Predominance of Blastocystis spp, entamoeba histolytica /e.dispar and other protozoan evaluated through classical and molecular diagnostic techniques in two communities of Niterói-rj/Brazil. / Predominância de Blastocystis spp, entamoeba histolytica /e.dispar e outros protozoários avaliados através de técnicas de diagnóstico clássico e molecular em duas comunidades de Niterói-rj/Brazil

Vera Lucia Reis Belchior, Helena Keiko Toma, Keila Moreira Batista, Karine Ingrid De Souza Bernarde, Laís Gonçalves de Carvalho, Thiago Pimentel Pilotto, Yara Leite Adami, Analucia Rampazzo Xavier

Abstract


Background: Enteroparasites infections remains an important  health issue in Brazil as contaminations are still detected among inhabitants of socially vulnerable populations of the country. This study was performed in two low income communities in Niterói, Rio de Janeiro State, Brazil. Methods: Health educational actions in two selected communities were performed and in parallel, 150 stool samples were collected. Coproparasitological diagnosis was carried out employing Hoffmann, Pons & Janer , Willis and Rugai  techniques 5,6,7. Molecular diagnosis using Paglia & Visca protocol was performed to detect and differentiate E. histolytica/E.dispar infections 8. Results: Enteroparasites were found in more than 50% of the samples and protozoan infections prevailed. Blastocystis spp was the most prevalent protozoan (55.08%) followed by Endolimax nana (16.10%), Giardia lamblia (15.56%) and Entamoeba coli (11.02%). For helminth species, only Enterobius vermicularis and Strongyloides stercoralis were found, in 4.24% of positive samples. All samples were negative for E.histolytica/E. dispar infections through coproscopy, however, PCR analysis of 143 suitable stool samples showed that both species were present - as a complex or not - in 15% of the samples, in both institutions evaluated. Conclusions: The high frequency of protozoan infections may indicate extensive faecal-oral contamination. Additionally, it can ensure the basis for directed intervention actions in some communities to prevent water transmitted parasites providing sanitary infrastructure. Also, educational health programs for inhabitants of low income communities of Niterói, Rio de Janeiro State, Brazil, are necessary in order to provide a better understanding of enteroparasites and its prophylatic measures. 


Keywords


Blastocystis spp, E. histolytica/E. dispar, diagnosis

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DOI: https://doi.org/10.34119/bjhrv4n3-163

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