Pancreas glucagon immunolocalization in the scorpion mud turtle, kinosternon scorpioides Running title: pancreas glucagon immunolocalization in turtle

José Gomes Pereira, Mayra da Silva Oliveira, Diego Carvalho Viana, Ana Lúcia Abreu-Silva, Ferdinan Almeida Melo


Jurará (Kinosternon scorpioides) is a semi-aquatic fresh water turtle. It is a species of the Brazilian fauna in imminent risk of extinction. Thus studies have been carried out to develop its rearing in captivity. In the present study pancreas fragments were used from six adult K. Scorpioides specimens (three males and three females) blocked in paraffin. Semi-seried3μm thick cuts were obtained in a rotating microtome and fixed on previously treated histological slides (sylanized). The pancreas cuts were submitted to the streptavidin-peroxidase technique to detect glucagon antigens present in the pancreas parenchyma of jurará. Quantitative analysis of the slides was made by counting 20 distinct fields containing glucagon immunoreactive cells under an optical microscope with a 40X objective lens. Glucagon immunoreactive cells were identified in the pancreas parenchymaof the mud turtle, and they were more frequent in the pancreatic islets. They were also observed in the exocrine parenchyma and in the epithelial of the pancreatic duct cover. Histological analysis showed that the pancreatic is lets did not present a capsule of conjunctive tissue. Quantitative analysis showed glucagon immunoreactive cells per field an average of cells 21.4 ± 11.4. Glucagon immmunoreactive cells were observed throughout the organ.




Testudinata. Parenchyma. Pancreaticislet. Chelonia.

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Acuña-Mesen, R.A. (1994). Morphometric variation and ecologic characteristic of the habitat of the Kinosternon scorpioides turtle in Costa Rica. Revista Brasileira de Biologia, 3, 537-547.

Brasil. Instrução Normativa IBAMA 169, de 20 de fevereiro de 2008. Aprova a Estrutura Regimental do IBAMA, publicada no Diário Oficial da União de 27 de abril de2007

Beisser, C.J., Weisgram, J., Hilgres, H., & Splechtna, H. (1998). Fine structure of the dorsal lingual epithelium of Trachemys scripta Elegans (Chelonia: Emydidae). The Anatomical Record, 2, 127-135. doi: 10.1002/(SICI)1097-0185(199802)250:2<127::AID-AR1>3.0.CO;2-0.

Garcia, A.A., Lozano, M.T., & Agulleiro, B. (1987). Endocrine pancreas of Testudo graeca L. (Chelonia) in summer and winter: an immunocytochemical and ultrastructural study. General and Comparative Endocrinology, 2, 235-248. doi: 10.1016/0016-6480(87)90035-9.

Gapp, D.A., Kenny, M.P., & Polak, J.M.(1985). The gastro-entero-pancreatic system of the turtle, Chrysemys picta. Peptídes, 3, 347-352. doi: 10.1016/0196-9781(85)90396-1.

Miller, M.R., & Lagios, M.D. (1970). The pancreas. (New York: Academic Press)

Muniz, W.W., Machado, V.L., & Marques, M. (1991). Immunohistochemical localization of insulin-like material in antral gastric mucosa and intestinal epithelial cells of the turtles Chrysemys dorbigni and Phrynops hilarii. Brazilian Journal of Medical and Biological Research, 3, 327-331.

Ivanova, V.F., Rossol’Ko, G.N., & Puzyrev, A.A. (1997). The endocrine apparatus of the epithelium of the gastric mucosa in the steppe turtle. Morfologiia, 1, 85-90

Madrid, J.F., Ballesta, J., Pastor, L.M., Perez-Tomaz,R., & Hernandez, F.(1989) Distribution of mucins in the mucosa of the digestive tract of reptiles: a histochemical study. Acta Histochemica, 85, 117-129. doi: 10.1016/S0065-1281(89)80053-4.

Pereira, J.G.(2000).Estudos histológico e histoquímico do tubo digestivo e do pâncreas do Kinosternon scorpioides Linnaeus, 1766 (Reptilia, Chelonia, kinosternidae), mudturtle. Dissertation (Master's in Veterinary Medicine).

Rhoten, W. (1987). Co-localization of glucagon and pancreatic polypeptide in testudine pâncreas. Experientia, 43, 28-430. doi:10.1007/BF01940439.

Tafuri, W.L., Santos, R.D.E.L.,Arantes, R.M., Goncalves, R., Melo, M.N., Michalick, M.S., & Tafuri, W.L. (2004). An alternative immunohistochemical method for detecting Leishmania amastigotes in paraffin-embedded canine tissues. Journal of Immunological Methods, 292, 17-23. doi: 10.1016/j.jim.2004.05.009.

Sousa, A.L., Campos-Junior, P.H.A., Costa, G.M.J., & França, L.R. (2014) Spermatogenic cycle length and sperm production in the Freshwater turtle Kinosternon scorpioides. ‎Biology of Reproduction,90, 1-10. doi: 10.1095/biolreprod.113.112391.

Viana, D.C., Anunciação, A.R.A., Santos, A.C., Oliveira, C.A., Miglino, M.A., Assis-Neto, A.C., Oliveira, A.S., & Sousa, A.L. (2014). Plasma Testosterone and Seasonal Reproductive Changes in the Scorpion Mud Turtle. Pakistan journal of zoology, 3, 620-28

Chandavar, V.R., & Naik, P.R. (2008). Immunocytochemical detection of glucagon and insulin cells in endocrine pancreas and cyclic disparity of plasma glucose in the turtle Melanochelystrijuga. Journal of Biosciences, 2, 239-247. doi: 10.1007/s12038-008-0041-8.



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