Formadores de biofilme de Staphylococcus aureus: sua importância na mastite ovina / Staphylococcus aureus biofilm formators: its importance in ovine mastitis

Autores

  • Gerardo Mancera Cuadros Brazilian Journals Publicações de Periódicos, São José dos Pinhais, Paraná
  • Jorge Pablo Acosta Dibarrat
  • Benjamín Valladares Carranza
  • Carlos Bedolla Cedeño
  • Valente Velázquez Ordoñez

DOI:

https://doi.org/10.34188/bjaerv4n3-125

Palavras-chave:

Staphylococcus aureus, biofilme, importância, mastite, ovelha

Resumo

A mastite é a principal doença que afeta as ovelhas produtoras de leite, embora também seja uma doença recorrente em rebanhos produtores de crias para abastecimento de carne em todo o mundo. Embora existam diferentes patógenos que causam infecção e inflamação da glândula mamária; O Staphylococcus aureus é reconhecido como o principal agente causador da mastite clínica em rebanhos leiteiros, porém também atua concomitantemente com as diferentes espécies de estafilococos coagulase negativos na apresentação da mastite subclínica em ovelhas produtoras de carne. O Staphylococcus aureus contém em sua estrutura genética vários fatores de virulência que desempenham um papel muito importante na patogênese da mastite; um deles é a formação de biofilme que contribui para sua sobrevivência e aderência nas células epiteliais e é de grande importância na disseminação da bactéria no interior de uma glândula mamária afetada. No presente trabalho, uma revisão das infecções causadas por Staphylococcus aureus em ovinos, estafilococos causadores de mastite, fatores de virulência de Staphylococcus aureus, formação de biofilme em cepas de Staphylococcus aureus, sua importância na mastite subclínica e clínica em ovinos e importância na produção de leite de ovelha e resistência a antibióticos devido ao Staphylococcus aureus formador de biofilme.

Referências

Achek R., Hotzel H., Nabi I., Kechida S., Mami D., Didouh N., Tomaso H., Neubauer H., Ehricht R., Monecke S., El?Adawy H. 2020. Phenotypic and Molecular Detection of Biofilm Formation in Staphylococcus aureus Isolated from Different Sources in Algeria. Pathogens 9: 153.

Arsenault J., Dubreuil P., Higgins R., Belanger D. 2008. Risk factors and impacts of clinical and subclinical mastitis in commercial meat-producing sheep flocks in Quebec, Canada. Preventive Veterinary Medicine 87: 373–393.

Azara E., Longheu C., Sanna, G., Tola S. 2017. Biofilm formation and virulence factor analysis of Staphylococcus aureus isolates collected from ovine mastitis. J Appl Microbiol. 123(2):372-379

Caldas A. L. 2015. Baterial biofilms and antibiotic resistence. Revi Cienc Salud 17: 20-27

Camussone C. M. y Calvinho L. F. 2013. Factores de virulencia de Staphylococcus aureus asociados con infecciones mamarias en bovinos: relevancia y rol como agentes inmunógenos. Rev Argent Microbiol. 45(2): 119-130.

Carneiro C. R., Postol E., Nomizo R., Reis L. F. Brentani R. R. 2004 Identification of enolase as a laminin-binding protein on the surface of Staphylococcus aureus. Microbes and Infection 6: 604-608.

Cerca N., Jefferson K. K., Maira-Litrán T., Pier D. B., Kelly-Quintos C., Goldmann D. A., Azeredo J. Pier G. B. 2007 Molecular basis for preferential protective efficacy of antibodies directed to the poorly acetylated form of staphylococcal poly-N-acetyl-beta-(1–6)-glucosamine. Infection and Immunity 75: 3406–3413.

Cheung A. L., Bayer A. S., Zhang G., Gresham H., Xiong Y. Q. 2004. Regulation of virulence determinants in vitro and in vivo in Staphylococcus aureus. FEMS Immunology and Medical Microbiology 40: 1-9

Eisenbeis J., Peisker H., Backes C. S., Bur S., Hölters S., Thewes N., Greiner M., Junker C., Schwarz E. C., Hoth M., Junker K., Preissner K. T., Jacobs K., Herrmann M. Bischoff M. 2017. The extracellular adherence protein (Eap) of Staphylococcus aureus acts as a proliferation and migration repressing factor that alters the cell morphology of keratinocytes. International Journal of Medical Microbiology 307: 116–125.

Foster T. J. 2017. Antibiotic resistance in Staphylococcus aureus. Current status and future prospects. FEMS Microbiology Reviews 41: 430–444.

Fthenakis G. C., Leontides L., Skoufos J., Taitzoglou I. A., Tzora A. 2004. Case report: high prevalence rate of ovine mastitis, caused by coagulase-negative staphylococci and predisposed by increased gossypol consumption. Small Ruminant Research 52: 185–189.

Gelasakis A. I., Mavrogianni, V. S., Petridis, I. G., Vasileiou, N. G. C., Fthenakis, G. C. 2015. Mastitis in sheep: the last 10 years and the future of research. Vet Microbiol 181: 136–146.

Haaber J., Penadés J. R. Ingmer H, 2017 Transfer of antibiotic resistance in Staphylococcus aureus. Trends in Microbiology 25: 893–905.

Hariharan H., Donachie W., Macaldowie C., Keefe G. 2004 Bacteriology and somatic cell counts in milk samples from ewes on a Scottish farm. Can J Vet Res. 68:188–192.

Heying R., van de Gevel J., Que Y. A., Moreillon P., Beekhuizen H. 2007. Fibronectin-binding proteins and clumping factor A in Staphylococcus aureus experimental endocarditis: FnBPA is sufficient to activate human endothelial cells. Thrombosis and Haemostasis 97: 617–626.

Ibberson C. B., Jones C. L., Singh S., Wise M. C., Hart M. E., Zurawski D. V., Horswill A. R. 2014. Staphylococcus aureus hyaluronidase is a CodY-regulated virulence factor. Infection and Immunity 82: 4253–4264.

Kiedrowski ?. R., Crosby H. A., Hernandez F. J., Malone C. L., McNamara J. O., Horswill R. A. 2014. Staphylococcus aureus Nuc2 is a functional, surface-attached extracellular nuclease. PLoS One 9: e95574.

Ko Y. P., Liang X., Smith C. W., Degen J. L., Höök M. 2011. Binding of Efb from Staphylococcus aureus to fibrinogen blocks neutrophil adherence. Journal of Biological Chemistry 286: 9865–9874.

Kobayashi S. D. De Leo F. R. 2013 Staphylococcus aureus protein A promotes immune suppression. M Bio. e00764-13.

Lacey K. A., Leech J. M., Lalor S. J., McCormack N., Geoghegan J. A., McLoughlin R. M. 2017. The Staphylococcus aureus cell wall-anchored protein clumping factor A is an important T cell antigen. Infection and Immunity 85: e00549-17.

Latasa C., Solano C., Penadés J. R. Lasa I. 2006 Biofilm-associated proteins. Comptes Rendus Biologies 329: 849–857.

Loera M. A., Castillo, F. Y. R., Avelar F. J. G., Guerrero A. L. B. 2012. Biopelículas multiespecie: asociarse para sobrevivir. Investigación y Ciencia. 54: 49-56.

Madani A., Garakani K., Mofrad R. K. M. 2017 Molecular mechanics of Staphylococcus aureus adhesin, CNA, and the inhibition of bacterial adhesion by stretching collagen. PLoS One 12: e0179601.

Marogna G., Rolesu S., Lollai S., Tola S., Leori G. 2010. Clinical findings in sheep farms affected by recurrent bacterial mastitis. Small Rumin Res. 88: 119–125.

Mediavilla J. R., Chen L., Mathema B. Kreiswirth B. N. 2012. Global epidemiology of community-associated methicillin resistant Staphylococcus aureus (CA-MRSA). Current Opinion in Microbiology 15: 588–595.

Mørk T., Waage S., Tollersrud S., Kvitle B., Sviland S. 2007. Clinical mastitis in ewes; bacteriology, epidemiology and clinical features. Acta Vet Scand 49: 23–30.

Moroni P., Pisoni G., Varisco G. Boettcher P. 2007 Effect of intramammary infection in Bergamasca meat sheep on milk parameters and lamb growth. J Dairy Res. 74:340–344.

Murray R. J., Pearson J. C., Coombs G. W., Flexman J. P., Golledge C. L., Speers D. J., Dyer J. R., McLellan D. G., Reilly M., Bell J. M., Bowen S.F., Christiansen K. J. 2008. Outbreak of invasive methicillin-resistant Staphylococcus aureus infection associated with acupuncture and joint injection. Infection Control and Hospital Epidemiology 29: 859–865.

Novick. R. P. 2003. Autoinducción y transducción de señales en la regulación de la virulencia estafilocócica. Molecular Microbiology 48: 1429 - 1449.

Otto M. 2013. Staphylococcus aureus toxins. Current Opinion in Microbiology 17, 32-37.

Palmqvist N., Foster T., Tarkowski A. Josefsson E. 2002. Protein A is a virulence factor in Staphylococcus aureus arthritis and septic death. Microbial Pathogenesis. 33: 239–249.

Penesyan A., Gillings M., Paulsen l. T. 2015. Antibiotic Discovery: Combatting Bacterial Resistance in Cells and in Biofilm Communities. Molecules. 20: 5286-5298

Petridis I.G., Fthenakis G.C. 2014. Administration of antibiotics to ewes at the beginning of the dry-period. J. Dairy Res.; 81: 9-15

Schönborn S., Wente N., Paduch J. H., Krömker V. 2017. In vitro ability of mastitis causing pathogens to form biofilms. Journal of Dairy Research 84: 198–201.

Selvaggi M., D’ Alessandro A. G., Dario C. (2017) Environmental and genetic factors affecting milk yield and quality in three Italian sheep breeds. Journal of Dairy Research 84, 27–31.

Spaan N. A., Van Strijp J. A. G., Torres V. J., 2017. Leukocidins: staphylococcal bi-component pore-forming toxins find their receptors. Nature Reviews Microbiology 15: 435–447.

Stutz K., Stephan R. Tasara T. 2011. Spa, ClfA, and FnbA genetic variations lead to Staphaurex test-negative phenotypes in bovine mastitis Staphylococcus aureus isolates. Journal of Clinical Microbiology 49, 638–646.

Tam K., Torres V. J. 2019. Staphylococcus aureus secreted toxins and extracellular enzymes. Microbiol. Spectr. 7: doi:10.1128/microbiolspec.GPP3?0039?2018

Tel O. Y., Aslantas O., Keskin O., Yilmaz E. S., Demir, C. 2012. Investigation of the antibiotic resistance and biofilm formation of Staphylococcus aureus strains isolated from gangrenous mastitis of ewes. Acta Vet Hung. 60: 189–197.

Vasileiou N. G. C., Chatzopoulos D. C., Gougoulis D. A., Sarrou S., Katsafadou A. I., Spyrou V., Mavrogianni V. S., Petinaki E., Fthenakis G. C. 2018a. Slime-producing staphylococci as causal agents of subclinical mastitis in sheep. Veterinary Microbiology 224: 93–99.

Vasileiou N. G. C., Cripps P. J., Ioannidi K. S., Chatzopoulos D. C., Gougoulis D. A., Sarrou S., Orfanou D. C., Politis A.P., Calvo González-Valerio T., Argyros S., Mavrogianni V. S., Petinaki E., Fthenakis G. C. 2018b. Extensive countrywide field investigation of subclinical mastitis in sheep in Greece. J. Dairy Sci 10: 7297-7310.

Vasileiou N. G., Chatzopoulos C., D. C., Cripps P. J., Ioannidi K. S., Gougoulis D. A., Chouzouris T. M., Lianou D. T., Calvo Gonzalez-Valerio T., Guix Vallverdu R., Argyros S., Cesio M., Font I., Mavrogianni V. S., Petinaki E., Fthenakis G. C. 2019. Evaluation of efficacy of a biofilm-embedded bacteria-based vaccine against staphylococcal mastitis in sheep a randomized, placebo controlled field study. J. Dairy Sci. 102:9328–9344.

Wang B. y Muir T. W., 2016. Regulation of virulence in Staphylococcus aureus: molecular mechanisms and remaining puzzles. Cell Chemical Biology 23: 214–224.

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Publicado

2021-09-08

Como Citar

Cuadros, G. M., Dibarrat, J. P. A., Carranza, B. V., Cedeño, C. B., & Ordoñez, V. V. (2021). Formadores de biofilme de Staphylococcus aureus: sua importância na mastite ovina / Staphylococcus aureus biofilm formators: its importance in ovine mastitis. Brazilian Journal of Animal and Environmental Research, 4(3), 4408–4423. https://doi.org/10.34188/bjaerv4n3-125

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