Análise in silico de Catepsina L-like de Leishmania braziliensis: busca de um novo alvo para o diagnóstico molecular / Analysis in silico of Catepsin L-like of Leishmania braziliensis: search for new target for molecular diagnosis

David Volfchuk Markus, Ryan Emiliano da Silva, Jaciara de Oliveira Jorge Costa, Roberta Carvalho de Freitas e Azevedo, Danilo Ciccone Miguel, Arlei Marcili

Abstract


 

A Leishmaniose Tegumentar Americana é uma importante zoonose causada por diferentes espécies de Leishmania, sendo Leishmania (Viannia) braziliensis a principal delas. Estes parasitos possuem animais silvestres e domésticos como reservatórios conhecidos. A forma tegumentar de Leishmaniose está difundida no território brasileiro e, apesar de acarretar um prognóstico menos grave do que a forma visceral, é de extrema importância por possuir potencial lesivo às mucosas e à pele, trazendo também uma grande carga emocional, afetando socialmente a vida dos pacientes. Neste estudo, realizamos a análise in silico das sequências de catepsina L-like a fim de padronizar um diagnóstico molecular sensível e específico para L. (V.) braziliensis. A busca por iniciadores para o diagnóstico molecular específico foi realizada investigando-se características físico-químicas e análise in silico da especificidade dos segmentos escolhidos através dos valores de similaridade com bancos de dados (Nucleotide Basic Local Alignment Search Tool (BLASTn)). Os resultados do delineamento in silico levaram à escolha dos seguintes iniciadores: CatLeishBF; CatLeishBR; CatLeishBLF; CatLeishBLR e Calbra. A análise in silico destes iniciadores demonstrou 100% de similaridade com L. (V.) brazilensis, não sendo observada complementaridade com sequências de outras espécies depositadas no banco de dados. Os testes in vitro, contudo, não apresentaram amplificações específicas. Novos estudos devem ser conduzidos a fim de aprimorarmos o diagnóstico das leishmanioses tegumentares para medidas terapêuticas mais efetivas e precoce.

 


Keywords


Leishmania; Lutzomyia; lesões cutâneas; proteases; PCR

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DOI: https://doi.org/10.34117/bjdv6n6-036

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