Effect of Arrabidaea Chica Verlot Hydroalcoholic Extract on Monosodium Iodoacetate-Induced Osteoarthritis of Rat Knees / Efeito do Extrato Hidroalcoólico de Arrabidaea chica Verlot na osteoartrite induzida por Monoiodoacetato de Sódio em joelhos de ratos

Elizabeth Teixeira Noguera Servin, Nicolau Gregori Czeczko, Osvaldo Malafaia, Orlando Jorge Martins Torres, Fernando Cezar Vilhena Moreira Lima, Gyl Eanes Barros Silva, Maria do Socorro de Sousa Cartágenes, João Batista Santos Garcia

Abstract


Introdution: The Arrabidaea chica Verlot (A. chica, ACV), with well-demonstrated anti-inflammatory properties,  appears as an option with therapeutic potential for the osteoarthritis; thus, validating its use is highly relevant. Method: 72 rats were allocated to 3 groups: control, osteoarthritis and phytotherapy {these last two were subjected to osteoarthritis induction, and treated orally with 0.9% normal saline (0.1 mL/100 g) and ACV hydroalcoholic extract (500 mg/kg), respectively, from days 7 to 28}. The 3 groups were subjected to weekly (days 7, 14, 21, 28) assessments including clinical tests (weight-bearing and von Frey), radiological and histopathological analyses.  Fractionation of the ACV´s hydroalcoholic extract was performed and it´s fractions were analysed. Results: The evaluation of the values of the osteoarthritis and phytotherapy groups showed significant difference, with p <0,05 : weight-bearing- on days 14 (29,64 x 35,52), 21 (32,62 x 42,53) and 28 (33,56 x 47,14), von Frey- on days 14 (31,12 x 37,80), 21 (30,24 x 41,48) and 28 (35,78 x 46,09),  x-ray- on days 21 (2,17 x 1,20) and 28 (2,33 x 1,40), and histopathological analysis- on day 28 (0,03 x 2,20). The fractionation of the extract obtained the FH (hexane), FC (chloroform), FAE (ethyl acetate) and FB (butanolic) fractions. The FAE had highest total polypnenolic contents and the FH had the highest concentration of total flavonoids. Conclusion: The ACV extract promoted a reduction in static incapacitance, allodynia, radiological score and degree of synovitis, and FAE and FH fractions are probably the fractions responsible for the anti-inflammatory and analgesic activities of the ACV extract.

Keywords


Osteoarthritis, Knee, Arrabidaea chica Verlot, Rats, Pain.

Full Text:

PDF

References


Haghshenas A, Anand P, Frieri M. Knee osteoarthritis and viscosupplementation: the evolving treatment trend. J Nat Sci 2016; 2: 1-3. http://www.jnsci.org/content/190

WHO (World Health Organization). Obesity and overweight [Internet]. Geneva: WHO [updated 2016 Jun, cited 2016 Nov 17]. Available from: http://www.who.int/mediacentre/factsheets/fs311/en/

Losina E, Paltiel AD, Weinsteins AM, Yelin E, Hunter DJ, Chen SP et al. Lifetime medical costs of knee osteoarthritis management in the United States: Impact of extending indications for total knee arthroplasty. Arthritis Care Res 2015; 67 (2): 203-215. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4422214/pdf/nihms685605.pdf

Chow YY, Chin k. The role of inflammation in the pathogenesis of osteoarthritis. Mediators of Inflammation 2020; 1-19. https://doi.org/10.1155/2020/8293921

Thysen S, Luyten FP, Lories RJU. Targets, models and challenges in osteoarthritis research. Dis Model Mech 2015; 8: 17-30. https://pdfs.semanticscholar.org/1714/1fba833e4b1f46a9b903499a34c29ea431e2.pdf?_ga=2.189710059.1714023472.1588682803-39707615.1585393366

Courties A, Gualillo O, Berenbaum F, Sellam J. Metabolic stress-induced joint inflammation and osteoarthritis. Osteoarthr Cartil 2015; 23: 1955-1965. https://www.oarsijournal.com/article/S1063-4584(15)01173-5/pdf

Gripp, ELP, Carneiro LU, Pereira ISP, Vega MRG,MarinhoBG.Avaliaçãodaspropriedades analgésicas de Anaxagorea dolichocarpa Sprague $ Sandwith LC. Braz J Hea Rev 2020; 3: 382-395. http://www.brazilianjournals.com/index.php/BJHR/article/view/6275/5569

McAlindon TE, Bannuru RR, Sullivan MC, Arden NK, Berenbaum F, Bierma-Zeinstra SM et al. OARSI guidelines for the non-surgical management of knee osteoarthritis. Osteoarthr Cartil2014;22:363388.https://www.oarsi.org/sites/default/files/docs/2014/non_surgical_treatment_of_knee_oa_march_2014.pdf

Karsdal MA, Michaelis M, Ladel C, Siebuhr AS, Bihlet AR, Andersen JR et al. Disease-modifying treatments for osteoarthritis (DMOADs) of the knee and hip: lessons learned from failures and opportunities for the future. Osteoarthr Cartil 2016; 24(12): 2013-2021. https://reader.elsevier.com/reader/sd/pii/S1063458416302072?token=C7427C3DC348A2AB4ABF616492175467FD936DEB68ABC9872C97732A10ABF01ACA7F4DE917A9CD82371760223745525

Sá JC, Almeida-Souza F, Mondêgo-Oliveira R, Oliveira ISS, Lamarck L, Magalhães IFB et al. Leishmanicidal, cytotoxicity and wound healing potential of Arrabidaea chica Verlot. BMCComplMedTherap2016;1:111.https://bmccomplementmedtherapies.biomedcentral.com/track/pdf/10.1186/s12906-015-0973-0

Mafioleti L, Siva Junior IF, Colodel EM, Flach A, Martins DTO. Evaluation of the toxicity and antimicrobial activity of hydroethanolic extract of Arrabidaea chica (Humb. & Bonpl.) B.Verl.JofEthnopharmacol2013;150:576582.http://ri.ufmt.br/bitstream/1/1614/1/DISS_2013_Luciano%20Mafioleti.pdf

Dutra RP, Abreu BVB, Cunha MS, Batista MCA, Torres LMB, Nascimento FRF, Ribeiro MNS, Guerra RNM. Phenolic acids, hydrolyzable tannins, and antioxidant activity of geopropolis from the stingless bee Melipona fasciculata Smith. Journal of Agricultural and Food Chemistry 2014; 62: 2549-2557

Calado GP, Lopes AJO, Costa Junior LM, Lima FCA, Silva LA, Pereira WS, Amaral FMM, Garcia JBS, Cartágenes MSS. Chenopodium ambrosioides L. Reduces Synovial Inflammation and Pain in Experimental Osteoarthritis. PLOS ONE 2015; 10 (11): e0141886. https://doi.org/10.1371/journal.pone.0141886

Morais SV, Czeczko NG, Malafaia O, Ribas Filho JM, Garcia JBS, Miguel MT et al. Osteoarthritis model induced by intra-articular monosodium iodoacetate in rats knee. Acta Cir Bras 2016; 31 (11): 765-773. https://www.scielo.br/pdf/acb/v31n11/1678-2674-acb-31-11-00765.pdf

Kellgren JH and Lawrence JS. Radiological assessment of osteo-arthrosis. Ann Rheum Dis 1957;16:494502.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1006995/pdf/annrheumd00183-0090.pdf

Gerwin N, Bendele AM, Glasson S, Carlson CS. The OARSI histopathology iniciative- recommendations for histological assessments of osteoarthritis in the rat. Osteoarthr Cartil 2010;18:S24S34.https://reader.elsevier.com/reader/sd/pii/S1063458410002475?token=269547892B6BE9E7393ED908C5C31E4DC7C684B6687625046E07C02F045536BDE629EE3937A2392E5F8F00E496E4ED8D

Vasconcelos, CC, Lopes AJO, Sousa ELF, Camelo DS, Lima FCVM, Rocha CQ et al. Effects of extract of Arrabidaea chica Verlot on an experimental model of osteoarthritis. Int J Mol Sci 2019; 20: 4717. file:///Users/elizabethservin/Downloads/ijms-20-04717%20(2).pdf

Gazola AC, Costa GM, Zucolotto SM, Castellanos L, Ramos FA, Lima TCM et al. The sedative activity of flavonoids from Passiflora quadrangularis is mediated throught the GABAergic pathway. Biom Pharmac 2018; 100: 388-393. https://www.ncbi.nlm.nih.gov/pubmed/29454287

Moon S, Jeong J, Jhun JY, Yang EJ, Min J, Choi JY, Cho M. Ursodeoxycholic acid ameliorates pain severity and cartilage degeneration in monosodium iodoacetate-induced osteoarthritis in rats. Imm Netw 2014; 1(14): 45-53. https://synapse.koreamed.org/Synapse/Data/PDFData/0078IN/in-14-45.pdf

Pitcher T, Sousa-Valente J, Malcangio M. The monoiodoacetate model of osteoarthritis pain inthemouse.JVisExp2016;111:16.file:///Users/elizabethservin/Downloads/The_Monoiodoacetate_Model_of_Osteoarthritis_Pain_i.pdf

Arthur K, do Nascimento LC, Figueiredo DADS, de Souza LB, Alfieri FM. Effects of geotherapy and phytotherapy associated with kinesiotherapy in the knee osteoarthritis: randomized double blind study. Acta Fisiátrica 2012; 19: 11-15. file:///Users/elizabethservin/Downloads/geoterapiaefitoterapiaOAdejoelho.pdf

Rezende MUD, Campos GCD. Is osteoarthritis a mechanical or inflammatory disease ? Rev Bras Ortop 2013; 48: 471-474. https://www.scielo.br/pdf/rbort/v48n6/pt_0102-3616-rbort-48-06-00471.pdf

Francisco V, Pino J, Gonzalez-Gay MA, Mera A, Lago F, Gómez R et al. Adipokines and inflammation: is it a question of weight ? Brit J Pharmac 2018; 175: 1569-1579. https://bpspubs.onlinelibrary.wiley.com/doi/epdf/10.1111/bph.14181

Fernihough J, Gentry C, Malcangio M, Fox A, Rediske J, Pellas T, et al. Pain related behaviour in two models of osteoarthritis in the rat knee. Pain 2004; 112: 83-93.

Harvey VL, Dickenson AH. Behavioural and electrophysiological characterization of experimentally induced osteoarthritis and neuropathy in C57B1/6 mice. Mol Pain 2009; 5: 1-11. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2678995/pdf/1744-8069-5-18.pdf

Alles SRA, Smith PA. Etiology and pharmacology of neuropathic pain. Parmacol Rev 2018; (70): 315-347. http://pharmrev.aspetjournals.org/content/pharmrev/70/2/315.full.pdf

Tiulpin A, Thevenot J, Rahtu E, Lehenkari P, Saarakkala S. Automatic knee osteoarthritis diagnosis from plain radiographs: a deep learning-based approach. Scient Reports 2018; 8: 1-10.i https://www.nature.com/articles/s41598-018-20132-7.pdf

Miyamoto S, Nakamura J, Ohtori S, Orita S, Omae T, Nakajima T. et al. Intra-articular injection of mono-iodoacetate induces osteoarthritis of the hip in rats. BMC Musculoskelet Disord2016;17:132138.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4797112/pdf/12891_2016_Article_985.pdf

Scanzello CR, Goldring SR. The role of synovitis in osteoarthritis pathogenesis. Bone 2012; 51: 249-257. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3372675/pdf/nihms-360129.pdf/?tool=EBI

Löfvall H, Newbould H, Karsdal MA, Dziegiel MH, Richter J, Henriksen K et al. Osteoclasts degrade bone an cartilage knee joint compartments through different resorption processes.ArthrResTher2018;(20):67.https://staticcuris.ku.dk/portal/files/214752253/Lofvall_Osteoclasts.pdf

Buckland-Wrignt C. Subchondral bone changes in hand and knee osteoarthritis detected by radiography. Osteoarthr Cartil 2004; 12: S10-S19. https://reader.elsevier.com/reader/sd/pii/S1063458403002498?token=6DA1F0903571BC7B363DEB1D61322BFD41997E0A2EE64C73CD0C02B7CD2659754AD5B7EA23F3F80BE3F1768FCCCBAC22

Zhu S, Zhu J, Zhen G, Hu Y, An S, Li Y et al. Subchondral bone osteoclasts induce sensory innervation and osteoarthritis pain. J Clin Invest 2019; 129 (3): 1076-1093. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6391093/pdf/jci-129-121561.pdf

Michel AF, Melo MM, Campos PP, Oliveira MS, Oliveira FA, Cassali GD. et al. Evaluation of anti-inflammatory, antiangiogenic and antiproliferative activities of Arrabidaea chica crude extracts. J Ethnopharmacol 2015; 165: 29-38.




DOI: https://doi.org/10.34119/bjhrv3n3-236

Refbacks

  • There are currently no refbacks.