Efecto de la adición de bacterias nitrificantes autóctonas en la formación de bioflococs para la mejoría de la calidad del agua de cultivo de organismos acuáticos / Effect of the addition of native nitrifying bacteria in the formation of bioflocs for the improvement of the quality of the water of culture of aquatic organisms

Jéssica Lucinda Saldanha da Silva, Marina Teresa Torres Rodríguez, Oscarina Viana de Sousa

Abstract


La intensificación de los sistemas de cultivos acuícolas ocasiona el acumulo de materia orgánica y compuestos nitrogenados, conduciendo al deterioro de la calidad del agua y las pérdidas zootécnicas de los animales confinados. Para la solución de estos problemas se ha aplicado la tecnología de bioflocos, proporcionando un entorno bioseguro. El objetivo del presente trabajo fue evaluar el proceso de formación de flocos a partir de la adición de un consorcio de bacterias nitrificantes autóctonas y su posterior desarrollo en un sistema de cultivo acuícola. El trabajo fue desarrollado en dos etapas: Formación de bioflocos en el laboratorio y posterior aplicación del biofloco domesticado en una granja de cultivo. Se determinaron variables físico-químicas, monitoreo microscópico de los flocos y análisis microbiológico. Se pudo observar un aumento y estabilización en la cantidad de agregados microbianos con la adición de bacterias nitrificantes en relación con el biofloco espontáneo in vitro. Fue detectado un aumento en la cuantificación de bacterias heterotróficas y sólidos sedimentables en la primera semana de inoculación en la granja de cultivo. Después de 20 días de cultivo, fue posible observar una disminución en el amonio, mientras que las concentraciones de nitrito solo se estabilizaron después de 100 días de cultivo. Las tilapias presentaron una supervivencia mayor del 90% y un incremento de 1.83 g/día. Fue verificada la eficiencia del proceso de formación de bioflocos inducidos con la utilización de un consorcio de bacterias nitrificantes autóctonas en el laboratorio y su aplicación en el cultivo de Tilapia.

 

 


Keywords


Variables físico-químicas, exopolysaccharide, antagonism, enzimas extracelulares.

References


AVNIMELECH, Y. Feeding with microbial flocs by tilapia in minimal discharge bio-flocs technology ponds. Aquaculture, v. 264, p. 140–147, 2007.

AVNIMELECH, Y.; MOKADY, S.; SCHROEDER, G.L. Circulated ponds as efficient bioreactors for single-cell protein production. Israeli Journal Aquaculture Bamidgeh, v. 41, n. 2, p. 58–66, 1989.

AVNIMELECH, Y. Biofloc Technology: A Practical Guide Book. 2nd: 50-67., World Aquaculture Society, Baton Rouge, USA, 2009.

AHMAD, I.; RANI, A. M.B.; VERMA, A. K.; MAQSOOD, M. Biofloc technology: an emerging avenue in aquatic animal healthcare and nutrition. Aquaculture International, v. 25, p. 1215–1226, 2017.

APHA. American Public Health Association. 2000. Standard Methods for the Examination of Water and Waste Water. 19th ed. Washington, DC. American Public Health Association, 1-10 p.

BENTZON-TILIA, M.; SONNENSCHEIN, E. C.; GRAM, L. Monitoring and managing microbes in aquaculture – Towards a sustainable industry. Biotechnology, v. 9, p. 576–584, 2016.

BURFORD, M. A.; THOMPSON, P. J.; MCINTOSH, R. P.; BAUMAN, R. H.; PEARSO, D. C. Nutrient and microbial dynamics in high-intensity, zero-exchange shrimp ponds in Belize. Aquaculture, v.219, n. 1, p. 393-411, 2003.

BIDDANDA, B.A.; POMEROY, L.R. Microbial aggregation and degradation of phytoplankton-derived detritus in seawater. I. Microbial succession. Marine Ecology – Progress Series, v. 42, p. 79-88, 1988.

CRAB, R.; AVNIMELECH, Y.; DEFOIRDT, T.; BOSSIER, P.; VERSTRAETE, W. Nitrogen removal in aquaculture for a sustainable production. Aquaculture, v. 270. n. 1–4, p. 1-14, 2007.

CRAB, R.; DEFOIRDT, T.; BOSSIER, P.; VERSTRAETE, W. Biofloc technology in aquaculture: Beneficial effects and future challenges. Aquaculture, 356-357: 351-356, 2012.

CHRISTENSEN, G. D.; SIMPSON, W. A.; YOUNGER, J. J.; BADDOUR, L. M.; BARRETT, F.F.; MELTON, D. M. Adherence of coagulase- negative staphylococci to plastic tissue culture plates: a quantitative model for the adherence of staphylococci to medical devices. Journal of Clinical Microbiology, v. 22, p. 996-1006, 1985.

CLESCERI, L. S.; GREENBERG, A. E.; EATON, A. D. Standard methods for the examination of water and wastewater (20thed.). Washington, DC: American Public Health Association, 1998.

CORREIA, E. S.; WILKENFELD, J. S.; MORRIS, T. C.; PRANGNELL, E. L.; & D.I. SAMOCHA, T.M. Intensive nursery production of the Pacific white shrimp Litopenaeus vannamei using two commercial feeds with high and low protein content in a biofloc-dominated system. Aquacultural Engineering, v. 59, p. 48-54, 2014.

DANIEL, N.; NAGESWARI, P. Exogenous Probiotics on Biofloc Based Aquaculture: A Review. Current Agriculture Research Journal, v. 5, n. 1, p. 88-107, 2017.

DOWNES, M. P.; ITO, K. Compendium of methods for the microbiological examination of foods. APHA. 4th ed.Washington, DC, 2001.

EMERENCIANO, M.; GAXIOLA, G.; CUZON, G. Biofloc Technology (BFT): A Review for Aquaculture Application and Animal Food Industry. In: Biomass Now: Cultivation and Utilization, Matovic, M.D. (Ed.). Chapter 12, InTech, Rijeka, Croatia, pp: 302-328, 2013.

EMERENCIANO, M. G. C.; MARTÍNEZ-CÓRDOVA, L. R.; MARTÍNEZ-PORCHAS, M.; MIRANDA-BAEZA, A. Biofloc Technology (BFT): A Tool for Water Quality Management in Aquaculture. In: H. Tutu (Ed.). Water Quality. InTech, doi: 10.5772/66416, 2017.

FREEMAN, D. J.; FALKINER, F. R.; KEANE, C. T. New method for detecting slime production by coagulase negative staphylococci. Journal of Clinical Pathology, v. 42, p. 872-874, 1989.

FURNISS, A. L.; LEE, J. V.; DONOVAN, T. J. The Vibrio. Monograph Series, London: Public Health Laboratory Service, p. 58, 1979.

GAONA, C. A. P.; POERSCH, L. H.; KRUMMENAUER, D.; FOES, G. K.; WASIELESKY JUNIOR, W. 2011. The effect of solids removal on water quality, growth and survival of litopenaeus vannamei in a biofloc technology culture system. International Journal of Recirculating Aquaculture, 12:54-73.

GAONA, C. A. P.; ALMEIDA, M. S.; VIAU, V.; POERSCH, L. H.; WASIELESKY JUNIOR, W. Effect of different total suspended solids levels on a Litopenaeus vannamei (Boone, 1931) BFT culture system during biofloc formation. Aquaculture Research, v. 48, p. 1070–1079, 2017.

KASAN, N.A.; GHAZALI, N. A.; IKHWANUDDIN, M. H. D.; IBRAHIM, Z. Isolation of Potential Bacteria as Inoculum for Biofloc Formation in Pacific Whiteleg Shrimp, Litopenaeus vannamei Culture Ponds. Pakistan Journal of Biological Sciences, v. 20, n. 6, p. 306-313, 2017.

LARA, G.; KRUMMENAUER, D.; ABREU, P. C.; POERSCH, L. H.; WASIELESKY JR, W. The use of different aerators on Litopenaeus vannamei biofloc culture system: effects on water quality, shrimp growth and biofloc composition. Aquaculture International, v. 25, p. 147–162, 2017.

LIMA, E. C.R.; SOUZA, R. L.; GIRAO, P. J. M.; BRAGA, I. F. M.; CORREIA, E. S. Culture of Nile tilapia in a biofloc system with different sources of Carbono. Revista Ciência Agronômica, v. 4, n. 3, p. 458-466, 2018.

LIU, P. C.; LEE, K. K.; CHEN, S. N. Pathogenicity of different isolates of Vibrio harveyi in tiger prawn, Penaeus monodon. Letter in Applied Microbiology, v. 22, p. 413-416, 1996.

MARTÍNEZ-PORCHAS, M.; EZQUERRA-BRAUER, M.; MENDOZA-CANO, F.; CHAN-HIGUERA, J. E.; VARGAS-ALBORES, F.; MARTÍNEZ-CORDOVA, L. R. Effect of supplementing heterotrophic and photoautotrophic biofloc, on the production response, physiological condition and post-harvest quality of the whiteleg shrimp, Litopenaeus vannamei. Aquaculture Reports, v. 16, 2020.

MARTINEZ-CORDOVA, L. R.; MARTINEZ-PORCHAS, M.; EMERENCIANO, M.; MIRANDA-BAEZA, A.; GOLLAS, T. From microbes to fish the next revolution in food production. Critical Reviews in Biotechnology, v. 37, p. 287-295, 2016.

MORO, E. B.; PESSINI, J. E.; YAMASHIRO, D.; NEU, D. H.; BITTENCOURT, F.; BOSCOLO, W. R.; SIGNOR, A. Fenilalanina em dietas para juvenis de tilápia do Nilo. Brazilian Journal of Development, v. 6, n.5, p. 29340-29353, 2020.

PÉREZ-FUENTES, J. A.; HERNÁNDEZ-VERGARA, M. P.; PÉREZ-ROSTRO, C. I.; FOGEL. I. C: N ratios affect nitrogen removal and production of Nile tilapia Oreochromis niloticus raised in a biofloc system under high density cultivation. Aquaculture, v. 425, p. 247–251, 2016.

RAY A. J.; LEWIS, B. L.; BROWDY, C. L.; LEFFLER, J. W. Suspended solids removal to improve shrimp (Litopenaeus vannamei) production and an evaluation of a plant-based feed in minimal exchange, superintensive culture systems. Aquaculture, v. 299, p. 89-98, 2010.

RODRIGUES D. P.; RIBEIRO, R. V.; ALVES, R. M.; HOFER, E. Evaluation of virulence factors in environmental isolates of Vibrio species. Memórias do Instituto Oswaldo Cruz, v. 88, n. 4, p. 589-592, 1993.

SÁ, M. V. C. 2012. Limnocultura: Limnologia para aquicultura. Fortaleza: Edições UFC. 218 pp.

SANTOS, N. B. V.; FURTADO, P. S.; CÉSAR, D. E.; WASIELESKY JÚNIOR, W. Assessment of the nitrification process in a culture of pacific White shrimp, using artifical substrate and bacterial inoculum in a biofloc technology system (BFT). Ciência Rural, v. 49, n. 6, p.1-10, 2019.

SILVA, L.; ESCALANTE, E.; VALDÉS-LOZANO, D.; HERNÁNDEZ, M.; GASCA-LEYVA, E. Evaluation of a Semi-Intensive Aquaponics System, with and without Bacterial Biofilter in a Tropical Location. Sustainability, v. 9, p. 592-604, 2017.

SILVA, L.; FALCON, D.R. PESSÔA, M.N.C.; CORREIA, E.S. Carbon sources and C:N ratios on water quality for Nile tilápia farming in biofloc system. Revista Caatinga, Mossoró, v. 30, n. 4, p. 1017-1027, 2017.

SOUZA, J.; ALESSANDRO, C.; WASIELESKY JR , W.; PAULO, A. C. Does the biofloc size matter to the nitrification process in Biofloc Technology (BFT) systems? Aquaculture, v. 500, p. 443–450, 2019.

TANSEL, B. Morphology, composition and aggregation mechanisms of soft bioflocs in marine snow and activated sludge: A comparative review. Journal of Environmental Management, v. 205, p. 231-243, 2018.

TEATHER, R. M.; WOOD, P. J. Use of Congo Red-Polysaccharide Interactions in Enumeration and Characterization of Cellulolytic Bacteria from the Bovine Rument. Applied and Environmental Microbiology, v. 43, n. 40, p.777-780, 1982.




DOI: https://doi.org/10.34117/bjdv6n6-078

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