Brazil. Rev Soc Bras Med Trop, v. 52, 2019.

BANETH G. Antiprotozoal treatment of canine babesiosis. Vet Parasitol, v. 254, p. 58-63, 2018.

BRAGA JFV, SILVA SMMS. Babesiose canina: uma visão geral da doença. Rev Ciênc Agrovet, v. 12, p 204-213, 2013.

CAMARGO-NEVES V. A Leishmaniose Visceral Americana no Estado de São Paulo: situação atual. São Paulo: Secretaria de Estado da Saúde de São Paulo. Bepa, v. 4, p. 12-14, 2008.

CARDIM MFM, RODAS LAC, DIBO MR, GUIRADO MM, OLIVEIRA AM, CHIARAVALLOTI-NETO F. Introdução e expansão da Leishmaniose visceral americana em humanos no estado de São Paulo. Rev Saúde Pública, v. 47, p. 691-700, 2013.

CARDIM MFM, GUIRADO MM, DIBO MR, CHIARAVALLOTI-NETO F. Visceral leishmaniasis in the state of Sao Paulo, Brazil: spatial and space-time analysis. Rev Saúde Pública, v. 50, 2016.

CARRET C, WALAS F, CARCY B, GRANDE N, PRÉCIGOUT E, MOUBRI K, SCHETTERS TP, GORENFLOTET A. Babesia canis canis, Babesia canis vogeli, Babesia canis rossi: Differentiation of the Three Subspecies by a Restriction Fragment Length Polymorphism Analysis on Amplified Small Subunit Ribosomal RNA Genes. J Eukaryot Microbiol, v. 46, p. 298-303, 1999.

COSTA-JUNIOR LM, ZAHLER-RINDER M, RIBEIRO MFB, REMBECK K, RABELO EML, PFISTER K, PASSOS LMF. Use of a Real Time PCR for detecting subspecies of Babesia canis. Vet Parasitol, v. 188, p. 160-163, 2012.

DA SILVA AS, FRANÇA RT, COSTA MM, PAIM CB, PAIM FC, DORNELLES GL, Soares JF, Labruna MB, Mazzanti CM, Monteiro SG, Lopes STA. Experimental infection with Rangelia vitalii in dogs: Acute phase, parasitemia, biological cycle, clinical-pathological aspects and treatment. Ex Parasitol, v. 128, p. 347-352, 2011.

DA COSTA AP, COSTA FB, SOARES HS, RAMIREZ DG, MESQUITA ETKC, GENNARI SM, MARCILI A. Trypanosoma cruzi and Leishmania infantum chagasi Infection in Wild Mammals from Maranhão State, Brazil. Vector Borne Zoonotic Dis, v. 15, p. 656-666, 2015.

FIGUEIREDO FB, LIMA-JÚNIOR FEF, TOMIO JE, INDÁ FMC, CORRÊA GLB, MADEIRA MF. Leishmaniose Visceral Canina: Dois casos autóctones no município de Florianópolis, estado de Santa Catarina. Acta Sci Vet, v. 40, p. 1-4, 2012.

GALVIS-OVALLOS F, DA SILVA MD, BISPO GBDS, DE OLIVEIRA AG, NETO JRG, MALAFRONTE RS, GALATI EAB. Canine visceral leishmaniasis in the metropolitan area of São Paulo: Pintomyia fischeri as potential vector of Leishmania infantum. Parasite, v. 24, 2017.

GONTIJO CMF, MELO MN. Leishmaniose Visceral no Brasil: quadro atual, desafios e perspectivas. Rev Bras Epidemiol, v. 7, p. 228-349, 2004.

GOTTLIEB J, ANDRÉ MR, SOARES JF, GONÇALVES LR, OLIVEIRA MT, COSTA MM, Labruna MB, Bortolini CE, Machado RZ, Vieira MIB. Rangelia vitalii, Babesia spp. and Ehrlichia spp. in dogs in Passo Fundo, state of Rio Grande do Sul, Brazil. Rev Bras Parasitol Vet, v. 25, p. 172-178, 2016.

HIRAMOTO RM, OLIVEIRA SS, RANGEL O, HENRIQUES LF, TANIGUCHI HH, BARBOSA JER, CASANOVA C, VIVIANI JUNIOR A, SAMPAIO SMP, SPINOLA R, REHDER S, LINDOSO JAL, TOLEZANO JE. Classificação epidemiológica dos municípios do Estado de São Paulo segundo o Programa de Vigilância e Controle da Leishmaniose Visceral, 2017. Bepa, v. 16, p. 11-35, 2019.

HUNFELD K, HILDEBRANDT A, GRAY J. Babesiosis: Recent insights into an ancient disease. ‎Int J Parasitol, v. 38, p. 1219-1237, 2008.

LABRUNA MB. Biologica-ecologia de Rhipicephalus sanguineus (Acari: Ixodidae). Rev Bras Parasitol Vet, v. 13, p. 123-124, 2004.

LABRUNA MB, JORGE RSP, SANA DA, JÁCOMO ATA, KASHIVAKURA CK, FURTADO MM, FERRO C, PEREZ SA, SILVEIRA L, SANTOS JÚNIOR TS, MARQUES SR, MORATO RG, NAVA A, ADANIA CH, TEIXEIRA RHF, GOMES AAB, CONFORTI VA, AZEVEDO FCC, PRADA CS, SILVA JCR, BATISTA AF, MARVULO MFV, MORATO RLG, ALHO CJR, PINTER A, FERREIRA PM, FERREIRA F, BARROS-BATTESTI, DM. Ticks (Acari: Ixodida) on wild carnivores in Brazil. Exp Appl Acarol, v. 36, p. 149-163, 2005.

LAINSON R, RANGEL EF. Lutzomyia longipalpis e a eco-epidemiologia da leishmaniose visceral americana, com particular referência ao Brasil: uma revisão. Mem Inst Oswaldo Cruz, v. 100, p. 811-827, 2005.

LEMOS TD, TOMA HK, ASSAD RQ, DA SILVA AV, CORRÊA RGB, ALMOSNY NRP. Clinical and hematological evaluation of Rangelia vitalii-naturally infected dogs in southeastern Brazil. Rev Bras Parasitol Vet, v. 26, p. 307-313.

LEMOS TD, CERQUEIRA AMF, TOMA HK, SILVA AV, CORRÊA RGB, PALUDO GR, MASSARD CL, ALMOSNY NRP. Detecção e caracterização molecular de piroplasmas em cães naturalmente infectados no Sudeste do Brasil. Rev Bras Parasitol Vet, v. 21, p. 137-142, 2012.

LINDOSO JAL, LINDOSO AA. Neglected tropical diseases in Brazil. Rev Inst Med Trop, v. 51, p. 247-253, 2009.

LORETTI AP, BARROS SS. Infecção por Rangelia vitalli (“nambiuvú”, “peste de sangue”) em caninos: revisão. MEDVEP, v. 2, p. 128-144, 2004.

MARCILI A, SPERANÇA MA, COSTA AP, MADEIRA MF, SOARES HS, SANCHES COCC, ACOSTA ICL, GIROTTO A, MINERVINO AHH, HORTA MC, SHAW JJ, GENNARI SM. Phylogenetic relationships of Leishmania species based on trypanosomatid barcode (SSU rDNA) and gGAPDH genes: Taxonomic revision of Leishmania (L.) infantum chagasi in South America. Infect Genet Evol, v. 25, p. 44-51, 2014.

MASSARD CL, FONSECA AH. Carrapatos e doenças transmitidas comuns ao homem e aos animais. AHV, v. 135, p. 15-23, 2004.

MONGRUEL ACB, IKEDA P, SOUSA KCM, BENEVENUTE JL, FALBO MK, MACHADO RZ, CARRASCO AOT, ANDRÉ MR, SEKI MC. Molecular detection of vector borne pathogens in anemic and thrombocytopenic dogs in southern Brazil. Ver, v. 27, p. 505-513, 2018.

MORAES-FILHO J, MARCILI A, NIERI-BASTOS FA, RICHTZENHAIN LJ, LABRUNA MB. Genetic analysis of ticks belonging to the Rhipicephalus sanguineus group in Latin America. Acta Tropica, v. 117, p. 51-55, 2011.

O'DWYER LH, LOPES VV, RUBINI AS, PADUAN KS, RIBOLLA PEM. Babesia spp. infecção em cães de áreas rurais do estado de São Paulo, Brasil. Rev Bras Parasitol Vet, v. 18, p. 23-26, 2009.

OLIVEIRA LPI, CARDOZO GPI, SANTOS EV, MANSUR MAB, DONINI, IAN, ZISSOU VG, ROBERTO PG, MARINS M. Molecular analysis of the rRNA genes of Babesia spp and Ehrlichia canis detected in dogs from Ribeirão Preto, Brazil. Braz J Microbiol, v. 40, p. 238-240, 2009.

PELEG O, BANETH G, EYAL O, INBAR J, HARRUS SHIMON. Multiplex real-time qPCR for the detection of Ehrlichia canis and Babesia canis vogeli. Vet Parasitol, v. 173, p. 292-299, 2010.

PINTER A, COSTA CS, HOLCMAN MM, CAMARA M, LEITE RM. A Febre Maculosa Brasileira na Região Metropolitana de São Paulo. São Paulo, SP: Secretaria de Estado da Saúde de São Paulo. Bepa, v. 13, p. 3-47, 2016.

QUADROS RM, SOARES JF, XAVIER JS, PILATI C, COSTA JL, MIOTTO BA, MILETTI LC, LABRUNA MB. Natural Infection of the Wild Canid Lycalopex gymnocercus by the Protozoan Rangelia vitalii, the Agent of Canine Rangeliosis. J Wildl Dis, v. 51, p. 787-789, 2015.

RIBEIRO CR, GONÇALVES CA, CRUZ LM, GALERA PD. Prevalência da leishmaniose visceral canina e coinfecções em região periurbana no Distrito Federal – Brasil. Ciênc Anim Bras, v. 20, p. 1-8, 2019.

SAVIĆ S, VIDIĆ B, GRGIĆ Z, POTKONJAK A, SPASOJEVIC L. Emerging vector-borne diseases – incidence through vectors. Front Public Health, v.2, 2014.

SCHÖNIAN G, NASEREDDIN A, DINSE N, SCHWEYNOCH C, SCHALLIG HD, PRESBER W, JAFFE CL. PCR diagnosis and characterization of Leishmania in local and imported clinical samples. Diagn Microbiol Infect Dis, v. 47, p. 349-358, 2003.

SILVA FS. Patologia e patogênese da leishmaniose visceral canina. Revista Trópica, v. 1, p. 20, 2007.

SILVA RE, SAMPAIO BM, TONHOSOLO R, DA COSTA AP, COSTA LES, NIERI-BASTOS FA, SPERANÇA MA, MARCILI A. Exploring Leishmania infantum cathepsin as a new molecular marker for phylogenetic relationships and visceral leishmaniasis diagnosis. Bmc Infect Dis, v.19, 2019.

SOARES JF, CARVALHO L, MAYA L, DUTRA F, VENZAL JM, LABRUNA MB. Molecular detection of Rangelia vitalii in domestic dogs from Uruguay. Vet Parasitol, v. 210, p. 98-101, 2015.

SOARES JF, COSTA FB, GIROTTO-SOARES A, DA SILVA AS, FRANÇA RT, TANIWAKI AS, DALL'AGNOL B, RECK J, HAGIWARA MK, LABRUNA MB. Evaluation of the vector competence of six ixodid tick species for Rangelia vitalii (Apicomplexa, Piroplasmorida), the agent of canine rangeliosis. Ticks Tick Borne Dis, v. 9, p. 1221-1234, 2018.

SPOLIDORIO MG, TORRES MM, CAMPOS WNS, MELO ALT, IGARASHI M, AMUDE AL, LABRUNA MB, AGUIAR DM. Detecção molecular de Hepatozoon canis e Babesia canis vogeli em cães domésticos de Cuiabá, Brasil. Rev Bras Parasitol Vet, v. 20, p. 253-255, 2011.

VON ZUBEN APB, DONALÍSIO MR. Dificuldades na execução das diretrizes do Programa de Vigilância e Controle da Leishmaniose Visceral em grandes municípios brasileiros. Cad Saúde Pública, v. 32, 2016.




DOI: https://doi.org/10.34117/bjdv6n6-191

Refbacks

  • There are currently no refbacks.