Avaliação da proliferação celular de clostridium perfringens em intestino delgado de ruminantes / Evaluation of cellular proliferation of clostridium perfringens in small intestine of ruminants

Simone Miyashiro, Alessandra Figueiredo de Castro Nassar, Renata Haddad Esper, Nilson Roberti Benites

Resumo


This study evaluated the isolation, molecular typing and celular proliferation of C. perfringens in small intestine of cattle and sheep distributed in case and control groups. In the case group, out of the 61 bovine samples analyzed, C. perfringens was isolated in 29 (47.54%) and in 4 (33.34%) from 12 sheep samples. In the control group, we did not isolate the microorganism in the bovine samples (73 samples), however it was possible in 5 (20.83%) out of the 24 sheep samples.  All isolates (100%) were classified as type A, and cell quantification showed that in control group, all cattle samples resulted in <10 CFU/g of intestinal content while in the study group presented a median value of 104 CFU/g, varying from <10 CFU/g through 108 CFU/g. In ovine group there was a clear divison of values between the two groups. Molecular typing corroborates with other studies regardin the epidemiological importance of type A in enterotoxaemia in ruminants, thus cellular quantification data allow us to conclude that healthy animals present a basal level of C. perfringens lower than 10 CFU/g that does not allow its isolation.


Palavras-chave


quantification, enterotoxemia, clostridiosis, cattle, sheep.

Texto completo:

PDF

Referências


Hatheway CL. Toxigenic clostridia. Clin Microbiol Rev. 1990, v. 3, n. 1, p.66-98.

Niilo L. Clostridium perfringens in animal disease: a review of current knowledge. Can Vet J. 1980, v. 21, n.5, p.141-8.

Kriek NPJ, Odendaal MW, Hunter P. Clostridium perfringens type D enterotoxaemia. In: Coetzer J.A.W.; Thomson G.R ; Tustin R.C (Eds.). Infectious diseases of livestock with special reference to Southern Africa. Oxford: Oxford University. 1994, p.1315-1322.

Popoff MR. Les entérotoxémies. Revue Médecine Vétérinaire. 1989, v.140,n. 6, p. 479-491.

Songer JG. Clostridial enteric diseases of domestic animals. Clin Microbiol Rev. 1996, v.9, n.2, p.216-34.

MAPA 2003. Contagem de Clostridium sulfite redutores e de Clostridium perfringens. Instrução normativa 62 de 26/08/2003.

Baums CG, Schotte U, Amtsberg G, Goethe R. Diagnostic multiplex PCR for toxin genotyping of Clostridium perfringens isolates. Veterinary Microbiology. 2004, v.100, p.11-16.

Meer RR, Songer JG. Multiplex PCR method for genotyping Clostridium perfringens. American Journal of Veterinary Research. 1997, v. 58, p.702-705.




DOI: https://doi.org/10.34188/bjaerv4n3-092

Apontamentos

  • Não há apontamentos.