Phytochemical Approach and Evaluation of the Osmotic Fragility and Cytotoxic Activity of Piptadenia stipulacea (Benth) Ducke / Abordagem Fitoquímica e Avaliação da Fragilidade Osmótica e Atividade Citotóxica de Piptadenia stipulacea (Benth) Ducke

Renatha Claudia Barros Sobreira, Maria Izabel de Assis Lima, Elizabete Regina Silva Lucena dos Santos, Tainá Maria Santos da Silva, Marcos Aurélio Santos da Costa, Willams Silva da Alves, Roberta M Leite Lima, Sônia Pereira Leite

Abstract


Piptadenia stipulacea (Benth.) Ducke, Fabaceae, is a medicinal plant that can be found in the Caatinga is the only exclusively Brazilian biome. Various biological activities, such as antimicrobial, anti-inflammatory, antifungal and antioxidant activities are reported for this plant. This work investigated the chemical compounds present in the ethanolic extract of the leaves of P. stipulacea (EELPs), being used in the test of osmotic fragility in the blood of sheep and cytotoxic and genotoxic effect in the Allium cepa test with onion roots. Phytochemical analysis were performed by thin-layer chromatography.  Results revealed the presence of tannin, alkaloids, saponins, cumarins, flavonoids and terpenes. The erythrocyte osmotic fragility test showed low hemolysis levels, in the qualitative evaluation of the supernatant and in the result of the hemolytic percentage. Cytological examination with Allium cepa with different concentration EELPs (50 µg.mL-1, 500 µg.mL-1 e 1000 µg.mL-1) no chromosomal abnormality were identified in the cell division process  (Interphase, prophase, metaphase, anaphase, telophase) in relation to the  control group. However, the EELPs at the concentration of 50 µg.mL-1 presented a root development when compared to 500 µg.mL-1 and 1000 µg.mL-1. This demonstrates a pharmacological importance of this plant and low hemolysis index, cell membrane integrity and low toxicity absence of chromosomal abnormality. The results suggest that P. stipulacea may present antigenotoxic.


Keywords


Piptadenia stipulacea, phytochemical, haemolytic action, cytotoxicity, cell division

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DOI: https://doi.org/10.34119/bjhrv3n6-105

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