Interactions of Bacillus thuringiensis and organic biofertilizer as elicitors in Mikania laevigata and phytopathogenic action / Interações de Bacillus thuringiensis e biofertilizante orgânico como elicitores em Mikania laevigata e ação fitopatogênica

Wellington Soares, Diouneia Lisiane Berlitz, Sarazete Izidia Vaz Pereira, Lidia Mariana Fiuza

Resumo


To increase coumarin production in medicinal plants, this research evaluated the elicitor effect of bacterial products (Dipel® and Xentari®) and biofertilizer (Vairo) on bioactive production when applied to Mikania laevigata. To estimate the induction of antifungal substances, aqueous extract of M. laevigata leaves was used against Fusarium oxysporium and Sclerotinia sclerotiorum. In the trials, clones were prepared from a matrix plant, where 22mL of treatments were sprayed on each plant, totaling 288 plants. There were 3 repetitions of 3 plants and 8 treatments. The leaves were dehydrated for 48h at 45ºC and quantification of coumarin was performed in High Performance Liquid Chromatography. In the fungal assays, CFUs and conidiogenesis were evaluated. Data revealed 85% germination in M. laevigata clones. The coumarin quantification curve resulted in the linear equation: y = 9E, 7x + 914140, and the coefficient of determination was R2 = 0.9893. The results show that the mixture of Vairo and Xentari® act as coumarin elicitors in M. laevigata, being recommended to collect the leaves between 2 and 7 days. Plant protection against F. oxysporium was also observed by inhibiting conidiogenesis.


Palavras-chave


plant elicitation, Bacillus thuringiensis, Fusarium sp., Sclerotinia sp., Mikania laevigata.

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Referências


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DOI: https://doi.org/10.34188/bjaerv4n1-033

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