Draft genome sequence of Bacillus thuringiensis strain s1785, an isolate showing insecticidal activity / Sequência do genoma de Bacillus thuringiensis strain s1785, um isolado que mostra actividade insecticida

Authors

  • Érica Martins
  • Paulo Queiroz
  • Priscila Grynberg
  • Roberto Togawa
  • Rose Monnerat

DOI:

https://doi.org/10.34115/basrv6n3-020

Keywords:

ngs, plant promotion growth, cyt toxin, diptera.

Abstract

Bacillus thuringiensis is a Gram-positive bacterium which produces insecticidal proteins effective to control several medical and agriculture insect pests. These proteins belong to the Cry, Cyt, Vip and Sip families. This work describes the draft genome sequence of B. thuringiensis S1785, which contains genes encoding the parasporal crystal cry, like Cry4Aa, Cry4Ba, Cry10Aa, Cry11Aa, Cry60Aa and Cry60Ba and Cyt genes, Mpp60Aa and Mpp60Ba. The gene Spp1Aa was identified in the S1785 genome. Genes coding for plant growth promotion pathway were identified.

References

He J, Wang J, Yin W, Shao X, Zheng H, Li M, Zhao Y, Sun M, Wang S, Yu Z. 2011. Complete Genome Sequence of Bacillus thuringiensis subsp. chinensis Strain CT-43. Journal of Bacteriology. 193:3407–3408. doi: 10.1128/JB.05085-11.

Porcar M, Navarro L, Jimenez-Peydro R, 2006. Pathogenicity of intra-thoracically administered Bacillus thuringiensis spores in Blatta orientalis. J Invertebr Pathol. 93:63–66. doi: 10.1016/j.jip.2006.05.001.

- Kearse M, Moir R, Wilson A, Stones-Havas S, Cheung M, Sturrock S, Buxton S, Cooper A, Markowitz S, Duran C, Thierer T, Ashton B, Meintjes P, Drummond A. (2012). Geneious Basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics. 28:1647-1649. doi: 10.1093/bioinformatics/bts199.

- Delcher AL, Kasif S, Fleischmann RD, Peterson J, White O, Salzberg SL. 1999. Alignment of whole genomes. Nucleic Acids Res. 27:2369–2376. doi: 10.1093/nar/27.11.2369.

– Schattner P, Brooks AN, Lowe TM. 2005. The tRNAscan-SE, snoscan and snoGPS web servers for the detection of tRNAs and snoRNAs. Nucleic Acids Res. 33:W686-W689. doi: 10.1093/nar/gki366.

- Lagesen K, Hallin P, Rodland EA, Staerfeldt HH, Rognes T, Ussery DW. 2007. RNAmmer: consistent and rapid annotation of ribosomal RNA genes. Nucleic Acids Res. 35:3100–3108. doi: 10.1093/nar/gkm160.

- Boeckmann B, Bairoch A, Apweiler R, Blatter MC, Estreicher A, Gasteiger E, Martin MJ, Michoud K, O’Donovan C, Phan I, Pilbout S, Schneider M. 2003. The SWISS-PROT protein knowledgebase and its supplement TrEMBL in 2003. Nucleic Acids Res. 31:365–370. http:// dx.doi.org/10.1093/nar/gkg095. http:// dx.doi.org/10.1093/nar/gkg095.

- Tatusov RL, Natale DA, Garkavtsev IV, Tatusova TA, Shankavaram UT, Rao BS, Kiryutin B, Galperin MY, Fedorova ND, Koonin EV. 2001. The COG database: new developments in phylogenetic classification of proteins from complete genomes. Nucleic Acids Res. 29:22–28. http:// dx.doi.org/10.1093/nar/29.4.e22. http:// dx.doi.org/10.1093/nar/29.4.e22.

- Kanehisa M, Araki M, Goto S, Hattori M, Hirakawa M, Itoh M, Katayama T, Kawashima S, Okuda S, Tokimatsu T, Yamanishi Y. 2008. KEGG for linking genomes to life and the environment. Nucleic Acids Res. 36:D480–D484. http://dx.doi.org/10.1093/nar/gkm882.

Published

2022-05-09

How to Cite

Martins, Érica, Queiroz, P., Grynberg, P., Togawa, R., & Monnerat, R. (2022). Draft genome sequence of Bacillus thuringiensis strain s1785, an isolate showing insecticidal activity / Sequência do genoma de Bacillus thuringiensis strain s1785, um isolado que mostra actividade insecticida. Brazilian Applied Science Review, 6(3), 1037–1040. https://doi.org/10.34115/basrv6n3-020

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Section

Original articles