Formulation development of an herbal hand sanitizer containing Moringa olifera silver nanoparticles / Desenvolvimento da formulação de um higienizador de mãos à base de ervas contendo nanopartículas de prata Moringa olifera

Oyeniyi Y.J, Mumuni A.M

Abstract


Background: The antimicrobial activity of Moringa olifera methanol leaf extract had been reported but with no effort to develop the extract into useful pharmaceutical products that is clinically relevant Objectives: This study aimed to develop an alcohol base herbal hand sanitizer containing synthesized Moringa silver nanoparticles as the active ingredient for use in personal hygiene and for combating the spread of outbreak of communicable diseases Methods: 10 mL of  Moringa leaf extract (MLE), obtained by macerating the dried leaves with methanol was reacted with (40,30,20 and 10 mL) of 1mM silver nitrate solution to produced batches of Moringa silver nanoparticles (MSN). The morphology of MSN was obtained using a scanning electron microscope, while the particle sizes, polydispersity index and the zeta potential were obtained using a ZS-90 Zetasizer with dynamic and electrophoretic light scattering capabilities. MSN antimicrobial action was evaluated by agar diffusion method sequence to formulation of the herbal hand sanitizers, which were the evaluated for their effectiveness to reduce the microbial population. Results and Discussion: The color changes indicating the formation of MSN were within 10 minutes, all other MSN parameters significantly varied from batch to batch,(p ≤ 0.05) indicating the need of process optimization. The MSN were moderately dispersed, negatively charged and stable with PDI and ZP values ranging, 0.11-0.39 and 22- 33 mV respectively. FA and FB with yields above 50 % and mean particle sizes of about 30 and 38 nm were selected for production scale up and formulation studies. The percentage microbial death for FA1 and FB1 were 100 % showing an improvement above the standard of 99.9 % microbial death. Conclusion: Alcohol base herbal hand sanitizers were successful formulated with synthesized MSN which demonstrated an improvement above the industrial standard with ability to eradicate microbial load by 100 %. These novel herbal hand sanitizers could be readily deployed to combat the spread of communicable disease outbreak like the current covid-19 pandemic.


Keywords


antimicrobial activity, green synthesis. Hand sanitizing and Moringa olifera  

References


A. Leone, A. Spada, A. Battezzati, A. Schiraldi. et al. Cultivation, Genetic, Ethnopharmacology, Phytochemistry and Pharmacology of Moringa oleifera Leaves: An Overview. Int J Mol Sci. 16(6):12791-835, 2015.

A. Leone, A. Spada, A. Battezzati, A. Schiraldi et al. Moringa oleifera Seeds and Oil: Characteristics and Uses for Human Health. Int J Mol Sci 17(12): 2140-14, 2016

K. Ajit, B.K. Choudhary, N.G. Bandyopadhyay. Comparative evaluation of hypoglycaemic activity of some Indian in alloxan diabetic rats. J Ethnopharmacol, 84, 105-8, 2002.

N.K. Amaglo, R.N. Bennet, R.B.L. Curto et al. selected phytochemicals and nutrients in different tissues of the multipurpose tree Moringa oleifera L., grown in Ghana. J of Food Chem, 122, 1047-54, 2010.

W.J. Fahey. Moringa oleifera: a review of the medical evidence for its nutritional, therapeutic, and prophylactic properties. Part 1. Trees Life J. 1, 1–24, 2005.

A. Leone, G. Fiorillo, F. Criscuoli, S. Ravasenghi et al. Nutritional characterization of phenolic profiling of Moringa oleifera leaves grown in Chad, Sahrawi refugee camps, and Haiti. Int. J. Mol. Sci. 15, 18923–18937, 2015a. doi: 10.3390/ijms160818923

J.O. Popoola, O.O. Obembe, Local knowledge, use pattern and geographical distribution of Moringa oleifera Lam. (Moringaceae) in Nigeria. J. Ethnopharmacol. 150, 682–691, 2013. doi: 10.1016/j.jep.2013.09.043

K.T. Mahmood, T. Mugal, I.U Haq. Moringa oleifera: a natural gift - a review. J. Pharm. Sci Res. 2, 775–781, 2010.

F.C. Maiyo, R. Moodley, and M. Singh. Cytotoxicity, antioxidant and apoptosis studies of quercetin-3-O-glucoside and 4-(beta-Dglucopyranosyl-1->4-alpha-L-rhamnopyranosyloxy)-benzyl isothiocyanate from Moringa oleifera. Anticancer. Agents Med. Chem. 16, 648–656, 2016. doi: 10.2174/1871520615666151002110424

M.A Ibrahim, A. Mohammed, M.B. Isah, and A.B. Aliyu. Antitrypanosomal activity of African medicinal plants: a review update. J. Ethnopharmacol. 154, 26–54, 2014. doi: 10.1016/j.jep.2014.04.012.

A. Gupta, M.K. Gautam, R.K. Singh, M.V. Kumar, et al. Immunomodulatory effect of Moringa oleifera Lam. extract oncyclophosphamide induced toxicity in mice. Indian J. Exp. Biol. 48, 1157–1160, 2010.

V. Lipipun, M. Kurokawa, R. Suttisri, P. Taweechotipatr, et al. Efficacy of Thai medicinal plant extracts against herpes simplex virus type 1 infection in vitro and in vivo. Antiviral Res. 60, 175–180, 2003. doi: 10.1016/S0166-3542(03)00152-9.

M. Minaiyan, G. Asghari, D. Taheri, M. Saeidi, and S. Nasr-Esfahani. Anti-inflammatory effect of Moringa oleifera Lam. seeds on acetic-induced acute colitis in rats. Avicenna J. Phytomed. 4, 127–136, 2014.

V.K. Vidhu, A. Aromal, D. Philip. Green synthesis of silver nanoparticles using Macrotyloma uniflorum. Spectrochim. Acta A Mol Biomol Spectros 2011 83:392–397, 2011.

R. Veerasamy, T.Z. Xin, S. Gunasagaran, T.F. Xiang et al. Biosynthesis of silver nanoparticles using mangosteen leaf extract and evaluation of their antimicrobial activities. J Saudi Chemical Society 15:113–120, 2011.

A. Lateef, S.A. Ojo, M.A. Azeez, T.B. Asafa et al, Cobweb as novel biomaterial for the green and eco-friendly synthesis of silver nanoparticles Appllied Nanoscience, 6; 863-874, 2016

H. Katas, L. Chei, A. Sin, F. Buang et al. Antibacterial activity of biosynthesized gold nanoparticles using biomolecules from Lignosus rhinocerotis and chitosan. Saudi Pharmaceutical Journal 27(2) 283-292, 2018.

E.A. Adebayo, J.B. Ibikunle, A.M. Oke, A. Lateef et al. Antimicrobial and antioxidant activity of Silver, Gold and Silver-Gold Alloy Nanoparticles phytosynthesized using extract of Opuntia ficus-indica Rev. Adv. Mater. Sci. 2019; 58:313–326.

World Health Organization. Shortage of personal protective equipment endangering health workers worldwide. https://www.who.int/news-room/detail/03-03-2020-shortage-of-personal-protective-equipment-endangering-health-workers-worldwide. 2020

World Health Organization. WHO guidelines on hand hygiene in health care. First Global Patient Safety challenge. Clean Care is SaferCare. Geneva: WHO; 2009.

S.W. Nandkishor. Formulation and Evaluation of Herbal Sanitizer. Int. J. PharmTech Res, 5(1) 2013.

G.E. Trease and W.C. Evans. Pharmacognosy. Editied by Evans, WC: A textbook of Pharmacognosy.(16th Edition) Builler Tindall and Causel, London 2010.

P. Napoleon. J. Anitha , R.R. Emilin. Isolation, analyses and identification of phytochemical and antimicrobial activity of Moringa oleifera Lam. Current Biotica 3:33-39, 2009.

M. Pandurangan, P.C. Nagajyothi, D.H. Kim et al. Green Synthesis and Characterization of Biologically Active Silver Nanoparticles Using Perilla frutescens Leaf Extract. J Clust Sci 28, 81–90 2017.

G.M. Sulaiman, W.H. Mohammed, T.R. Marzoog. Green synthesis, antimicrobial and cytotoxic effects of silver nanoparticles using Eucalyptus chapmaniana leaves extract. Asian Pacific Journal of Tropical Biomedicine 3 (1) 58-63, 2013

H. Katas, C.S. Lim, A.Y. Hamdi, N. Azlan, et al., Antibacterial activity of biosynthesized gold nanoparticles using biomolecules from Lignosus rhinocerotis and chitosan Saudi Pharmaceutical Journal 27 :283–292, 2019.

K. Raja, A. Saravanakumar, R. Vijayakumar. Efficient synthesis of silver nanoparticles from Prosopis juliflora leaf extract and its antimicrobial activity using sewage Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy 97:490-4, 2012.

M. Shaik, M. Khan, M. Kuniyil, A. Al-Warthan, et al. Plant-Extract-Assisted Green Synthesis of Silver Nanoparticles Using Origanum vulgare L. Extract and Their Microbicidal Activities. Sustainability, 10(4), 913, 2018.

F. Nickon, Z.A. Saud, M.H. Rehman, M.E. Haque, In-vitro antimicrobial activity of the compound isolated from chloroform extract of M. oleiferaLam. Pak. J. Biol. Sci. 22:1888 – 1890, 2003.

A. Caceres, O. Cabrera, O. Morales, P. Mollinedo, P. Mendiab. Pharmacological properties of Moringa oleifera 1: Preliminary screening for antimicrobial activity Journal of Ethnopharmacology , 33: 213-2 I6, 1991.

L.S. Devi, S.R. Joshi. Antimicrobial and synergistic effects of silver nanoparticles synthesized using: soil fungi of high altitudes of Eastern Himalaya. Mycobiol 40:27–34, 2012.

Technical bulletin on BacTiter-Glo™ Microbial Cell Viability Assay https://www.promega.com/-/media/files/resources/protocols/technical-bulletins/101/bactiter-glo-microbial-cell-viability-assay-protocol.pdf?la=en

K. Raja, A. Saravanakumar, R. Vijayakumar. Efficient synthesis of silver nanoparticles from Prosopis juliflora leaf extract and its antimicrobial activity using sewage Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 97: 490–494, 2012.

S. Zaki, M.F. El-Kady, D. Abd-El-Haleem. Biosynthesis and structural characterization of silver nanoparticles from bacterial isolates. Mater Res Bull 46:1571–1576, 2011.

A Lateef, I.A. Adelere, E.B.Gueguim-Kana, T.B. Asafa, L.G. Beukes. Green synthesis of silver nanoparticles using keratinase obtained from a strain of Bacillus safensis LAU 13. Int Nano Lett 5:29–35, 2015a .

R.R.R. Kannan, R. Arumugam, D. Ramya, K. Manivannan , P. Anantharaman. Green synthesis of silver nanoparticles using marine macroalga Chaetomorpha linum. Appl Nanosci 3:229–233, 2013.

A Lateef, S.A. Ojo, M.A. Azeez, T.B.Asafa . Cobweb as novel biomaterial for the green and eco-friendly synthesis of silver nanoparticles Appl Nanosci 6:863–874,2015.

Y.J. Oyeniyi, A. Abdulsamad. Fabrication and Evaluation of Multiple drugs-Loaded Liposome for the Management of HR positive Breast Cancer. Nig. J. Pharm Res, 13(2) 83-95, 2017.

S. Ankanna, T.N.V.K.V. Prasad, E.K. Elumalai , N. Savithramman

Production of biogenic silver nanoparticle using Boswelllia ovalifoliolata stem bark

Digest Journal of Nanomaterials and Biostructures 5:2: 369 – 372, 2010.

S . Bhattacharjee. DLS and Zeta potential : what they are and what they are not? Journal of Controlled Release 235: 337-51, 2016.

H. K Ardani, C. Imawan, W. Handayani, D. Djuhana et al Enhancement of the stability of silver nanoparticles synthesized using aqueous extract of Diospyros discolor Willd. leaves using polyvinyl alcohol International Symposium on Current Progress in Functional Materials. IOP Conf. Series: Materials Science and Engineering 188: 012056, 2017.

S. Bhattacharjee, L.H. De-Haan, N.M. Evers, X Jiang. et al.

Role of surface charge and oxidative stress in cytotoxicity of organic monolayer-coated silicon nanoparticles towards macrophage NR8383cells, Part. Fibre Toxicol.; 7 : 25. 2010

P. M. Favi, M. Gao, L S.P. Johana -Sepúlveda Arango, S.P. Ospina, et al. Shape and surface effects on the cytotoxicity of nanoparticles: gold nanospheres versus gold nanostars, J. Biomed. Mater. Res. A 103: 3449–3462, 2015.

A. Saxena, RM Tripathi, F Zafar, P Singh. Green synthesis of silver nanoparticles using aqueous solution of Ficus benghalensis leaf extract and characterization of their antibacterial activity Materials Letters (67) 91–94. 2012

P. Prakash, P. Gnanaprakasam, R .Emmanuel, S. Arokiyara, et al. Synthesis of silver nanoparticles from leaf extract of Mimusops elengi, Linn. for enhanced antibacterial activity against multi drug resistant clinical isolates Colloids and Surfaces B: Biointerfaces (108) 255– 259, 2013.

D.D. Blaney, E.R Daly, K.B Kirkland, J.E. Tongren, et al. Control use of alcohol based hand sanitizers as a risk factor for norovirus outbreaks in long term facilities in northern New England. Am J Infect, 39 (4): 296-301, 2011.

CDC (Centers for Disease Control and Prevention). Guideline for Hand Hygiene in Health-Care Settings: Recommendations of the Healthcare Infection Control Practices Advisory Committee and the HICPAC/SHEA/APIC/IDSA Hand Hygiene Task Force. MMWR., 51: 1-56, 2002

J. J. Omogbai, C.C Azodo, A.O Ehizele, A. Humo Hand hygiene amongst dental professional in a tertiary dental clinic. Afr J Clin Exp Microbiol., 12(1): 9-14,2011

Y.J. Oyeniyi, A.J. Yusuf Quantitative effects of formulation process variables on antitumor activities of doxorubicin. Braz J Technol. 2(4) 967-983 2020

S.T. Chambers, B Peddie, A Pithie. Ethanol disinfection of plastic-adherent micro-organisms. J Hospital Infect. (63) 193–196. 2006




DOI: https://doi.org/10.38152/bjtv4n1-003

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