Efeito da Aplicação da energia vermelha monocromática (MIRE®) sobre a cicatrização de feridas cutâneas em ratos diabéticos / Monochromatic infrared energy application effect (MIRE®) of skin healing wounds in diabetic rats
DOI:
https://doi.org/10.34119/bjhrv4n2-147Keywords:
Phototherapy, Wound Healing, Diabetes MellitusAbstract
Introduction: The monochromatic infrared energy use (MIRE®) as a resource to promote healing shows excellent prospects. Objective: Determine the MIRE (890nm) application effect in skin wounds healing in diabetic rats. Methods: Experimental type study, with qualitative and quantitative sample data evaluation. Skin wounds 1 cm2 were made in the dorsal region Rattus norvegicus diabetics, who were randomly distributed in 14 animals groups and treated for seven to 14 days with 0.9% saline (control group - C) phototherapy (MIRE - wavelength = 890 nm; energy density = 24,96J / cm2; and irradiation time = 600 seconds) or clostebol acetate / neomycin sulfate (Trofodermin® - Default). Comparisons between groups were performed by ANOVA followed by post-Newman-Keuls test. Results: No statistically significant differences were found between the groups in the wounds contraction on the 4th day after wounds production. There was significantly higher wound contraction (p <0.05) in standard group (0.66 ± 0.02) compared to MIRE® (0.27 ± 0.03) and control (0.43 ± 0, 07) on the 7th monitoring day. On 14th treatment day, the wound contraction was higher (p <0.05) in standard group (0.87 ± 0.04) and MIRE® (0.81 ± 0.04) compared with control (0 68 ± 0.06). A semi-quantitative histological analysis revealed increased vascular proliferation in MIRE®. Conclusion: The results indicate that treatment with MIRE® favored the open wounds healing in diabetic rats.
References
AMORIM, E., MATIAS, J. E. F., COELHO, J. C. U., CAMPOS, A. C. L. , STAHLKE JR., H. J. S. et al . Efeito do uso tópico do extrato aquoso de Orbignya phalerata (babaçu) na cicatrização de feridas cutâneas: estudo controlado em ratos. Acta Cirurgica Brasileira, v. 21, supl. 2, pag. 67-76, 2006.
BOULTON, A. J., VILEIKYTE, L., RAGNARSON-TENNVALL, G., APELGVIST, J. The global burden of diabetic foot disease. Lancet. v. 366, n. 9498 p.1719-1724, 2005.
CAETANO, K. S.; MINATEL D. G.; SANTANA, L. A. et al. Eficácia da fototerapia associada à sulfadiazina de prata no tratamento de úlceras venosas crônicas. Fisioterapia Brasil, v. 10, n. 6, p. 388-394, 2009
CHEN, C. H., HUNG, H. S., HSU, S. . Low-energy laser irradiation increases endothelial cell proliferation, migration, and eNOS gene expression possibly via PI3K signal pathway. Lasers Surgery and Medicine, v. 40, n. 1, p. 46-54, 2008
CHILDRESS, B.B.; STECHMILLER, J. K. Role of Nitric Oxide in Wound Healing. Biological Reasearch for Nursing. v. 4, n. 1, p. 5-15, 2002
CONLAN, M. J., RAPLEY, J. W., COBB, C. M. Biostimulation of wound healing by low-energy laser irradiation. A review. Journal of Clinical Periodontology, v. 23, n. 5, p. 492-496, 1996
DADPAY, M., SHARIFIAN, Z., BAYAT, M., BAYAT, M., DABBAGH, A. Effects of pulsed infra-red low level-laser irradiation on open skin wound healing of healthy and streptozotocin-induced diabetic rats by biomechanical evaluation. Journal of Photochemistry and Photobiology, v. 111, n.1, p. 1-8, 2012
DA SILVA, A., LEAL JUNIOR, E. C., ALVES, A. C., RAMBO, C. S., DOS SANTOS, S. A. et al. Wound-healing effects of low-level laser therapy in diabetic rats involve the modulation of MMP-2 and MMP-9 and the redistribution of collagen types I and III. Journal of Cosmetic and Laser Therapy, v. 15, n. 4, p. 210-216, 2013
FAHEY, T. J.; SADATY, A.; JONES, W. G.; BARBER, A.; SMOLLER, B.; SHIRES, G. T. Diabetes impairs the late inflammatory response to wound healing. Journal of Surgery Research. v. 50, n. 4, p 308-313, 1991
GREENHALGH, D. G. Wound healing and diabetes mellitus. Clinics in Plastic Surgery. v. 30, n.1, p. 37-45, 2003
HALFOUN, V. L. R. C., Fernandes T. J., Pires, M. L. E., Braun, E., Cardozo, M. G. T., Bahbout, G. C. Estudos morfológicos e funcionais da microcirculação da pele no diabetes mellitus. Arquivos Brasileiros de Endocrinologia e Metabologia, v. 47, n. 3, 2003
HE, Y., YIP, S. L. Y., CHEUNG, K. K., HUANG, L., WANG, S., CHEING, G. L. Y. The effect of monochromatic infrared energy on diabetic wound healing. International Wound Journal. v.10, n. 6, p.645-652, 2012
HORWITZ, L. R.; BURKE, T. J., CARNEGIE, D. Augmentation of Wound Healing Using Monochromatic Infrared Energy. Advances in Wound Care. v. 12, n.1, p. 35-40, 1999
HUSSEIN, A. J., ALFARS, A.A., FALIH, M.A., HASSAN, A.A. Effects of a low level laser on the acceleration of wound healing in rabbits. North American Journal of Medical Sciences. v. 3, n. 4, p. 193-197, 2011
INTERNATIONAL DIABETES FEDERATION. Diabetes Atlas. 6. ed. Bruxelas, Bélgica, 2013
KILÍK, R., LAKYOVÁ, L., SABO, F., KRUZLIAK, P., LACJAKOVÁ, K., VASILENKO, T., VIDOVÁ, M., LONGAUER, F., RADONAK., J. Effect of equal daily doses achieved by different power densities of low-level laser therapy at 635nm on open skin wound healing in normal and diabetic rats. BioMed Research International, v. 2014, n.1, p. 1-9, 2014
KIPSHIDZE, N., NIKOLAYCHIK, V., KEELAN, M. H., Low-power helium: neon laser irradiation enhances production of vascular endothelial growth factor and promotes growth of endothelial cells in vitro. Lasers Surgery and Medicine, n. 28, n. 4, p. 355-364, 2001
LERCO, M. M.; SPADELLA, C. T.; MACHADO, J. L. M.; SCHELLINI, S. A.; PADOVANI, C. R. Caracterização de um modelo experimental de diabetes mellitus induzido pela aloxana em ratos. Estudo clínico e laboratorial. Acta Cirurgica Brasileira, v. 18, n. 2, p. 132-142, 2003.
LIM, J., SANDERS, R. A., YEAGER R. L., MILLSAP, D. S., WATKINS J.B., EELLS, J. T., HENSHEL, D. S. Attenuation of TCDD-induced oxidative stress by 670 nm photobiomodulation in developmental chicken kidney. Journal of Biochemical and Molecular Toxicology. v. 22, n. 4, p. 230-239, 2008
LOHR, N. L. KESZLER, A. PRATT, P., BIENENGRABER, M. WARLTIER, D. C., HOGG, N. Enhancement of nitric oxide release from nitrosyl hemoglobin and nitrosyl myoglobin by red/near infrared radiation: potential role in cardioprotection. Journal of Molecular and Cellular Cardiology. v. 47, n. 2, p. 256-263, 2009
LOWE, A.S.; WALKER, M.D.; O’BYRNE, M.; BAXTER, G.D.; HIRST, D.G. Effect of low-intensity monochromatic light therapy (890 nm) on a radiation-impaired, wound-healing model in murine skin. Lasers Surgery and Medicine, v. 23, n. 5, p. 291–298,1998
MACHADO, J. L. M.; MACEDO, A. R.; SILVA, M. D.; SPADELLA, C. T.; MONTENEGRO, M. R. G. Caracterização de um modelo experimental de neuropatia em ratos diabéticos induzidos por aloxana. Acta Cirurgica Brasileira, v.15, n.2, 2000
MAK, C.H., CHEING, G.L.Y. Immediate Effects of Monochromatic Infrared Energy on Microcirculation in Healthy Subjects. Photomedicine and Laser Surgery, v. 30, n. 4, p.193-199, 2012
MASSONE, F. Anestesiologia veterinária: Farmacologia e técnicas. 2. ed. Rio de Janeiro: Guanabara, p. 344, 2003
MINATEL, D. G., FRADE, M. A., FRANÇA, S .C., ENWEMEKA, C .S. Phototherapy promotes healing of chronic diabetic leg ulcers that failed do respond to other therapies. Lasers in surgery and medicine. v. 41, n. 6, p. 433-441, 2009a
MINATEL, D. G. et al . Fototerapia (LEDs 660/890nm) no tratamento de úlceras de perna em pacientes diabéticos: estudo de caso. Anais Brasileiros de Dermatologia, v. 84, n. 3, 2009b.
MIRSKY, N., KRISPEL, Y., SHOSHANY, Y., MALTZ, L. ORON, U. Promotion of angiogenesis by low energy laser irradiation. Antioxidants and Redox Signaling. v. 4, n. 5, p. 785-790, 2002
NATHER, A. et al. Anodyne therapy for recalcitrant diabetic foot ulcers: a report of four cases. Journal of Orthopaedic Surgery. v.15, n. 3, p.361-364, 2007
OLIVEIRA, S. T., LEME, M. C., PIPPI, N. L., RAISER, A. G., MANFRON, M. P. Formulações de confrei (symphytum officinale L.) na cicatrização de feridas cutâneas em ratos. Revista da faculdade de zootecnia veterinaria e agronomia, v. 7, p. 61-65, 2000
POYTON R.O., BALL K.A. Therapeutic Photobiomodulation: Nitric Oxide and a Novel Function of Mitochondrial Cytochrome C Oxidase. Discovery Medicine, v. 11, n. 57, p.154-9, 2011
POYTON, R. O., BALL, K. A., BASTELLO, P. R. Mitochondrial generation of free radicals and hypoxic signaling. Trends In Endocrinology and Metabolism. v. 20, n. 7, p. 332-340, 2009
RAHAL, S. C., ROCHA, N. S., BLESSA, E. P., IWABE, S., CROCCI, A. J. Pomada orgânica natural ou solução salina isotônica no tratamento de feridas limpas induzidas em ratos. Ciência. Rural, v. 31, n. 6, 2001
SANTOS, L. O. M. et al . Efeito da somatotropina sobre a cicatrização de feridas cutâneas, em ratos. Acta Cirurgica Brasileira. v. 17, n. 4, 2002
SCHINDL A., MERWALD, H. SCHINDL, L., KAUN, C. WOJTA, J. Direct stimulatory effect of low-intensity 670 nm laser irradiation on human endothelial cell proliferation. The British Journal of Dermatology. v. 148, n.2, p. 334-336, 2003
SHIVA, S., GLADWIN, M. T.. Shining a light on NO stores: near infrared release of NO from nitrite and nitrosylated hemes. Journal of Molecular and Cellular Cardiology. v. 46, n. 1, p. 1-3, 2009
SMITH, K. C. Laser (and LED) Therapy Is Phototherapy. Photomedicine and Laser Surgery. v. 23, n. 1, p. 78-80, 2005
SOUSA, A.P.C., PARAGUASSÚ, G.M., SILVEIRA, N.T.T., SOUZA, J. CANGUSSÚ, M.C.T., SANTOS, J.N., PINHEIRO, A.L.B. Laser and LED phototherapies on angiogenesis. Lasers in Medical Science,v. 28, n.3, p. 981-987, 2012
SUSSMAN, C.; BATES-JENSE, B. Wound Care: A collaborative practice manual for health professionals. Philadelphia: Lippincott Williams & Wilkins, 2006
TANIGUCHI, D., DAI, P., HOJO, T., YAMAOKA, Y., KUBO, T., TAKAMATSU, T. . Low-energy laser irradiation promotes synovial fibroblast proliferation by modulating p15 subcellular localization. Lasers Surgery and Medicine, v. 41, n. 3, p. 232-239, 2009
VO-DINH, T. Biomedical Photonics Handbook. In:KARU, T. I. Low-Power Laser Therapy. Florida: CRC Press, 2003