First occurrence of dsRNA in Rhizoctonia solani AG2-2 LP from turfgrass Zoysia japonica in Brazil / Primeiro relato de RNA dupla fita em Rhizoctonia solani AG2-2 LP, patógeno de Zoysia japônica no Brasil

Autores

  • Maria Aurea Saboya Chiaradia Picarelli Brazilian Journals Publicações de Periódicos, São José dos Pinhais, Paraná
  • Danielle Gobatto
  • Eliana Borges Rivas
  • Addolorata Colariccio

DOI:

https://doi.org/10.34188/bjaerv4n1-097

Palavras-chave:

mycovirus, dsRNA, Rhizoctonia solani AG 2-2 LP

Resumo

Rhizoctonia solani, a complex species comprising groups and sub-groups of somatic compatibility (anastomosis groups – AG), is a cosmopolitan pathogenic fungus that causes important diseases in a wild range of plants. One example is R. solani AG2-2 LP, the causal agent of large patch disease of C4 grasses, primarily zoysiagrass. The occurrence of this pathogen in Brazil causes a severe disease that is difficult to control. Mycoviruses, viruses that replicate in fungi, are ubiquitous. Since their first report in the 1960s, they have been searched for in many classes of fungi with the purpose of finding new tools to manage diseases, improve crops and address the challenges of climate change. The genome of mycoviruses mostly consists of double-stranded RNA (dsRNA) and they may or may not induce symptoms in their host. dsRNAs have already been reported in species of Rhizoctonia, in some cases reducing virulence. We searched for dsRNAs in 12 Brazilian isolates of R. solani AG 2-2 LP, a pathogen of zoysiagrass. All isolates showed dsRNAs were present in a similar profile to each other, irrespective of the virulence of those isolates on zoysiagrass. Among the isolates the number of dsRNA segments ranged from three to eight, with different sizes, never smaller than 2 kb and with segments greater than 10 Kb in all isolates. Detection of dsRNA in all R. solani isolates may be indicative of mycoviruses being a frequent presence in these fungi.

Referências

Smiley RW, Dernoeden PH, Clarke BB (2007) Rhizoctonia Diseases. In Smiley RW, Dernoeden PH, Clarke BB (eds) Compendium of Turfgrass Diseases, 3rd edn. APS Press, St. Paul pp 78-85

Sampaio HA (2012) Manutenção em gramados ornamentais. In: Backes C, Godoy LJG, Mateus CMDA, Santos AJM, VillasBoas RL, Oliveira MR (eds) Tópicos atuais em gramados III, VI SIGRA – Simpósio sobre Gramados. FCA/UNESP. Botucatu, SP, pp192-200. http://infograma.com.br/wp-content/uploads/2015/11/topico3.pdf Accessed 08 November 2019

Zanon ME (2015). Desenvolvimento de grama ‘Esmeralda’, grama bermudas ‘Tifway 410’ e ‘Celebration’ submetidas a aplicação de reguladores de crescimento. Ph.D. thesis, Universidade Estadual Paulista/UNESP, Jaboticabal. http://repositorio.unesp.br/bitstream/handle/11449/136722/000859912.pdf?sequence=1 Accessed 08 November 2019

Das S, Falloon RE, Stewart A, Pitman AR (2014) Molecular characterization of an endornavirus from Rhizoctonia solani AG-3 PT infecting potato. Fungal Biology 118:924-934. http://doi.org/10.1016/j.funbio.2014.08.003

Castanho B, Butler EE, Shepard R (1978) The association of double strain RNA with Rhizoctonia decline. Phytopathology 68:1515-1519. http://doi.org/10.1094/phyto-68-1515

Jian J, Lakshman DK, Tavantzis SM (1997) Association of distinct double-stranded RNAs with enhanced or diminished virulence in Rhizoctonia solani infecting potato. Molecular Plant-Microbe Interactions 10:1002-1009. http://doi.org/10.1094/MPMI.1997.10.8.1002

Zheng L, Zhang M, Chen Q, Zhu M, Zhou E (2014). A novel mycovirus closely related to viruses in the genus Alphapartitivirus confers hypovirulence in the phytopathogenic fungus Rhizoctonia solani. Virology 456/457: 220-226. http://doi.org/10.1016/j.virol.2014.03.029

Nuss DL (2005) Hypovirulence: Mycoviruses at the fungal-plant interface. Nature Reviews Microbiology 3: 632-642. http://doi.org/10.1038/nrmicro1206

Ghabrial SA, Suzuky N (2009) Viruses of plant pathogenic fungi. Annual Review of Phytopathology 47:353-384. http://doi.org/10.1146/annurev-phyto-080508-081932

Milgroom MG, Hillman BI (2011) The ecology and evolution of fungal viruses. In: Hurst DJ (ed) Studies in viral ecology: microbial and botanical host systems. 1 st edn. John Wiley & Sons, Hoboken pp 217-253.

Yu X, Li B, Fu Y, Xie J, Cheng J, Ghabrial SA, Li G, Yi X, Jiang D (2010) A geminivirus-related DNA mycovirus that confers hypovirulence to a plant pathogenic fungus. Proceedings of the National Academy of Sciences of the Unites States of America 107:8387-8392. http://doi.org/10.1073/pnas.0913535107

Liu L, Xie J, Cheng J, Fu Y, Li G, Yi X (2014) Fungal negative-stranded RNA virus that is related to bornaviruses and nyaviruses. Proceedings of the National Academy of Sciences of the Unites States of America 111:12205-12210. http://doi.org/10.1073/pnas.1401786111

Picarelli MASC, Gobatto D, Patricio FRA, Rivas EB, Colariccio A (2017) Virus que infectam fungos fitopatogênicos. Arquivos do Instituto Biológico 84:1-15. http://doi.org/10.1590/1808-1657000162016

Picarelli MASC, Patricio FRA, Harakava R, Rivas EB, Colariccio A (2020) First report of Rhizoctonia solani Kühn AG 2-2 LP in Zoyzia japonica Steud in Brazil. Summa Phyatopathologica v.46, n.4. http://doi.org/10.1590/0100-5405/221916

Morris TJ, Dodds JA (1979) Isolation and analysis of double-stranded RNA from virusinfected plant and fungal tissue. Phytopathology 69: 854-858. http://doi.org/10.1094/Phyto-69-854 Accessed 08 November 2019

Gobatto D, Chaves ALR, Harakava R, Marque JM, Daròs JÁ, Eiras M (2014) Crhysantemum Stunt Viroid in Brazil: survey, identification, biological and molecular characterization and detection methods. Journal of Plant Pathology 96:1-9. http://doi.org/10.4454/JPP.V96I1.037

ICTV Masters Spicies List 2018b. v2https://talk.ictvonline.org/files/master-species-lists/m/msl/8266. Accessed 05 December 2019

Tavantzis SM, Lakshman DK, Liu C (2002) Double-Stranded RNA elements modulating virulence in Rhizoctonia solani. In: Tavantzis SM (ed) dsRNA Elements - Concepts and Applications in agriculture, forestry and medicine. CRC Press, Boca Raton, FL, 2002, pp 191-211

Picarelli MASC, Forgia M, Rivas EB, Nerva L, Chiapello M, Turina M, Colariccio A (2019) Extreme Diversity of Mycoviruses Present in Isolates of Rhizoctonia solani AG2-2 LP From Zoysia japonica From Brazil. Frontiers in Cellular and Infection Microbiology 9: 244. http://doi.org/10.3389/fcimb.2019.00244

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Publicado

2021-03-08

Como Citar

Picarelli, M. A. S. C., Gobatto, D., Rivas, E. B., & Colariccio, A. (2021). First occurrence of dsRNA in Rhizoctonia solani AG2-2 LP from turfgrass Zoysia japonica in Brazil / Primeiro relato de RNA dupla fita em Rhizoctonia solani AG2-2 LP, patógeno de Zoysia japônica no Brasil. Brazilian Journal of Animal and Environmental Research, 4(1), 1211–1218. https://doi.org/10.34188/bjaerv4n1-097

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