Evaluation of celery extract (Apium greveolens L.) as a natural curing agent in the production of Italian-type Salami with native starter cultures / Avaliação do extrato de aipo (Apium greveolens L.) como agente de cura natural na produção de salame do tipo italiano com culturas iniciadoras nativas

Ana Rita Carboni Ritter, Graciele Daiana Funck, Luciana Prietto, Guilherme da Silva Dannenberg, Juliana de Lima Marques, Claudio Eduardo dos Santos Cruxen, Wladimir Padilha da Silva, Ângela Maria Fiorentini


The aim of this study was to evaluate celery extract as a natural curing agent in the production and characterization of Italian-type Salami with native starter cultures SxLp (S. xylosus AD1 and L. plantarum AJ2). Two formulations were produced for Italian-type Salami, to one of which was added 1.4% celery extract (T1) and to the other 0.3% commercial curing salt (T2). Maintaining the same conditions of temperature, relative humidity and air velocity, the salamis were matured for 26 days, and physicochemical, microbiological and sensorial analyses were performed. Starter culture SxLp produced a reduction in pH, promoting greater acidification of salami and reduction of nitrate, allowing color formation in the product. Moreover, the cultures remained viable (> 7 log CFU.g-1), in both treatments, during the 26 days of maturation. The combination of native starter culture with celery extract promoted microbiological safety, adequate texture and the characteristic color of cured meat sausage. Salami T1, containing starter cultures SxLp and celery extract, was well accepted by the judges, most of whom would often buy the product. Thus, the application of celery extract as a curing agent together with the addition of the native starter cultures, proved to be viable for production of Italian-type salami.



lactic acid bacteria, fermented sausage, coagulase negative staphylococci, vegetable extract, sodium nitrate.

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Association of Official Analytical Chemists - AOAC (2012) Official methods of analysis, 19th ed. Maryland

American Public Health Association - APHA (2002) Compendium of methods for the microbiological examination of foods. Washington.

Arnau J, Serra X, Comaposada J, et al. (2007) Technologies to shorten the drying period of dry-cured meat products. Meat Sci 77:81–89. doi: 10.1016/j.meatsci.2007.03.015

Barrière C, Centeno D, Lebert A, et al. (2001) Roles of superoxide dismutase and catalase of Staphylococcus xylosus in the inhibition of linoleic acid oxidation. FEMS Microbiol Lett 201:181–185. doi: 10.1016/S0378-1097(01)00271-3

Benito, M. A. J., Rodrı́guez, M., Martı́n, A., Aranda, E., & Córdoba, J. J. (2004). Effect of the fungal protease EPg222 on the sensory characteristics of dry fermented sausage“salchichón” ripened with commercial starter cultures. Meat Science, 67(3), 497-505.

Bertol TM, Fiorentini AM, José M, et al. (2012) Rosemary extract and celery-based products used as natural quality enhancers for colonial type salami with different ripening times. Revista Ciência e Tecnologia de Alimentos, 32:783–792.

Brasil. Ministério da Agricultura Pecuária e Abastecimento. (1999) Métodos Analíticos Físico-Químicos, para Controle de Produtos Cárneos e seus Ingredientes - Sal e Salmoura. Brasil

Brasil. Ministério da Agricultura Pecuária e Abastecimento. (2000) Instrução normativa n.o 22, de 31 de julho de 2000. Regulamento técnico de identidade e qualidade do salame tipo italiano. doi: 10.1073/pnas.0703993104

Brasil. Ministério da Saúde. (2001) Resolução RDC no 12, de 02 de janeiro de 2001. Dispões dobre o Regulamento Técnico Sobre Padrões Microbiológicos Para Alimentos. Diário Oficial [da] União da República Federativa do Brasil, Brasília, DF, 2001.

Brasil. Ministério da Saúde. (2019) Resolução RDC nº 272, de 14 de março de 2019. Estabelece os aditivos alimentares autorizados para uso em carnes e produtos cárneos. Brasil, DF.

CDC. C for DC and P (2007) Botulism associated with canned chili sauce. http://www.cdc.gov/botulism/botulism.htm. Accessed 27 Jan 2016.Chen, Q., Kong, B., Han, Q., Xia, X., & Xu, L. (2017). The role of bacterial fermentation in lipolysis and lipid oxidation in Harbin dry sausages and its flavour development. LWT - Food Science and Technology, 77, 389–396. https://doi.org/10.1016/j.lwt.2016.11.

Chen, Q., Liu, Q., Sun, Q., Kong, B., & Xiong, Y. (2015). Flavour formation from hydrolysis

of pork sarcoplasmic protein extract by a unique LAB culture isolated from Harbin dry sausage. Meat Science, 100, 110–117. https://doi.org/10.1016/j.meatsci. 2014.10.001.

Choi, Y. S., Kim, T. K., Jeon, K. H., Park, J. D., Kim, H. W., Hwang, K. E., Kim, Y. B. (2017). Effects of Pre-Converted Nitrite from Red Beet and Ascorbic Acid on Quality Characteristics in Meat Emulsions. Korean Journal for Food Science and Animal Resources, 37, 288-296.

Choi YS, Choi JH, Kim HY, et al. (2011). Effect of lotus (Nelumbo nucifera) leaf powder on the quality characteristics of chicken patties in refrigerated storage. Korean J Food Sci Anim Resour ;31:9-18.

Cruxen, C. E. D. S., Braun, C. L. K., Fagundes, M. B., Gularte, M. A., Wagner, R., Padilha da Silva, W., & Fiorentini, Â. M. (2018). Development of fermented sausage produced with mutton and native starter cultures. Lwt, 95(April), 23–31. http://doi.org/10.1016/j.lwt.2018.04.060

Cruxen, C. E. D. S., Funck, G. D., Dannenberg, G. D. S., Haubert, L., Marques, J. D. L., Kroning, I. S., … Fiorentini, Â. M. (2017). Characterization of Staphylococcus xylosus LQ3 and its application in dried cured sausage. LWT - Food Science and Technology, 86, 538–543.

Cruxen, C.E.S.; Funck, G.D.; Haubert, L.; Dannenberg, G.S.; Marques, J.L.; Chaves, F.C.; Silva, W.P.; Fiorentini, A.M. (2019) Selection of native bacterial starter culture in the production of fermented meat sausages: Application potential, safety aspects, and emerging technologies. Food Research International, v. 122, p. 371-382.

Da Costa, W. K. A., de Souza, G. T., Brandão, L. R., de Lima, R. C., Garcia, E. F., dos Santos Lima, M., … Magnani, M. (2018). Exploiting antagonistic activity of fruit-derived Lactobacillus to control pathogenic bacteria in fresh cheese and chicken meat. Food Research International, 108, 172–182. http://doi.org/10.1016/j.foodres.2018.03.045

Essid I, Ben Ismail H, Bel Hadj Ahmed S, et al. (2007) Characterization and technological properties of Staphylococcus xylosus strains isolated from a Tunisian traditional salted meat. Meat Sci 77:204–212. doi: 10.1016/j.meatsci.2007.03.003

Fabbri A, Cevoli C, Silaghi FA, Guarnieri A (2011) Numerical Simulation of Physical Systems in Agri-Food Engineering. Jounal Agric Engeneering 4:1–7.

Fiorentini AM, Sawitzki MC, Bertol TM, et al. (2009) Phenotypic and molecular characterization of Staphylococcus xylosus: Technological potential for use in fermented sausage. Brazilian Arch Biol Technol 52:737–746. doi: 10.1590/S1516-89132009000300026

Georgiev, V.G., Weber, J., Kneschke, E.M., NedyalkovDenev, P., Bley, T., Pavlov, A.I. Antioxidant activity and phenolic content of betalain extracts from intact plants and hairy root cultures of the red beetroot Beta vulgaris cv.detroit dark red. Plant Foods for Human Nutrition, 65 (2010), pp. 105-111

ISO. (2014). ISO 11136. Sensory analysis: Methodology-General guidance for conducting hedonic tests with consumers in a controlled area (:2014). Geneva, Switzerland: International Organization for Standardization.

Jin, S-K., Choi J. S., Yang, H-S., Park, T-S., Yim, D-G. (2018) Natural curing agents as nitrite alternatives and their effects on the physicochemical, microbiological properties and sensory evaluation of sausages during storage. Meat Science, v.146, p.34-40.

Keeton, J. T., Osburn, W. N. Hardin, M. D., Bryan, N. S. & Longnecker, M. T. (2012). A national survey of the nitrite/nitrate concentrations in cured meat products and nonmeat foods available at retail. Journal of Agricultural and Food Chemistry, 60, 3981-3990.

Kerry J, Kerry J, Ledward D (2002) Meat Processing. Improving Quality. Woodhead Publishing Limited, Cambridge

Landeta, G., Curiel, J. a., Carrascosa, a. V., Muñoz, R., & De las Rivas, B. (2013). Technological and safety properties of lactic acid bacteria isolated from Spanish dry-cured sausages. Meat Science, 95(2), 272–280. http://doi.org/10.1016/j.meatsci.2013.05.019

Leroy, F., & De Vuyst, L. (2004). Lactic acid bacteria as functional starter cultures for the food fermentation industry. Trends in Food Science & Technology, 15(2), 67–78. http://doi.org/10.1016/j.tifs.2003.09.004

Leroy F, Verluyten J, De Vuyst L (2006) Functional meat starter cultures for improved sausage fermentation. Int J Food Microbiol 106:270–285. doi: 10.1016/j.ijfoodmicro.2005.06.027

Parthasarathy DK, Bryan NS (2012) Sodium nitrite: The “cure” for nitric oxide insufficiency. Meat Sci 92:274–279. doi: 10.1016/j.meatsci.2012.03.001

Pinto MF, Ponsano EHG, Heinemann RJB (2001) Bactérias envolvidas no processamento de produtos cárneos – uma revisão. Bol do BCTA 35:109–116.

Raharjo S, Sofos JN, Schmidt GR (1992) Improved speed, specificity, and limit of determination of an aqueous acid extraction thiobarbituric acid-C18 method for measuring lipid peroxidation in beef. J Agric … 40:2182–2185. doi: 0021-8561 1921 1440-21 82$03.00/0

Ramos, E. M., & Gomide, L. A. M. (2007). Avaliação da qualidade de carnes: fundamentos e metodologias. Viçosa: Ed. da UFV, 599 p

Riazi, F., Zeynail, F., Hoseini, E., & Behmadi, H. (2016). Effect of dry red grape pomace as a nitrite substitute on the microbiological and physicochemical properties and residual nitrite of dry cured sausage. Nutrition and Food Sciences Research, 3, 37-44.

Sawitzki MC, Fiorentini AM, Brod FC a, et al. (2007) Phenotypic characterization and species-specific PCR of promising starter culture strains of Lactobacillus plantarum isolated from naturally fermented sausages. Brazilian J Microbiol 38:547–552. doi: 10.1590/S1517-83822007000300031

Schilling, M.W., Pham, A.J., Williams, J.B., Xiong, Y.L., Dhowlaghar, N., Tolentino, A.C., Kin, S. (2018) Changes in the physiochemical, microbial, and sensory characteristics of fresh pork sausage containing rosemary and green tea extracts during retail display. Meat Science, v. 143, p. 199-209.

Sebranek JG, Bacus JN (2007) Cured meat products without direct addition of nitrate or nitrite: what are the issues? Meat Sci 77:136–147. doi: 10.1016/j.meatsci.2007.03.025

Shimokomaki M, Olivo R, Terra NN, Franco BDGM (2006) Atualidades em Ciência e Tecnologia de Carnes. Livraria Varela, São Paulo, 236 p.

Skibsted, L.H. (2011). Nitric oxide and quality and safety of muscle based foods. Nitric oxide. 24, 176-183.

Song, E-Y. S., Pyun, C-W., Hong, G-E., Lim, K-W., Lee, C-H. (2014) Effect of Addition of Allium hookeri on the Quality of Fermented Sausage with Meat from Sulfur Fed Pigs during Ripening. Korean Journal Food Science of Animal Resources. v. 34, n. 3, pp. 263-272.

Talon, R., Walter, D., Chartier, S., Barrière, C., & Montel, M. C. (1999). Effect of nitrate and incubation conditions on the production of catalase and nitrate reductase by staphylococci. International Journal of Food Microbiology, 52(1-2), 47–56. http://doi.org/10.1016/S0168-1605(99)00127-0

Wang, Q., Wang, J., Ding, W., Zhang, D., Reed, K., Zhang, B. (2018) Alternatives to carcinogenic preservatives in Chinese Sausage - Sorbic acid-loaded chitosan/tripolyphosphate nanoparticles. International Journal of Biological Macromolecules, v. 120, p. 28-33.

Yildz-Turp G, Serdaroglu M (2010) Effects of using plum puree on some properties of low fat beef patties. Meat Sci 86:896–900. doi: 10.1016/j.meatsci.2010.07.009

DOI: https://doi.org/10.34117/bjdv6n5-137


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