Methods for Candida albicans biofilm formation on temporary soft liner/ Métodos para a formação de biofilme de Candida albicans em reembasador macio temporário

Authors

  • Francine Sumie Morikava Brazilian Journals Publicações de Periódicos, São José dos Pinhais, Paraná
  • Gustavo Simão Moraes
  • Victoria Schlumberger Cachoeira
  • Mayara Precoma Ramalho
  • Rafaela Alves da Silva
  • Karin Hermana Neppelenbroek
  • Vanessa Migliorini Urban

DOI:

https://doi.org/10.34119/bjhrv4n5-234

Keywords:

Artificial saliva, Denture liners, Denture stomatitis, Dental tissue conditioning.

Abstract

This study evaluated methods for the contamination of a soft liner material (Softone) with Candida albicans biofilm. Specimens were either submitted or not to pretreatment in artificial saliva in an orbital incubator, and then held suspended in different positions (horizontal or vertical) and different storage conditions (bacteriological incubator or orbital incubator) during biofilm formation. Eight conditions were tested. All specimens were immersed in C. albicans inoculum and stored in an orbital incubator at 75 rpm or in a bacteriological incubator, both at 37ºC for 90 min. Then, they were washed in PBS, and maintained in RPMI-1640 medium under the same conditions for 48 h. The degree of contamination was determined by the XTT assay. Data were submitted to ANOVA 1-factor/Tukey HSD test (?=0.05). Specimens held horizontally in an orbital incubator showed the highest cell viability, while the ones kept vertically in a bacteriological incubator had the lowest viability (p<0.0001). The best condition for C. albicans biofilm formation was obtained when specimens were not submitted to pretreatment in saliva and were held horizontally in an orbital incubator.

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Published

2021-10-10

How to Cite

MORIKAVA, F. S.; MORAES, G. S.; CACHOEIRA, V. S.; RAMALHO, M. P.; SILVA, R. A. da; NEPPELENBROEK, K. H.; URBAN, V. M. Methods for Candida albicans biofilm formation on temporary soft liner/ Métodos para a formação de biofilme de Candida albicans em reembasador macio temporário. Brazilian Journal of Health Review, [S. l.], v. 4, n. 5, p. 21447–21459, 2021. DOI: 10.34119/bjhrv4n5-234. Disponível em: https://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/37154. Acesso em: 29 mar. 2024.

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Section

Original Papers