Acute Toxicity of Cigarette Butts Leachate on Nauplii of Artemia sp. / Toxicidade aguda do lixiviado de bitucas de cigarro sobre Náuplios de Artemia sp.

Denis Moledo de Souza Abessa, Andressa dos Santos Barbosa Ortega, Gabriela Pustiglione Marinsek, Larissa Yoshida Roselli, Luiza De David Chelotti, Fernando Cesar Perina


Cigarette butts (CBs) are composed by synthetic polymers and products from the combustion of tobacco. They are improperly discarded in large quantities in the environment, representing a major litter type impacting the coastal ecosystems. CBs contain a large number of hazardous chemical substances and represent potential threats to marine organisms. This investigation assessed the acute toxicity of CBs leachates on the microcrustacean Artemia sp., considering lethal effects and morphometric analysis. A leachate solution was obtained by the addition of 5 smoked CBs in 500 ml filtered seawater, and agitation for 3 days. Nauplii of Artemia sp. were obtained from cists and exposed to different concentrations of the leachate (100, 50, 25, 12.5 and 6.25 %). After 48 h, the mortalities were recorded and the survivors were analyzed for their size and presence of morphological deformities. The results showed significant differences from the concentration of 50 % (LOEC) and the lethal concentration to 50% organisms (LC50-48h) was 45.3 (39.3-52.1) %. No differences were observed regarding the body size, but morphological deformities were more frequent in the organisms exposed to the highest concentrations. The presence of CBs in the environment may threaten the marine species, because the leachate is toxic.


Microcrustacean, mortality, cigarette butts, marine pollution, marine litter.

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