Clinical aspects and detection of Zika virus RNA in several tissues of experimentally infected BALB/cAn mice / Aspectos clínicos e detecção de RNA do vírus Zika em diferentes tecidos de camundongos BALB/cAn infectados experimentalmente

Derick Mendes Bandeira, Gabriela Cardoso Caldas, Fernanda Cunha Jácome, Arthur da Costa Rasinhas, Renata Tourinho Santos, Juliana Fernandes da Silva Amorim, Gisela Freitas Trindade, Debora Ferreira Barreto-Vieira

Abstract


Our group infected BALB/cAn mice with Zika virus to evaluate clinical signs and viral load in several tissues at three different kinetic points. We inoculated fifteen mice with a 100µl of a viral solution to collect nine different tissues, from each animal, for RNA extraction and quantification. Infections caused no lethality. Some of them, however, showed great agitation, hair bristling, and itchy skin. Viral RNA was detected in one sample of heart, eight of the spleen, and two of skeletal muscle. Seven positive detections were from the third day after infection. Only spleen yielded positive results at a later time.

 


Keywords


Zika virus, BALB/cAn mice, qRT-PCR.

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DOI: https://doi.org/10.34119/bjhrv4n1-160

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